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Summary of G2/M transition


Cyclin B is synthesized in the end of S phase and associated with cdc2. CyclinB/cdc2 complex is held inactive during G2 phase and become active during M phase mainly because of Thr 161 phosphorylation by CyclinH/cdc7 complex, and Thr14 and Tyr15 dephosphorylation by CDC25 phoaphatase. (Contributed by Dr. Koji Nakade)


Reporters

Catalog no.Name of cloneShort description
RDB13080pCAG-Fucci2aFucci2a cell cycle reporter.
RDB13081pROSA-floxNeo-Fucci2aFucci2a cell cycle reporter. Targeting vector for R26 locus.

LocusRDB no.TypeName of cloneRunning title
chk12949DNA clonechk1+Plasmid clone of fission yeast Chk1
Chek16946H3042B08Chek1, NIA/NIH Mouse 15K cDNA Clone (full CDS)
CDC25C5908DNA clonepKM2L-phCDC25C(2)Promoter Bank clone, Human cdc25C promoter
CDC25C5841ShuttleVectorpAxit-phCDC25C-rLuc (R)Shuttle vector to generate recombinant adenovirus harboring renilla luciferase reporter.
CDC25C5840ShuttleVectorpAxit-phCDC25C-rLuc (F)Shuttle vector to generate recombinant adenovirus harboring renilla luciferase reporter.
CDC25C5468DNA clonepKM2L-phCDC25CPromoter Bank clone, Human cdc25C promoter
CDC25B2668ShuttleVectorpAxCALNLhCDC25B (reverse)Shuttle vector to produce rAd expressing human CDC25B
CDC25B2667ShuttleVectorpAxCALNLhCDC25B (forward)Shuttle vector to produce rAd expressing human CDC25B
CDC25B2334ShuttleVectorpAxCAhCDC25B (reverse)Shuttle vector to produce rAd expressing human CDC25B
CDC25B2333ShuttleVectorpAxCAhCDC25B (forward)Shuttle vector to produce rAd expressing human CDC25B
CDC25B1203DNA cloneCDC25Hu2Human cdc 25B cDNA
CDC25A5476DNA clonepKM2L-phCDC25APromoter Bank clone, Human cdc25A promoter
CDC25A4653ShuttleVectorpAxCALNLhCDC25A(forward)Shuttle vector to generate rAd expressing human CDC25A
CDC25A4646ShuttleVectorpAxCALNLhCDC25A(reverse)Shuttle vector to generate rAd expressing human CDC25A
cdc21570DNA clonepIBI delta Xe-cdc2-33RXenopus cdc2 (kinase-deficient) cDNA
cdc21569DNA clonepIBI delta Xe-cdc2Xenopus cdc2 cDNA
CCNH6416DNA clonepCMFlag_hsCCNHExpression vector of human CCNH
CCNH4093ShuttleVectorpAxCALNLhCyclin HShuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNH3510ShuttleVectorpAxCALNLhCyclin H(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCND34589DNA cloneSEREX clone NGO-Br-20 (ID 859) #1SEREX clone NGO-Br-20 (ID 859) #1
CCNB26415DNA clonepCMFlag_hsCCNB2Expression vector of human CCNB2
CCNB25448ShuttleVectorpAxCALNLhCCNB2(reverse)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB25447ShuttleVectorpAxCALNLhCCNB2(forward)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB25430ShuttleVectorpAxCALNLhCCNB2(forward)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB25425ShuttleVectorpAxCALNLhCCNB2(reverse)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB25424ShuttleVectorpAxCALNLhCCNB2(forward)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB25367ShuttleVectorpAxCALNLhCCNB2(reverse)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB25366ShuttleVectorpAxCALNLhCCNB2(forward)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB24098ShuttleVectorpAxCALNLhCCNB2Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNB23515ShuttleVectorpAxCALNLhCCNB2(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone

(GSB0010e, T.M. 2004.04.07)

2014.09.12