Please review the QC test results indicated by icon below as well as clone information before placing your order.

pETDuet-2B4-2B2_dE310K

Bacterial expression vector of human eIF2Bdelta mutant (E310K) and human eIF2Bbeta.

Distribution information

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18641_B0Lbp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamHI_R (Pr0065) Region: lacO, RBS, insert 5' (2B4 5') Sequence file: RDB18641_B0Lba.seq
>D07570A3_B0Lb_3_pBR322_BamHI_R_D03_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGANTNNC NATAGNNNGA NTGTGAGCGG NNAACAATTC    61 CCCTCTAGAA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACCATGGGC AGCAGCAGCC   121 AGGATCCGAA TTCGAGCTCG GCGCGCCTGC AGGTCGACGC TGCTGTGGCC GTGGCTGTTC   181 GCGAGGACTC GGGATCCGGG ATGAAGGCGG AGCTTCCCCC CGGGCCTGGG GCAGTGGGGA   241 GGGAAATGAC CAAAGAAGAA AAGCTGCAGC TTCGGAAGGA AAAGAAACAG CAGAAGAAGA   301 AACGGAAGGA AGAAAAGGGG GCAGAACCAG AGACTGGCTC TGCTGTATCT GCAGCCCAAT   361 GTCAAGTAGG CCCAACCAGA GAACTGCCAG AATCGGGCAT TCAGTTGGGC ACTCCTCGGG   421 AGAAAGTTCC AGCTGGTCGG AGTAAGGCCG AACTTCGGGC TGAGCGTCGA GCCAAGCAGG   481 AGGCCGAGCG GGCCCTGAAA CAGGCAAGAA AAGGGGAACA AGGAGGACCA CCTCCTAAGG   541 CCAGCCCCAG CACAGCTGGA GAAACCCCCT CAGGAGTGAA GCGTCTCCCT GAGTACCCTC   601 AGGTTGATGA CCTACTTCTG AGAAGGCTTG TTAAAAAACC AGAGCGTCAA CAGGTTCCTA   661 CACGAAAGGA TTATGGATCC AAAGTCAGTC TCTTCTCTCA CCTACCCCAG TACAGCAGAC   721 AAAACTCTCT GACCCAGTTT ATGAGCATCC CATCCTCTGT GATCCACCCA GCCATGGTGC   781 GACTCGGCCT GCAGTACTCC CAGGGCCTGG TCAGTGGCTC CNNTGCCCGG NGTATTGCCC   841 TGCTTCGTGC CTTGCANCAN GTGATTCANG ATTNNNNNNC ACCGCCTAAT GAANAANTCT   901 CCNGGGNTCT AGNGAATAAA CTAAANNCCT ACNNGANCTT NCTGANTCAG NGNCGTCCCN 961 NGTCANCGAG NATGNNNNNN NNNN //
Primer: T7 terminator (Pr0155) Region: insert 3' (2B2 3') Sequence file: RDB18641_B0Lbb.seq
>D07570A3_B0Lb_3_T7 terminator_E03_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNCNNN NANNNNANTC NNTGTGCTGG CGTTCAAATT TCGCAGCAGC    61 GGTTTCTTTA CCAGACTCNN GGGTACCGAC GTCTCATAAA ACATGATCAT CAGGATGGTA   121 GAGTTCACTC ATCAGGCGGT AGATGTAGGA AGGTGCATTC CCACCAATGT TGGAGATAAA   181 GAGGGTAATG AGCTCTGGGG GAACGTAGTC AAACACAGGG CAATGCACGC TGACCTTCTC   241 CAGAATGTCC CCTTCTGTGA ATGGCAGGAC TTCTTCAGGA GCCACAAACT TATGAAATGA   301 GTCTTCTTCA TTGGGGAACT GTGGAGAAAG TTTGAACATA GGTGCACAGA CGATGAGTGG   361 GGTGGAATGG TGTTTTGCTG CCAGTGCCAG AGTGTGAGTT CCTGTCACAG CTCTCAGGGC   421 CCCATTGGCC AGGATGGTCT TCGTGCCAAT GATCACCTTG TTGACTCTTG ACATAACGGC   481 AAAAATGGCA GCATCAGTCA TGACAGTTGT CTCAATACCT GCTTTGGACA AATTCACAGC   541 CATTTCATGA CCCTGGCAGA AAGGAGCACA CTCTGCTACA ATGACATGGA ATTTCCTCTT   601 TCGGGCAGCC TCTTTGAGGA AGGCCTCTAC TGTTCGGGAG AAGCCAATGG TCATGATCAC   661 CTCATTGGAG TGAATGTGCT CCAGAGCCTG GGCTGCAATG TTCTCCATTG TCCCTTCCAG   721 CTCCACTAGC AGCTCATTAA TCGCCTCAAT GATGTTGGAC TGGAGTTGGG CATAATGNAA   781 GCTGAAATCC TCGTTNANGC CTCCGGATGT CAACAGTTTG TGCAGGGACT CCTGCTGATC   841 ACTCTCGTCG CTGCGNCCAT GGAGTCTGCC ATACTCCTCC CGGATAATCT TGANCANTCT   901 CCGCANNATG TNGNCCACGG NGGTCTCNGA NGGNTGANCG GCNNNCNTCC NNCTGNCNNC 961 TCTGNNNATC ANCTCCNTCA NCTNCCCCNC GNNGNTNCNN CNGNNNNNNN NNN //
Primer: hEIF2B-M_F1 (Pr0935) Region: insert mid (2B4 E310K) Sequence file: RDB18641_B0Lbc.seq
>D07570A3_B0Lb_3_hEIF2B-M_F1_F03_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC CCNNNNNNNT GAAGCGTCTC CCTGANTACC    61 CTCAGGTTGA TGACCTACTT CTGAGAAGGC TTGTTAAAAA ACCAGAGCGT CAACAGGTTC   121 CTACACGAAA GGATTATGGA TCCAAAGTCA GTCTCTTCTC TCACCTACCC CAGTACAGCA   181 GACAAAACTC TCTGACCCAG TTTATGAGCA TCCCATCCTC TGTGATCCAC CCAGCCATGG   241 TGCGACTCGG CCTGCAGTAC TCCCAGGGCC TGGTCAGTGG CTCCAATGCC CGGTGTATTG   301 CCCTGCTTCG TGCCTTGCAG CAGGTGATTC AGGATTACAC AACACCGCCT AATGAAGAAC   361 TCTCCAGGGA TCTAGTGAAT AAACTAAAAC CCTACATGAG CTTCCTGACT CAGTGCCGTC   421 CCCTGTCAGC GAGCATGCAC AACGCCATCA AGTTCCTTAA CAAGGAAATC ACCAGTGTGG   481 GCAGTTCCAA GCGGGAAGAG GAGGCCAAGT CAGAACTTCG AGCAGCCATT GATCGGTATG   541 TGCAAAAGAA GATTGTGCTA GCAGCTCAGG CAATTTCACG CTTTGCTTAC CAGAAGATCA   601 GTAATGGAGA TGTGATCCTG GTATATGGAT GCTCATCTCT GGTATCACGA ATTCTTCAGG   661 AGGCTTGGAC AGAGGGCCGG CGGTTTCGGG TGGTAGTGGT GGACAGCCGG CCATGGCTGG   721 AAGGAAGGCA CACACTACGT TCTCTAGTCC ATGCTGGTGT CCCAGCCTCC TACCTGCTGA   781 TTCCTGCAGC CTCCTATGTG CTCNNNNANG TTTCCAAGGT GCTATTGGGA NCTCATGCAC   841 TCTTGGCCNA CGGGTCTGTG ATGTCACGGG TANGGANAGC ACAGTTAGCC CTGNTGGCTC   901 GAGCCCATAA TGNACCAGNG CTGGTTTGCT GTGAAACNTA CAAGTTCTGT GANCGTGTGC   961 ANNNTNNTNN CTTTNGTNTN NAATGANCTA NATGANCNTN NNNNATCTGC NNNGNNANNN 1021 NNNNNNN //
Primer: hEIF2Bb_R (Pr0902) Region: insert mid (2B2 5', RBS, lacO, T7pro, 2B4 3') Sequence file: RDB18641_B0Lbd.seq
