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pmRegnase-1-(130-300)-D141N-L2-mCherryX2-C1

Expression vector of mouse zinc finger protein Regnase-1 (Zc3h12a), 130 - 300 a.a truncated form with a mutation reducing RNase activity tagged with 2 x mCherry with a linker.

Catalog number RDB18154
Resource name pmRegnase-1-(130-300)-D141N-L2-mCherryX2-C1
Clone info. Expression vector of mouse zinc finger CCCH type containing 12A (Zc3h12a; a.k.a. Regnase-1), 130 - 300 a.a truncated form with D141N mutation lacking RNase activity and (GGGGS) x 4 linker (L2), tagged with 2 x mCherry at C-erminus, CMV promoter, see Fig. 3F of Mino, T. et al., Nucleic Acids Res., 47 (16): 8838-8859, 2019.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pmCherry-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Zc3h12a cDNA
Depositor|Developer Takeuchi, Osamu | Mino, Takashi |
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External Database
mouse Zc3h12a
          Reference sequence
            
           
          Sequence (full) RDB18154zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Mino, T. et al., Nucleic Acids Res. 47 (16), 8838-8859, 2019) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
          For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
          RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mino, T. et al., Nucleic Acids Res. 47 (16), 8838-8859, 2019) 。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
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          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB18154 pmRegnase-1-(130-300)-D141N-L2-mCherryX2-C1 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pmRegnase-1-(130-300)-D141N-L2-mCherryX2-C1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18154).

          Reference section:

          Mino, T., Iwai, N., Endo, M., Inoue, K., Akaki, K., Hia, F., Uehata, T., Emura, T., Hidaka, K., Suzuki, Y., Standley, D.M., Okada-Hatakeyama, M., Ohno, S., Sugiyama, H., Yamashita, A., Takeuchi, O., Translation-dependent unwinding of stem-loops by UPF1 licenses Regnase-1 to degrade inflammatory mRNAs. Nucleic Acids Res. 47 (16): 8838-8859 (2019). PMID 31329944. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Mino, T., Translation-dependent unwinding of stem-loops by UPF1 licenses Regnase-1 to degrade inflammatory mRNAs. Nucleic Acids Res. 47 (16): 8838-8859 (2019). PMID 31329944. [link to RRC of NBRP]

          2023.12.13

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