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pEGFP-C1-Rab34-T66N 

Expression vector of mouse Rab34 constitutively negative mutant.

Catalog number RDB18395
Resource name pEGFP-C1-Rab34-T66N 
Clone info. Expression vector of mouse Rab34 constitutively negative mutant, T66N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name mouse Rab34 cDNA
Reference of insert sequence AB232630 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab34

          Reference sequence
            
           
          Sequence (partial) RDB18395pka01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. J. Biol. Chem. 277, 12432-12436, 2002; Tamura, K. et al. Mol. Biol, Cell 20, 2900-2908, 2009)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. et al. J. Biol. Chem. 277, 12432-12436, 2002; Tamura, K. et al. Mol. Biol, Cell 20, 2900-2908, 2009)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB18395 pEGFP-C1-Rab34-T66N  Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Oguchi, M.E., A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis. J. Biol. Chem. 295 (36): 12674-12685 (2020). PMID 32669361.
          original Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Oguchi, M.E., A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis. J. Biol. Chem. 295 (36): 12674-12685 (2020). PMID 32669361.
          original Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694.
          reference Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727.

          2023.04.06

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