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G1-S trasition


Summary of Cyclin function that regulates G1-S trasition

Cyclin Ds are upregulated in response to Ras-Map kinase signalling and assembled with cyclin D-dependent kinases (CDKs). The CDKs initiate Rb phosphorylation, releasing E2F from negative constraints and facilitating activation of a series of E2F-responsive genes, the products of which are necessary for S-phase entry. Activation of cyclin E by E2F enables formation of the cyclin E-cdk2 complex. This is accelerated by the continued sequestration of Cip/Kip proteins into complexes with assembling cyclin D-cdk complexes. Cyclin E-cdk2 completes the phosphorylation of Rb, further enabling activation of E2F-responsive genes, including cyclin A. Cyclin E-Cdk2 also stimulates DNA synthesis presumably by facilitating origin firing through Cdc45-dependent loading of DNA polymerase and promoting histone biosynthesis through direct phosphorylation of NPAT, a factor required for histone transcription. (Contributed by Dr. Koji Nakade)


Reporters

Catalog no.Name of cloneShort description
RDB13080pCAG-Fucci2aFucci2a cell cycle reporter.
RDB13081pROSA-floxNeo-Fucci2aFucci2a cell cycle reporter. Targeting vector for R26 locus.

LocusRDB no.TypeName of cloneRunning title
RB11278DNA clonepTZRb1.05Human Rb cDNA (amino acid 282-633)
PPP3CA4132ShuttleVectorpAxCALNLhCalAalphaShuttle vector to generate rAd harboring Sugano full-length cDNA clone
PPP3CA3559ShuttleVectorpAxCALNLhCalAalpha(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
MYC6671DNA clonepCMFlag_hsc-MycExpression vector of human c-Myc
MYC6435DNA clonepENTR/hc-Myc
MYC6432DNA clonepGEM3Zf_hsc-MycPlasmid clone of human c-Myc cDNA
MYC6428DNA clonepGEM3Zf_hsc-Myc(alternative translation initiations from an upstream)Plasmid clone of human MYC (alternative translation initiations from an upstream) cDNA
MYC4659ShuttleVectorpAxCALNLhc-Myc(forward)Shuttle vector to generate rAd expressing human MYC
MYC4650ShuttleVectorpAxCALNLhc-Myc(reverse)Shuttle vector to generate rAd expressing human MYC
Myc2958DNA clonemouse c-mycPlasmid clone of mouse c-myc cDNA, PCR fragment
E2F15537DNA clonepKM2L-phE2F1(2)Promoter Bank clone, Human transcription factor E2F1 (E2F1) promoter
E2F15483DNA clonepKM2L-phE2F1(1)Promoter Bank clone, Human transcription factor E2F1 (E2F1) promoter
Cdk46971H3147D06Cdk4, NIA/NIH Mouse 15K cDNA Clone (full CDS)
CDC25A5476DNA clonepKM2L-phCDC25APromoter Bank clone, Human cdc25A promoter
CDC25A4653ShuttleVectorpAxCALNLhCDC25A(forward)Shuttle vector to generate rAd expressing human CDC25A
CDC25A4646ShuttleVectorpAxCALNLhCDC25A(reverse)Shuttle vector to generate rAd expressing human CDC25A
CCNH6416DNA clonepCMFlag_hsCCNHExpression vector of human CCNH
CCNH4093ShuttleVectorpAxCALNLhCyclin HShuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNH3510ShuttleVectorpAxCALNLhCyclin H(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCND34589DNA cloneSEREX clone NGO-Br-20 (ID 859) #1SEREX clone NGO-Br-20 (ID 859) #1
Camk2g6890DNA cloneH4064B06Camk2g, NIA/NIH Mouse 7.4K cDNA Clone (full CDS)
CAMK2B4139ShuttleVectorpAxCALNLhCAMK2BShuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B4136ShuttleVectorpAxCALNLhCAMK2BShuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B4131ShuttleVectorpAxCALNLhCAMK2BShuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B3568ShuttleVectorpAxCALNLhCAMK2B(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B3563ShuttleVectorpAxCALNLhCAMK2B(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B3558ShuttleVectorpAxCALNLhCAMK2B(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK1G4142ShuttleVectorpAxCALNLhCAMK1GShuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK1G3571ShuttleVectorpAxCALNLhCAMK1G(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
Camk1g1782DNA cloneCaM kinase I gammaRat calmodulin kinase I gamma cDNA

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(GSB0008e, T.M. 2004.04.07)

2014.09.12