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G1 cdks and cdk inhibitors


Somatic cell cycles consist of alternating DNA synthetic (S) and mitotic (M) phases, separated by gap phases (G1 and G2). Mammalian cells respond to extracellular mitogens and antiproliferative cytokines from the time that they exit mitosis (vertical arrow at top) until they reach the restriction point, after which they can complete the cell division cycle in the absence of extracellular growth factors. Cyclin D-dependent kinases accumulate in response to mitogenic signals and initiate the phosphorylation of Rb, a process that is completed by cyclin E-cdk2. Once cells enter S phase, cyclin E is degraded and cyclin A enters into complexes with cdk2. INK4 proteins oppose the activities of the various cyclin D-dependent kinases, whereas Cip/Kip proteins specifically inhibit cyclin E-cdk2 and cyclin A-cdk2. (Contributed by Dr. Koji Nakade)


Reporters

Catalog no.Name of cloneShort description
RDB13080pCAG-Fucci2aFucci2a cell cycle reporter.
RDB13081pROSA-floxNeo-Fucci2aFucci2a cell cycle reporter. Targeting vector for R26 locus.

LocusRDB no.TypeName of cloneRunning title
CDKN1B5508DNA clonepKM2L-phKIP1Promoter Bank clone, Human p27 cyclin-dependent kinase inhibitor (kip1) promoter
CDKN1B4645ShuttleVectorpAxCALNLhp27(forward)Shuttle vector to generate rAd expressing human CDKN1B
CDKN1B4642ShuttleVectorpAxCALNLhp27(reverse)Shuttle vector to generate rAd expressing human CDKN1B
CDKN1B2069ShuttleVectorpAxCAhp27 (As)Shuttle vector to generate rAd expressing human CDKI p27
CDKN1B2011ShuttleVectorpAxCAhp27Shuttle vector to generate rAd expressing human CDKI p27
CDKN1B2010Recombinant AdenovirusAxCAhp27Recombinant adenovirus expressing human CDKI p27 regulated by CAG promoter
Cdkn1b2856DNA cloneHA-P27Expression vector of HA-tagged mouse p27/Kip1
CDKN1A5893DNA clonepKM2L-phP21Promoter Bank clone, Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 promoter
CDKN1A3576ShuttleVectorpAxCALNLhp21(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CDKN1A1999ShuttleVectorpAxCAhp21Shuttle vector to generate rAd expressing human CDKI p21
Cdk46971H3147D06Cdk4, NIA/NIH Mouse 15K cDNA Clone (full CDS)
CDK25250ShuttleVectorpAxCALNLhCDK2(reverse)Shuttle vector to generate rAd expressing human CDK2
CDK25249ShuttleVectorpAxCALNLhCDK2(forward)Shuttle vector to generate rAd expressing human CDK2
CDK24061ShuttleVectorpAxCALNLhCDK2Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CDK23492ShuttleVectorpAxCALNLhCDK2(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CDK23472ShuttleVectorpAxCALNLhCDK2(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
Cdk26947H3117F10Cdk2, NIA/NIH Mouse 15K cDNA Clone (full CDS)
CDC25C5908DNA clonepKM2L-phCDC25C(2)Promoter Bank clone, Human cdc25C promoter
CDC25C5841ShuttleVectorpAxit-phCDC25C-rLuc (R)Shuttle vector to generate recombinant adenovirus harboring renilla luciferase reporter.
CDC25C5840ShuttleVectorpAxit-phCDC25C-rLuc (F)Shuttle vector to generate recombinant adenovirus harboring renilla luciferase reporter.
CDC25C5468DNA clonepKM2L-phCDC25CPromoter Bank clone, Human cdc25C promoter
CDC25B2668ShuttleVectorpAxCALNLhCDC25B (reverse)Shuttle vector to produce rAd expressing human CDC25B
CDC25B2667ShuttleVectorpAxCALNLhCDC25B (forward)Shuttle vector to produce rAd expressing human CDC25B
CDC25B2334ShuttleVectorpAxCAhCDC25B (reverse)Shuttle vector to produce rAd expressing human CDC25B
CDC25B2333ShuttleVectorpAxCAhCDC25B (forward)Shuttle vector to produce rAd expressing human CDC25B
CDC25B1203DNA cloneCDC25Hu2Human cdc 25B cDNA
CDC25A5476DNA clonepKM2L-phCDC25APromoter Bank clone, Human cdc25A promoter
CDC25A4653ShuttleVectorpAxCALNLhCDC25A(forward)Shuttle vector to generate rAd expressing human CDC25A
CDC25A4646ShuttleVectorpAxCALNLhCDC25A(reverse)Shuttle vector to generate rAd expressing human CDC25A
cdc21570DNA clonepIBI delta Xe-cdc2-33RXenopus cdc2 (kinase-deficient) cDNA
cdc21569DNA clonepIBI delta Xe-cdc2Xenopus cdc2 cDNA
CCNH6416DNA clonepCMFlag_hsCCNHExpression vector of human CCNH
CCNH4093ShuttleVectorpAxCALNLhCyclin HShuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNH3510ShuttleVectorpAxCALNLhCyclin H(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCND34589DNA cloneSEREX clone NGO-Br-20 (ID 859) #1SEREX clone NGO-Br-20 (ID 859) #1
CCNB26415DNA clonepCMFlag_hsCCNB2Expression vector of human CCNB2
CCNB25448ShuttleVectorpAxCALNLhCCNB2(reverse)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB25447ShuttleVectorpAxCALNLhCCNB2(forward)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB25430ShuttleVectorpAxCALNLhCCNB2(forward)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB25425ShuttleVectorpAxCALNLhCCNB2(reverse)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB25424ShuttleVectorpAxCALNLhCCNB2(forward)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB25367ShuttleVectorpAxCALNLhCCNB2(reverse)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB25366ShuttleVectorpAxCALNLhCCNB2(forward)Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB24098ShuttleVectorpAxCALNLhCCNB2Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNB23515ShuttleVectorpAxCALNLhCCNB2(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNA26665DNA clonepCMFlag_hsCCNA2Expression vector of human CCNA2/Cyclin A2
CCNA24162ShuttleVectorpAxCALNLhCyclin A2 Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNA23595ShuttleVectorpAxCALNLhCyclin A2 (forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNA15482DNA clonepKM2L-phCYCA1Promoter Bank clone, Human cyclin A1 promoter
CCNA13557ShuttleVectorpAxCALNLhCCNA1(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNA13534ShuttleVectorpAxCALNLhCCNA1(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
BCCIP2617Recombinant adenovirusAxCALNLhTOK-1 alpha recombinant adenovirus expressing human TOK-1 alpha
BCCIP2346Recombinant AdenovirusAxCAhTOK-1 alphaRecombinant adenovirus expressing human TOK-1 alpha

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(GSB0007e, T.M. 2004.04.05)

2014.09.12