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p53 dependent and independent G1 arrest


Summary of p53 dependent and independent G1 arrest in responce to stress

DNA damaging agent activates the kinases named Atm and Atr that phosphorylate chk1 and chk2. The phosphorylation of p53 on ser20 by chks induces p53 stabilization by inhibiting MDM2 binding. p21induced by p53 binds to Cyclin E/cdk2 complex to make it inactive form.Chks also help ubiquitination and degradation of CDC25A that is required for activation of Cyclin E/cdk2 complex. (Contributed by Dr. Koji Nakade)


Reporters

Catalog no.Name of cloneShort description
RDB13080pCAG-Fucci2aFucci2a cell cycle reporter.
RDB13081pROSA-floxNeo-Fucci2aFucci2a cell cycle reporter. Targeting vector for R26 locus.

LocusRDB no.TypeName of cloneRunning title
Trp531285DNA clonep53-LTR pUC18 (Anti Sense#9)Expression vector, Antisense p53, cDNA
Trp531284DNA clonep53-LTR pUC18 (Sense#3)Expression vector, Sense p53, cDNA
TP535507DNA clonepKM2L-phP53Promoter Bank clone, Human p53 promoter
TP533187Recombinant adenovirusAxCAhp53KP1065Recombinant adenovirus harboring human p53 cDNA (Ser96Pro)
TP533186Recombinant adenovirusAxCAhp53KP1010Recombinant adenovirus harboring human p53 cDNA (Gly105Asp)
TP533185Recombinant adenovirusAxCAhp53KP1007Recombinant adenovirus harboring human p53 cDNA (Gly374Cys)
TP533184Recombinant adenovirusAxCAhp53KP1094Recombinant adenovirus harboring human p53 cDNA (Pro89His)
TP533183Recombinant adenovirusAxCAhp53KP1070Recombinant adenovirus harboring human p53 cDNA (Ser240Asn)
TP532807Recombinant adenovirusAxCALNLhp53R273H (forward)Recombinant adenovirus harboring human p53 cDNA (R273H)
TP532802Recombinant adenovirusAxCALNLhp53.clone 28 (forward)Recombinant adenovirus harboring human p53 cDNA (deletion)
TP532799Recombinant adenovirusAxCALNLhp53P70 (forward)Recombinant adenovirus harboring human p53 cDNA (Pro128Thr)
TP532798Recombinant adenovirusAxCALNLhp53P68 (forward)Recombinant adenovirus harboring human p53 cDNA
TP532795Recombinant adenovirusAxCALNLhp53WT (forward)Recombinant adenovirus harboring human p53 cDNA
TP532785Recombinant adenovirusAxCAhp53KP1070 (forward)Recombinant adenovirus harboring human p53 cDNA (Ser240Asn)
TP532784Recombinant adenovirusAxCAhp53KP1065 (forward)Recombinant adenovirus harboring human p53 cDNA (Ser96Pro)
TP532783Recombinant adenovirusAxCAhp53KP1010 (forward)Recombinant adenovirus harboring human p53 cDNA (Gly105Asp)
TP532782Recombinant adenovirusAxCAhp53KP1007 (forward)Recombinant adenovirus harboring human p53 cDNA (Gly374Cys)
TP532771Recombinant adenovirusAxCAhp53.clone 28 (forward)Recombinant adenovirus harboring human p53 cDNA (deletion)
TP532757Recombinant adenovirusAxCAhp53P70 (forward)Recombinant adenovirus harboring human p53 cDNA (Pro128Thr)
TP532756Recombinant adenovirusAxCAhp53P68 (forward)Recombinant adenovirus harboring human p53 cDNA
TP532755Recombinant adenovirusAxCAhp53WT (forward)Recombinant adenovirus harboring human p53 cDNA
TP532713ShuttleVectorpAxCALNLhp53R273H (reverse)Shuttle vector to produce rAd expressing human p53 (R273H)
TP532712ShuttleVectorpAxCALNLhp53R273H (forward)Shuttle vector to produce rAd expressing human p53 (R273H)
TP532630ShuttleVectorpAxCAhp53R273H (reverse)Shuttle vector to produce rAd expressing human p53 (R273H)
TP532629ShuttleVectorpAxCAhp53R273H (forward)Shuttle vector to produce rAd expressing human p53 (R273H)
TP532509ShuttleVectorpAxCAhp53P26 (reverse)Shuttle vector to generate rAd harboring human p53
TP532508ShuttleVectorpAxCAhp53P26 (forward)Shuttle vector to produce rAd expressing human p53
TP532507ShuttleVectorpAxCAhp53P25 (reverse)Shuttle vector to generate rAd harboring human p53
TP532506ShuttleVectorpAxCAhp53P25 (forward)Shuttle vector to produce rAd expressing human p53
TP532505ShuttleVectorpAxCAhp53P24 (reverse)Shuttle vector to generate rAd harboring human p53
TP532504ShuttleVectorpAxCAhp53P24 (forward)Shuttle vector to produce rAd expressing human p53
TP532503ShuttleVectorpAxCAhp53P21 (reverse)Shuttle vector to generate rAd harboring human p53
TP532502ShuttleVectorpAxCAhp53P21 (forward)Shuttle vector to produce rAd expressing human p53
TP532501ShuttleVectorpAxCAhp53P19 (reverse)Shuttle vector to generate rAd harboring human p53
TP532500ShuttleVectorpAxCAhp53P19 (forward)Shuttle vector to produce rAd expressing human p53
TP532499ShuttleVectorpAxCAhp53P17 (reverse)Shuttle vector to generate rAd harboring human p53
TP532498ShuttleVectorpAxCAhp53P17 (forward)Shuttle vector to produce rAd expressing human p53
TP532497ShuttleVectorpAxCAhp53P16 (reverse)Shuttle vector to generate rAd harboring human p53
TP532496ShuttleVectorpAxCAhp53P16 (forward)Shuttle vector to produce rAd expressing human p53
TP532495ShuttleVectorpAxCAhp53P50 (reverse)Shuttle vector to generate rAd harboring human p53
TP532494ShuttleVectorpAxCAhp53P50 (forward)Shuttle vector to produce rAd expressing human p53
TP532493ShuttleVectorpAxCAhp53P13 (reverse)Shuttle vector to generate rAd harboring human p53
TP532492ShuttleVectorpAxCAhp53P13 (forward)Shuttle vector to produce rAd expressing human p53
TP532437DNA clonepCAGhp53R273H (Reverse)Mammalian vector expressing mutant of p53 gene (R273H)
TP532436DNA clonepCAGhp53P70 (Reverse)Mammalian vector expressing mutant of p53 gene (Pro128Thr)
TP532435DNA clonepCAGhp53P68 (Forward)Mammalian vector expressing mutant of p53 gene (Ile 162 Val, Gly 266 Asp, frame shift)
TP532434DNA clonepCAGhp53WT (Reverse)Mammalian vector expressing wild type of p53 gene
TP532433DNA clonepCAGhp53R273H (Forward)Mammalian vector expressing mutant of p53 gene (R273H)
TP532432DNA clonepCAGhp53clone28 (Forward)Mammalian vector expressing the deletion mutant of p53 gene
TP532431DNA clonepCAGhp53P70 (Forward)Mammalian vector expressing mutant of p53 gene (Pro128Thr)
TP532430DNA clonepCAGhp53P68 (Reverse)Mammalian vector expressing mutant of p53 gene (Ile 162 Val, Gly 266 Asp, frame shift)
TP532429DNA clonepCAGhp53WT (Forward)Mammalian vector expressing wild type p53 gene
TP531982Recombinant AdenovirusAxCALNLhp53Recombinant adenovirus expressing human p53
MDM22198Recombinant AdenovirusAxCAhMDM2Recombinant adenovirus expressing human MDM2 regulated by CAG promoter
chk12949DNA clonechk1+Plasmid clone of fission yeast Chk1
Chek16946H3042B08Chek1, NIA/NIH Mouse 15K cDNA Clone (full CDS)
CDKN1A5893DNA clonepKM2L-phP21Promoter Bank clone, Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 promoter
CDKN1A3576ShuttleVectorpAxCALNLhp21(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CDKN1A1999ShuttleVectorpAxCAhp21Shuttle vector to generate rAd expressing human CDKI p21
CDK25250ShuttleVectorpAxCALNLhCDK2(reverse)Shuttle vector to generate rAd expressing human CDK2
CDK25249ShuttleVectorpAxCALNLhCDK2(forward)Shuttle vector to generate rAd expressing human CDK2
CDK24061ShuttleVectorpAxCALNLhCDK2Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CDK23492ShuttleVectorpAxCALNLhCDK2(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CDK23472ShuttleVectorpAxCALNLhCDK2(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
Cdk26947H3117F10Cdk2, NIA/NIH Mouse 15K cDNA Clone (full CDS)
CDC25A5476DNA clonepKM2L-phCDC25APromoter Bank clone, Human cdc25A promoter
CDC25A4653ShuttleVectorpAxCALNLhCDC25A(forward)Shuttle vector to generate rAd expressing human CDC25A
CDC25A4646ShuttleVectorpAxCALNLhCDC25A(reverse)Shuttle vector to generate rAd expressing human CDC25A

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(GSB0009e, T.M. 2004.04.14)

2014.09.12