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36/C11 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 36/C11
NCBI Gene ID 2539317
Gene Symbol atg20
Protein Name sorting nexin Atg20
Synonym SPCC16A11.08
RefSeq Protein
PomBase(1) SPCC16A11.08
KEGG(2) spo:SPCC16A11.08
Depositor-supplied data(3) 36C11
Note 893C:T / 921A:G / 960T:C / 1135T:C / 1404A:G / 1496A:G / 1720A:G(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW014459 SpENT36C11 confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW054459 SpFFH36C11 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW094459 SpYFH36C11 confirmation required
user_report - reference Zhao, D., Atg20- and Atg24-family proteins promote organelle autophagy in fission yeast. J. Cell Sci. 129 (22): 4289-4304 (2016). PMID 27737912.

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.11.07

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl