Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK381

A donor vector for introducing CMV-OsTIR1 (F74G mutant) at the human AAVS1 locus.

Sequence (full)	RDB18337zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18337_B0Gfp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMVpro, OstIR1(F74G) 5' Sequence file: RDB18337_B0Gfa.seq
>D07105A5_B0Gf_2_CMV_Forward_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTT GNNGCNNTGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA    61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTGCCACCAT GACATACTTT   121 CCTGAAGAGG TCGTCGAACA CATTTTTAGC TTCCTGCCTG CACAGAGAGA TAGAAACACA   181 GTGAGCCTGG TCTGCAAAGT GTGGTACGAG ATCGAACGCC TGAGCCGGAG AGGAGTGTTC   241 GTCGGCAACT GCTATGCTGT GAGAGCAGGC AGGGTCGCCG CTAGGTTTCC AAATGTGCGC   301 GCACTGACCG TCAAGGGGAA ACCCCACGGC GCCGACTTTA ACCTGGTGCC CCCTGATTGG   361 GGAGGATACG CCGGCCCTTG GATCGAGGCA GCCGCTCGCG GCTGTCATGG ACTGGAGGAA   421 CTGCGCATGA AGCGAATGGT GGTCTCTGAC GAAAGTCTGG AGCTGCTGGC TCGGAGCTTC   481 CCTAGGTTTC GCGCACTGGT GCTGATTTCT TGCGAAGGCT TCAGCACCGA TGGACTGGCA   541 GCCGTGGCCT CCCACTGTAA GCTGCTGCGG GAGCTGGACC TCCAGGAGAA TGAAGTGGAG   601 GATAGAGGCC CCAGATGGCT GTCTTGCTTC CCAGACTCAT GTACCAGCCT GGTGTCCCTG   661 AACTTTGCCT GCATCAAAGG CGAAGTGAAT GCTGGGTCCC TGGAGCGGCT GGTCTCAAGA   721 AGCCCCAACC TGAGGTCTCT GCGGCTGAAC CGGAGCGTGA GCGTGGACAC TCTGGCTAAG   781 ATTCTGCTGA NAACCCCTAA CCTGGANGAT CTGGGAACCG GCAATCTGAC AGANGATTTC   841 CAGANAGAAT CCTACTTTNA ACTGACTTCT GNCCTGNAGA ANTGNAAAAT GNTGANGANT   901 CTGTCNNNAT TCTGGNATGC TNCNCCCGNG TGCCTGANCT TTATCTACCC TCTGTGTGCA   961 CAGCTGANNN NNCTGNACCT GANCTATGCN NNNNNCCNNN NNGNNNNNGN TCNGNNNANN 1021 NNNNTNNNNN NNNNN //
Primer: PGK_N_rev (Pr0243) Region: loxP, SV40 pA, OsTIR1(F74G) 3' Sequence file: RDB18337_B0Gfb.seq
>D07105A5_B0Gf_2_PGK_N_rev_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNANANTTCN AGGTCGAGAT CCTAAGCTTG GCTGGACGTA    61 AACTCCTCTT CAGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT ATATTAAGGG   121 TTATTGAATA TGATCGCTTA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC   181 AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA   241 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG   301 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT   361 ATGATCTAGA GTCGCGGCCC CGGTGGATCC CGGGCCCGCG GTACCGTCGA CGGGCCCGGG   421 ATCCGATGGA TCCTCACAGA ATCTTCACAA AGTTGGGAGC ATCATCTCTT GCCCCTGCGG   481 TGGTCCGATA CACATACAGT TTCTCCACCT TTGGCAGGTC GCCATGATTT TCCTCCATTT   541 CGTTAGACCC GTCCCGCTCA TTAATGACTT CCACGTTCAG CATAGGCATT TTACTAGCCA   601 GGACCTGACA GCCCTTCAGA GTCACATTGC AACTAGACAT CCACAGGCTC CTCATTGTCT   661 CGTATCTGGC AAAGTTTCCC AGCAGAGCGG CATCCCCGAA AGGGCTGTCC CGGATTTCCA   721 GTTTTCGCAG ATTCTTACAC CCGTTCATCA CGTGCATCAT GCCCTTATCG GANTCTCCGG   781 NAAATGNNAT GNTCAGCATC TCCAGCTGCT TANCATACTT GCCGANGTAC NTGAANACTT   841 TGTCTGNCAN CANGNCNGAA ATGNTCANCC NCTNGANTCC CTTGNNCTCT CTCNCNATAG   901 CTNNNAACCC TTCATNCNGN GNNNGGGNNG NNANNNCNNN NGNNTNNNCN NNNNNNNNAN 961 NANNNANNNN AANNNGNNNN ANNNNNGNNN NNNNNNN //
Primer: reverse-2 (Pr0100) Region: AAVS1 arm1 Sequence file: RDB18337_B0Gfc.seq
>D07105A5_B0Gf_2_reverse-2_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCCTCN CTAAAGGGNN TAGTCCTGCA    61 GGTTTAAACG AATTCGCCCT TTGCTTTCTC TGACCAGCAT TCTCTCCCCT GGGCCTGTGC   121 CGCTTTCTGT CTGCAGCTTG TGGCCTGGGT CACCTCTACG GCTGGCCCAG ATCCTTCCCT   181 GCCGCCTCCT TCAGGTTCCG TCTTCCTCCA CTCCCTCTTC CCCTTGCTCT CTGCTGTGTT   241 GCTGCCCAAG GATGCTCTTT CCGGAGCACT TCCTTCTCGG CGCTGCACCA CGTGATGTCC   301 TCTGAGCGGA TCCTCCCCGT GTCTGGGTCC TCTCCGGGCA TCTCTCCTCC CTCACCCAAC   361 CCCATGCCGT CTTCACTCGC TGGGTTCCCT TTTCCTTCTC CTTCTGGGGC CTGTGCCATC   421 TCTCGTTTCT TAGGATGGCC TTCTCCGACG GATGTCTCCC TTGCGTCCCG CCTCCCCTTC   481 TTGTAGGCCT GCATCATCAC CGTTTTTCTG GACAACCCCA AAGTACCCCG TCTCCCTGGC   541 TTTAGCCACC TCTCCATCCT CTTGCTTTCT TTGCCTGGAC ACCCCGTTCT CCTGTGGATT   601 CGGGTCACCT CTCACTCCTT TCATTTGGGC AGCTCCCCTA CCCCCCTTAC CTCNNNNNNC   661 TGTGCTAGCT CTTCCAGCCC CCTGTCATGG CATCTTCCAG GGGTCCGAGA GCTCAGCTAG   721 TCTTCTTCCT CCAACCCGGG CCCCTATGTC CACTTCAGGA CAGCATGTTT GCTGCCTCCA   781 GGGATCCTGT GTCCCCGAGC TGGGANCACC TTATATTCCC ANGGCCGGTT AATGNGGCTC   841 TGGTTCTGGG TANTTTTATC TGNCCCCTCC ANCCCACANT GGGGNAAGCT ANTAATCAAT   901 TACGGGGTCA TTAGTTCATA NCCNNTATAT GGAGTTNNCN CGNTACNTNA CTTACNGTAA   961 ANGGCCCGCC TGGNTGANNG CCCNACNANC CCCGNCNNNT GACNTNANNN TGANGNATGT 1021 TNCNNNNNNN NNCGNCNANN NGGGANTTTC CATTNNNNNN NNNN //
Primer: pUC-M13_-100 (Pr0310) Region: AVVS1 arm2 Sequence file: RDB18337_B0Gfd.seq
>D07105A5_B0Gf_2_pUC-M13_-100_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN TGNGNANGCN NGGNTTTTCC CNGTCNCGAC GTTGTAAAAC    61 GACGGCCAGT GAATTGTAAT ACGACTCACT ATAGGGCGAA TTGAATTTAG CGGCCGCGAA   121 TTCGCCCTTA GAGCAGAGCC AGGAACCCCT GTAGGGAAGG GGCAGGAGAG CCAGGGGCAT   181 GAGATGGTGG ACGAGGAAGG GGGACAGGGA AGCCTGAGCG CCTCTCCTGG GCTTGCCAAG   241 GACTCAAACC CAGAAGCCCA GAGCAGGGCC TTAGGGAAGC GGGACCCTGC TCTGGGCGGA   301 GGAATATGTC CCAGATAGCA CTGGGGACTC TTTAAGGAAA GAAGGATGGA GAAAGAGAAA   361 GGGAGTAGAG GCGGCCACGA CCTGGTGAAC ACCTAGGACG CACCATTCTC ACAAAGGGAG   421 TTTTCCACAC GGACACCCCC CTCCTCACCA CAGCCCTGCC AGGACGGGGC TGGCTACTGG   481 CCTTATCTCA CAGGTAAAAC TGACGCACGG AGGAACAATA TAAATTGGGG ACTAGAAAGG   541 TGAAGAGCCA AAGTTAGAAC TCAGGACCAA CTTATTCTGA TTTTGTTTTT CCAAACTGCT   601 TCTCCTCTTG GGAAGTGTAA GGAAGCTGCA GCACCAGGAT CAGTGAAACG CACCAGACAG   661 CCGCGTCAGA GCAGCTCAGG TTCTGGGAGA GGGTAGCGCA GGGTGGCCAC TGAGAACCGG   721 GCAGGTCACG CATCCCCCCC TTCCCTCCCA CCCCCTGCCN AGCTCTCCCT CCCAGGATCC   781 TCTCTGGCTC CATCGTAAGC AAACCTTAGA GGTTCTGGNA AGGAGAGAGA TGGCTCCNGG   841 AAATGGGGGT GTGTCACCAG ATAANGAATC TGCCTAACAN GANGTGGGGG NTAGANCCAA   901 TATCNNGAGA NTANGAANGA NGANGNCTAN NGNNGGGGCT TTTCTGNCNC CNATCCTGTC   961 CCNANNAAAG CTTANNGAAN CTCTNCNANG GANCNAATAN CTTCNNATAG CATACNTTAT 1021 NNNAAGNNNT NNNNNNNNNT NNCNNNNNNN NNN //
Primer: PGKpro_F (Pr0249) Region: PuroR, mouse PGK ter Sequence file: RDB18337_B0Gfe.seq
>D07105A5_B0Gf_2_PGKpro_F_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCN NNNNNCCTTT CGACCTGCAT CCNTCTAGAT    61 CTCGNAGNCA GCTGAANNCT TACCATNNCC GAGTACAAGC CCACGGTGCG CCTCGCCACC   121 CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC   181 ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC   241 TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG   301 GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC   361 CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC   421 CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC   481 GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG   541 CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC ACAACCTCCC CTTCTACGAG   601 CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC   661 ATGACCCGCA AGCCCGGTGC CTGACGCCCG CCCCACGACC CGCAGCGCCC GACCGAAAGG   721 AGCGCACGAC CCCATGCATC GATGATATCA GATCCCCGGG ATGCAGAAAT TGATGATCTA   781 TTAAACAATA AANNATGTCC ACTAAAATGG AAGTTTTTCC TGTCATACTT TGTTAAGAAN   841 GGTGANANNA GAGTANCTAC NTTTTGNATG GAAGGATTGG ANCNNNNGGG NTGGGGGTGG   901 GGTGGGANTA GANNAANNGN CTGNTCNNNN NNGANNNNNN NNTANNNNNN GCNNNANNNA 961 NNNNGNNNCA TANNNGGNNN NNNTANNTTA ANNNNNNNNN N //
Primer: mPGKter_F (Pr0894) Region: mouse PGK ter, loxP, AVVS1 arm2 Sequence file: RDB18337_B0Gff.seq
>D07105A5_B0Gf_2_mPGKter_F_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NTTTGNNNNG TTCTAATTCN NTCAGAAGCT GGTCGAGATC    61 CGGAACCCTT AATATAACTT CGTATAATGT ATGCTATACG AAGTTATTAG GTCCCTCGAA   121 GAGGTTCACT AAGCTTTACT AGGGACAGGA TTGGTGACAG AAAAGCCCCA TCCTTAGGCC   181 TCCTCCTTCC TAGTCTCCTG ATATTGGGTC TAACCCCCAC CTCCTGTTAG GCAGATTCCT   241 TATCTGGTGA CACACCCCCA TTTCCTGGAG CCATCTCTCT CCTTGCCAGA ACCTCTAAGG   301 TTTGCTTACG ATGGAGCCAG AGAGGATCCT GGGAGGGAGA GCTTGGCAGG GGGTGGGAGG   361 GAAGGGGGGG ATGCGTGACC TGCCCGGTTC TCAGTGGCCA CCCTGCGCTA CCCTCTCCCA   421 GAACCTGAGC TGCTCTGACG CGGCTGTCTG GTGCGTTTCA CTGATCCTGG TGCTGCAGCT   481 TCCTTACACT TCCCAAGAGG AGAAGCAGTT TGGAAAAACA AAATCAGAAT AAGTTGGTCC   541 TGAGTTCTAA CTTTGGCTCT TCACCTTTCT AGTCCCCAAT TTATATTGTT CCTCCGTGCG   601 TCAGTTTTAC CTGTGAGATA AGGCCAGTAG CCAGCCCCGT CCTGGCAGGG CTGTGGTGAG   661 GAGGGGGGTG TCCGTGTGGA AAACTCCCTT TGTGAGAATG GTGCGTCCTA GGTGTTCACC   721 AGGTCGTGGC CGCCTCTACT CCCTTTCTCT TTCTCCATCC TTCTTTCCTT AAAGAGTCCC   781 CAGTGCTATC TGGGACATAT TCCTCCGCCC AGAGCAGGGT CCCGCTTCCC TAANGNCCTG   841 CTCTGGGCTT CTGGGTTTGA GTCCTTGGCA AGCCCANGAN ANGCGCTCAN GCTTCCCTGT   901 CCCCCTTCCT CGNCCACCAT CTCATGNCCC TGGNTCTCCT GNCCCTTCCC TANAGGGGTT   961 CCNGGNTCTG NTCNNANNGN CGAANTCNCG GNCNNCTAAN NTNNNTTCGN CNTATANNGA 1021 GNCNNANNAN NNNTNNNCNN GNNCNNNNNN TTNNNNNNNN NN //
Primer: ccdB-C_F (Pr0136) Region: ccdB, NeoR_KanR Sequence file: RDB18337_B0Gfg.seq
>D07105A5_B0Gf_2_ccdB-C_F_B03_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN TCNNNNNNNA GCTGGCGCAT GNNGNCCNCC GNNTATGGCN    61 NNGTGTGCCG GTCTCCGTTA TCGGGGAAGA AGTGGCTGAT CTCAGCCACC GCGAAAATGA   121 CATCAAAAAC GCCATTAACC TGATGTTCTG GGGAATATAA ATGTCAGGCA TGAGATTATC   181 AAAAAGGATC TTCACCTAGA TCCTTTTCAC GTAGAAAGCC AGTCCGCAGA AACGGTGCTG   241 ACCCCGGATG AATGTCAGCT ACTGGGCTAT CTGGACAAGG GAAAACGCAA GCGCAAAGAG   301 AAAGCAGGTA GCTTGCAGTG GGCTTACATG GCGATAGCTA GACTGGGCGG TTTTATGGAC   361 AGCAAGCGAA CCGGAATTGC CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA AGCCCTGCAA   421 AGTAAACTGG ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT CAAGCTCTGA   481 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC   541 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG   601 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC   661 CGACCTGTCC GGTGCCCTGA ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC   721 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG   781 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA   841 GAAAGTATCC ATCATGGCTG ATGCNATGCG GCGGCTGCAT ACGCTTGATC CGGCTACNTG   901 CCCATTCNAC CACCAAGCGA AACATCNCAT CNAGCGNNCN CGTACTCNGN NGGAANCCNN   961 NCTNGNCNAT CNNNATGNNN NGGNNNANNN NCATCNNNGN NNCNCNNNNN NNNNANCTNN 1021 NNNNCNNNNN NNNANNNNNN NNNN //
Primer: ccdB-C_F (Pr0136) Region: ccdB, NeoR_KanR Sequence file: RDB18337_B0Gfg.seq
>D07105A5_B0Gf_2_ccdB-C_F_B03_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN TCNNNNNNNA GCTGGCGCAT GNNGNCCNCC GNNTATGGCN    61 NNGTGTGCCG GTCTCCGTTA TCGGGGAAGA AGTGGCTGAT CTCAGCCACC GCGAAAATGA   121 CATCAAAAAC GCCATTAACC TGATGTTCTG GGGAATATAA ATGTCAGGCA TGAGATTATC   181 AAAAAGGATC TTCACCTAGA TCCTTTTCAC GTAGAAAGCC AGTCCGCAGA AACGGTGCTG   241 ACCCCGGATG AATGTCAGCT ACTGGGCTAT CTGGACAAGG GAAAACGCAA GCGCAAAGAG   301 AAAGCAGGTA GCTTGCAGTG GGCTTACATG GCGATAGCTA GACTGGGCGG TTTTATGGAC   361 AGCAAGCGAA CCGGAATTGC CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA AGCCCTGCAA   421 AGTAAACTGG ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT CAAGCTCTGA   481 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC   541 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG   601 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC   661 CGACCTGTCC GGTGCCCTGA ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC   721 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG   781 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA   841 GAAAGTATCC ATCATGGCTG ATGCNATGCG GCGGCTGCAT ACGCTTGATC CGGCTACNTG   901 CCCATTCNAC CACCAAGCGA AACATCNCAT CNAGCGNNCN CGTACTCNGN NGGAANCCNN   961 NCTNGNCNAT CNNNATGNNN NGGNNNANNN NCATCNNNGN NNCNCNNNNN NNNNANCTNN 1021 NNNNCNNNNN NNNANNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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