>D07570A3_B0Lb_3_hEIF2Bb_R_G03_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT CCTCCTGCCC TCTCTGCGGN NCAGCTCCNT    61 CAGCTCCCCC GCGTTGCTCC NGCGGTGGTC CGTGATGATC TGGCGCAGCA ACCCTAGGGT   121 CTCCCGAGCC ATTTCCTCGG AGCTGCGCGG CCCACCACCC CGCTTCAGGG TCTCCACGAA   181 GCTCTCGATC CTCTCTGACA ACTCCGAGCC CTTCGCTGCG GATCCCGGCA TATGTATATC   241 TCCTTCTTAT ACTTAACTAA TATACTAAGA TGGGGAATTG TTATCCGCTC ACAATTCCCC   301 TATAGTGAGT CGTATTAATT TCGATTATGC GGCCGTGTAC AATACGATTA CTTTCTGTTC   361 GACTTAAGCA TTATGCGGCC GCTCACTGGT CACTGCTCTT GACTCGTAGA ACAACAGGTA   421 CAGAACTGCA AGGGATCATC CCCAGCTCCG TGATCACCAG ATCCACAAGC TCTGGGGGAG   481 TCACATCATA GACTAGATTC AACAACCGTA GGGATGCGTG GTTCTGCCAG TTAGCCAGCG   541 CAACATGTTC TCCCCGCTTA CATTGCAGAT CATCAGGGTC ATCTAGCTCA TTAGAGACAA   601 AGGCATCAGT CTGCACACGC TCACAGAACT TGTATGTTTC ACAGCAAACC AGCACTGGTA   661 CATTATGGGC TCGAGCCACC AGGGCTAACT GTGCTGTCCC TACCCGTGAC ATCACAGACC   721 CGTTGGCCAA GAGTGCATGA GCTCCCNATA GCACCTTGGA AACCTCTGGG AGCACATAGG   781 AGGCTGCAGG AATCANCANG TANGANGCTG GGACACCAGC ATGNACTANA GAACGTAGTG   841 TGTGCCTTNC TTNCAGCCAT GGCCGGNTGN NCNCCACTAC NACCCGAAAC CGCCGGCCCT   901 CTGTNCNNNC CTNCTGAAGA ATTCGTGATA CCAGANATGA NCATNCNNAT NNNNNNNTNA   961 CATCTCNNTN NCTGATCTTC TGGNNNNNAA AGCGNNNANN TGNNNNANCT NNTANCNNNN 1021 NNNNNN //
Primer: pGL4pA_R (Pr0664) Region: lacI Sequence file: RDB18641_B0Lbe.seq
>D07570A3_B0Lb_3_pGL4pA_R_H03_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNC CNNGNGNNNN NNNAGTGAGC TAACTTACNT TAATTGCGTT    61 GCGCTCACTG CCCGNNNNNN NNNNNGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG   121 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA   181 GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC   241 GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA   301 TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATGTCC GCACCAACGC   361 GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA   421 GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA   481 TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT   541 TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG   601 CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT   661 GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA   721 CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCNGCGGA TAGTTAATGA   781 TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACNG GCTTCGANGC   841 CGCTTCNTTC TACCATCGAC ACCACCACGC TGGCACCCAG TNGATCNGCG CGAGATTTAA   901 TCGCCGCGAC NATTTGNNAN GGCGCGNGNA GGGCNNNANT GNANGTGGNA NNNCNNATCA   961 NCNACNACNG NTNNNCCNNC ANNNGNNGNG CCNNGCNGNN NNGNNNTGNN NNNNANCTNC 1021 NGCCNNCNNN NNNTNNNNNN TTTTNNNNNN NNTTNNNNNN NANNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl