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SRAI_pcDNA3

Expression vector of fluorescent protein, SRAI (signal-retaining autophagy indicator) for autophagy sensor.

Catalog number RDB18222
Resource name SRAI_pcDNA3
Alternative name pcDNA3/SRAI
Clone info. Expression vector of fluorescent protein, SRAI (signal-retaining autophagy indicator) for autophagy sensor. SRAI is a tandem fusion of YPet and TOLLES, fused with a DNA linker that encodes a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser.
Comment The accession number in the DDBJ/EMBL/GenBank databases is [LC491575]. Ypet was created by introducing mutations into EYFP. TOLLES was cloned by introducing mutations into monomeric Azami Green (MBL). Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells)
Gene/insert name Aequorea victoria GFP cDNA Galaxea fascicularis GFP cDNA
Reference of insert sequence LC491575 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Katayama, Hiroyuki |
Other clones in our bank

External Database
Aequorea victoria GFP
    Galaxea fascicularis GFP

            Reference sequence
              
             
            Sequence (partial) RDB18222pka01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama H. et al. Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. (2020). Cell 181(5), 1176-1187.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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            MTA, for use for not-for-profit academic purpose [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama H. et al. Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. (2020). Cell 181(5), 1176–1187.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18222 SRAI_pcDNA3 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The SRAI_pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18222).

            Reference section:

            Katayama, H., Hama, H., Nagasawa, K., Kurokawa, H., Sugiyama, M., Ando, R., Funata, M., Yoshida, N., Homma, M., Nishimura, T., Takahashi, M., Ishida, Y., Hioki, H., Tsujihata, Y., Miyawaki, A., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Mitophagy visualization fluorescent sensor, mito-SRAI (English text)
            Featured content マイトファジー可視化蛍光センサー mito-SRAI (Japanese text)
            Featured content Mitophagy (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18222_B0Ffp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward (Pr0016)
            Region: insert 5'
            Sequence file: RDB18222_B0Ffa.seq check
            >D07201A1_B0Ef_1_CMV_Forward_A01_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN TGNNGCAAAT GGGCGGTANG CGTGTACGGT GGGAGGTCTA
               61 TATAAGCAGA GCTCTCTNGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
              121 TACGACTCAC TATAGGGAGA CCCAAGCTTG GTACCGAGCT CGGATCCCAC CATGGTGAGC
              181 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
              241 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
              301 ACCCTGAAGC TGCTCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
              361 ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC
              421 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
              481 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
              541 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
              601 TACAACTACA ACAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG
              661 GCCAACTTCA AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGANCACTAC
              721 CAGCAGAACA CCCCCATCNG CGACGGCCCC GTGCTGCTGC CCGANNACCA CTACCTGANC
              781 TACCAGTCCG CCCTGTTCAA AGACCCCAAC GANAANCGCG NTCACATGGT CCTGCTGGAN
              841 TNCCTGANCG CCGCCGGGAT CACTGAANGN ATGAANNGAN CTGNANNAAN GANCTCNGNN
              901 GGANGNNNTN NNNNNNNNGT GGCTCTNGNG GNGNNNNATC GNTCNNNATG GNNANCNNGN
            961 TNNANNNNNN NNNN
            //
            Primer: f1ori_R3 (Pr0142)
            Region: BGH p(A), insert 3'
            Sequence file: RDB18222_B0Ffb.seq check
            >D07201A1_B0Ef_1_f1ori_R3_B05_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TCTTTCCGCC TCNNAANCCN TAGAGCCCAC
               61 CGCATCCCCA GCATGCCTGC TATTGTCTTC CCAATCCTCC CCCTTGCTGT CCTGCCCCAC
              121 CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC GATGCAATTT CCTCATTTTA
              181 TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG GAAGGCACGG GGGAGGGGCA
              241 AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT GATCAGCGAG CTCTAGCATT
              301 TAGGTGACAC TATAGAATAG GGCCCTCTAG ATGCATGCTC GAGCGGCCGC CAGTGTGATG
              361 GATATCTGCA GAATTCGCTA GCTCACTTGG CCTGGCTGGG CAGCATGGAG GCCCTGGCCA
              421 CGGCGTTCTC GCACAGCTTC ACCTTGTTGT AGTCCTTGTC GTGCTCCAGG ATCTCGATCC
              481 TGTGGTCGAT CTTGTGGGCG TCGGGCAGGC TCACCTCCTT CTTGGCCTTG TAGGTGGTCT
              541 TGACGTCGCA CCTGTAGTGG CCGCCGCCCT CCAGCAGCAG CCTCATGTTC ACGTCGCCCT
              601 TCAGCACGCC GTCCTCCACG TACATCTTCT CGGTGTCGGG CTCCCACTTC AGGGTCTTCT
              661 TCTGCATCAC GGGGCCGTTG GGGGGGAAGT TGGTGCCGTC GAACCTGATG TCGTANAANA
              721 ANCAGTCGCC CCTCATGCTG ATGTTGCTGG TGGCGGTGCA GATGCCCTGG TCCTCGTANG
              781 TCATGCTCCT CTCCCAGTGG TAGCCCTCGG GGAANGNCTG CTTGAAGTAN TCCTGGATGT
              841 CGGCGGGGNN NTTGGNGAAN GNCNNGTTGC CGTACTGGAN CNANGNGGAN AGGATGTCGN
              901 NNNNNAANGG CAGGGGGGCN NCNTCNGNCA CGNTTCNNNT NCNNGNNCTG GGNGNCNNNN
            961 NNNGGGNTNG CCCTNNCCNN CNNCCNNNNN NCACNNANNN TNNNNNNNNN N
            //
            Primer: mVenus-M_F (Pr0574)
            Region: insert mid
            Sequence file: RDB18222_B0Ffc.seq check
            >D07201A1_B0Ff_1_mVenus-M_F_C01_07_ABI24.ab1
                1 NNNNNNNNNN NNANNNANGN NNTCNNGGCC NACTTCAAGA NCCGCCNCAA CATCGAGGAC
               61 GGCGGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG
              121 CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCGCCC TGTTCAAAGA CCCCAACGAG
              181 AAGCGCGATC ACATGGTCCT GCTGGAGTTC CTGACCGCCG CCGGGATCAC TGAAGGCATG
              241 AACGAGCTGT ACAAGGAGCT CGGTGGAGGC GGTTCAGGCG GAGGTGGCTC TGGCGGTGGC
              301 GGATCGCTCG AGATGGTGAG CGTGATCAAG CCCGAGATGA AGATCAAGCT GTGCATGAGG
              361 GGCACCGTGA ACGGCCACAA CTTCGTGATT GAGGGCGAGG GCAAGGGCAA CCCCTACGAG
              421 GGCACCCAGA TCCTGGACCT GAACGTGACC GAGGGCGCCC CCCTGCCCTT CGCCTACGAC
              481 ATCCTGTCCA CCTCGTTCCA GTACGGCAAC AGGGCCTTCA CCAAGTACCC CGCCGACATC
              541 CAGGACTACT TCAAGCAGGC CTTCCCCGAG GGCTACCACT GGGAGAGGAG CATGACCTAC
              601 GAGGACCAGG GCATCTGCAC CGCCACCAGC AACATCAGCA TGAGGGGCGA CTGCTTCTTC
              661 TACGACATCA GGTTCGACGG CACCNACTTC CCCCCCAACG GCCCCGTGAT GCAGAAGAAG
              721 ANCCTGAAGT GGGAGCCCGA CACCGANAAG ATGTACGTGG AGGANGGCGT GCTGAAGGGC
              781 GACGTGAACA TGAGGCTGCT GCTGGANGGC GGCGGCCACT ACAGGTGCNA CGTCANNACC
              841 ACCTACNNNN CCNAGAANGA NGTGAGCCTG CCCNANNNCC ACAAGATCGA CCACAGGATN
              901 NANATNCTGG ANCACGANNN NGNNTANNNC NAGGNGANNC TGNNCNANAA CNCCGNNNNN
              961 NNGGGNNTNN NNGCNNCCNN NCCNGNNNNA NNNNNNCNNN NNNNNTNNNN NNNANNNNNN
            1021 NNNN
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40pro_ori, NeoR
            Sequence file: RDB18222_B0Ffd.seq check
            >D07201A1_B0Ff_1_SV40pro_ori_D01_10_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNG NNNNNNTCTG CCTCTGANCT ATTCNNGAAG TAGTGAGGAG
               61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
              121 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
              181 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
              241 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
              301 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
              361 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
              421 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
              481 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
              541 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
              601 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
              661 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA NGNTCTCGTC GTGACCCATG
              721 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
              781 GTGGCCGGCT GGGTGTGGCG GANCGCTATC ANGACATANC GTTGGCTACC CGNGATATTG
              841 CTGAAGANCT NGGCGGCNAA NGGGCTGANC GCTTCCNCNN GCTTTACNGT ATCGCCNCTC
              901 CNGANNNNCA NCNNANCGCC TTCNNTCNNC NNNNNNNNNA NNNNNNCNNA NCNGGNNTCT
            961 GGGGGNTNCN NANNNNNNNN NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. [link to RRC of NBRP]

            2024.03.26

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