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35/F03 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 35/F03
NCBI Gene ID 2538741
Gene Symbol ynd1
Protein Name nucleoside diphosphatase Ynd1
Synonym SPCC11E10.05c
RefSeq Protein NP_588201.1
PomBase(1) SPCC11E10.05c
KEGG(2) spo:SPCC11E10.05c
Depositor-supplied data(3) 35F03
Note  

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW014123 SpENT35F03 confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW054123 SpFFH35F03 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW094123 SpYFH35F03 Known as Golgi marker (Matsuyama, A., Nat Biotechnol 24(7):841–847, 2006; Li, G. Proc. Natl. Acad. Sci. U.S.A. 114 (3): E376-E385, 2017).
user_report - reference Li, G., Characterization of cytopathic factors through genome-wide analysis of the Zika viral proteins in fission yeast. Proc. Natl. Acad. Sci. U.S.A. 114 (3): E376-E385 (2017). PMID 28049830.

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.

2023.11.07

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl