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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET28-3C-eIF2Ba

Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit alpha (eIF2Balpha).

Catalog number RDB17722
Resource name pET28-3C-eIF2Ba
Alternative name eIF2Balpha
Clone info. Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit alpha (eIF2Balpha) cloned into the pET-28c, in which the thrombin cleavage site was replaced by the HRV 3C protease cleavage site.
Comment Expression was confirmed by the depositor.
Vector backbone pET28c (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kanamycin
Gene/insert name human EIF2B1 cDNA
Depositor|Developer Ito, Takuhiro |
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External Database
human EIF2B1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。
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          RDB17722 pET28-3C-eIF2Ba DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17722_B0Adp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: lacIpro_R1
          Region: lacO, RBS, 6xHis, HRV 3C site, insert 5'
          Sequence file: RDB17722_B0Ada.seq check
          >D06721A1_B0Ad_3_lacIpro_R1_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNAN NNNGCGCTCA TGANCCCGAA GTGGCGAGCC
             61 CGATCTTCCC CATCGGTGAT GTCNGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCN
            121 GNGNNGNCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAATTAA
            181 TACGACTCAC TATAGGGGAN TTGNGAGCGG ATAACAATTC CCCTCTAGAA ATAATTTTGT
            241 TTAACTTTAA GAAGGAGATA TACCATGGGC AGCAGCCATC ATCATCATCA TCACAGCAGC
            301 GGCCTTGAAG TCCTCTTTCA GGGACCCGGG CATATGGACG ACAAGGAGTT AATTGAATAC
            361 TTTAAGTCTC AGATGAAAGA AGATCCTGAC ATGGCCTCAG CAGTGGCTGC CATCCGGACG
            421 TTGCTGGAGT TCTTGAAGAG AGATAAAGGG GAGACAATCC AGGGTCTGAG GGCGAATCTC
            481 ACCAGTGCCA TAGAAACCCT GTGTGGTGTG GACTCCTCTG TGGCAGTGTC CTCTGGCGGG
            541 GAGCTCTTCC TCCGCTTCAT CAGTCTTGCC TCCCTGGAAT ACTCCGATTA CTCCAAATGT
            601 AAAAAGATCA TGATTGAGCG GGGAGAACTT TTTCTCAGGA GAATATCACT GTCAAGAAAC
            661 AAAATTGCAG ATCTGTGCCA TACTTTCATC AAAGATGGAG CGACAATATT GACTCACGCC
            721 TACTCCAGAG TGGTCCTGAN AGTCCTGGAA NNCAGCNNNN NCGGNCAAGA NGCGATTTAG
            781 TGTATACGTC ACAGAGTCAC ANCCTGATTT GTCAGGNAAG AAAATGGCCA AAGCCNNCNN
            841 NCACCTCAAC GTNCCTGNCA CTGTGNTGCT AGATGCTGCT GTCNGGCTAC NTNNNNGAGA
            901 AAGCAGATCT TGNCATANNT NNGTGCTGAN NGAGTTGTTN AAAACNGNNN GNATTNNTNA
          961 CANNNNNNGA NNNNNNNNNN
          //
          Primer: T7 terminator
          Region: insert 3'
          Sequence file: RDB17722_B0Adb.seq check
          >D06721A1_B0Ad_3_T7 terminator_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGNNGNTTN NNNNNCNNNC CGGNTCTCNG TGGNNNGTGG
             61 TGGTGGTGCT CGAGTGGGCC GCTCACAGAT AGAGCTTGAT GAGCTCATCG CTGACTGCTG
            121 AGGGTGTCAG CACGCCCAGG TCTGTAAACA GCAGAGTGAT TAAGGAAGGG GCAGTGTAGT
            181 CGACCCACGG ATGCTCCTCT TTGAGGTCTT GTCCAGTCTG CGCGACCTTG AGAGTGTCTG
            241 CCTTATACTT AAACTTATCT GGGACGTCTT GCTGGTTTAG TGGAAAGAGC CGGACAAACT
            301 TGAAACTTTC TGCAACCACA TAGAAAGGTT TGTTCTGTGC TTTGGCACAC ACAGCCATCT
            361 GGTTGGTTCC AATCTTGTTA ATAATTCCTC CGTTTTCAAC AACTCCTTCA GCACCAACTA
            421 TGACAAGATC TGCTTTCTCC ATGATGTAGC CGACAGCAGC ATCTAGCACC ACAGTGACAG
            481 GGACGTTGAG GTGGCAGAGG GCTTTGGCCA TTTTCTTACC TGACAAATCA GGCTGTGACT
            541 CTGTGACGTA TACACTAAAT CGCTTCTTGG CCGCCACGGC TGCTTCCAGG ACTCTCAGGA
            601 CCACTCTGGA GTAGGCGTGA GTCAATATTG TCGCTCCATC TTTGATGAAA GTATGGCACA
            661 GATCTGCAAT TTTGTTTCTT GACAGTGATA TTCTCCTGAG AAAAAGTTCT CCCCGCTCAA
            721 TCATGATCTT TTTACATTTG GAGTNANNCG GANTATTCCA GGGAGGCAAG ANTGATGAAG
            781 CGGANGAAGA NCTCCCCGCC AGNNGACACT GCCACAGANG ANTCCACACC ACACAGGGTT
            841 TCTATGGCAC TGGTGANANT NNNNCCNCAN ANCCNGNNNT GTCTCCCCTT NATCTCNCTT
            901 CNAGANNTNC NNNNNNNNTC CNGNTGGCAG CCNCNGNNTN ANNNNNTGTC NGGATCTTCT
            961 TTCNTCTGAN ANTTAAANNN ANTNCNANTN NNTNCNTNNN NNGNNCNNTA TNNCCNNGNN
          1021 NNCCNNNNNN NNNGNNNNTN NNNNNNNNN
          //
          Primer: pET_lacIpro_F2
          Region: lacI pro, lacI
          Sequence file: RDB17722_B0Adc.seq check
          >D06721A1_B0Ad_3_pET_lacIpro_F2_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNCNNTNG CTGGCGCCTA TATCGCCGAC ATCACCGATG
             61 GGGAAGATCG GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG
            121 CAGGCCCCGT GGCCGGGGGA NTGTTGGGCG CCATCTCCTT GCATGCACCA TTCCTTGCGG
            181 CGGCGGTGCT CAACGGCCTC AACCTACTAC TGGGCTGCTT CCTAATGCAG GAGTCGCATA
            241 AGGGAGAGCG TCGAGATCCC GGACACCATC GAATGGCGCA AAACCTTTCG CGGTATGGCA
            301 TGATAGCGCC CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT AACGTTATAC
            361 GATGTCGCAG AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT GAACCAGGCC
            421 AGCCACGTTT CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA GCTGAATTAC
            481 ATTCCCAACC GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT TGGCGTTGCC
            541 ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA ATCTCGCGCC
            601 GATCAACTGG GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAANNGGCGT CGAAGCCTGT
            661 AAAGCGGCGG TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT TAACTATCCG
            721 CTGGATGACC AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC GGCGTTATTT
            781 CTTGATGTCT CTGACCAGAC ACNCATCNAC AGTATTATTT TCTCCCNTNA AGACNGTACG
            841 CGACTGGGCG TGGNNCATCT GGTCGCATNN GGGTCACCAG CAAAATCGCG CTGTTNGCGG
            901 GCCCNNTAAG TTCTGNNTNN GNNGCGTCTG CNNCNNGNNT GNCTNGCATN AANNNNNNTC
          961 NNTCNNNNNC NAANTNNNGN CNATANCNGN NANNGGNNAN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl