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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCRT

Plasmid vector for TA-cloning of PCR-products.

Catalog number RDB17479
Resource name pCRT
Clone info. Plasmid vector for TA-cloning of PCR-products. Map and cloning site region, see Fig. 1 of Motohashi, K. Sci. Rep. 9 (1): 6417, 2019.
Vector backbone pUC18 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Depositor|Developer Motohashi, Ken |
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External Database
            Reference sequence
              
             
            Remarks, protocol and/or map (png) RDB17479z.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) and an acknowledgment to the DEPOSITOR are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Motohashi, K. Sci. Rep. 9(1): 6417, 2019) を引用し、謝辞の表明を必要とする。学術機関の学術研究に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB17479 pCRT DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pCRT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17479).

            Reference section:

            Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            Featured content A series of high-efficiency PCR-cloning vectors and their useful application [link] (English text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)
            Featured content A series of high-efficiency PCR-cloning vectors and their useful application [link] (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17479_A9Hqp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-CAPbs_up_F (Pr0536)
            Region: CAP site, lac pro, lac op, lacZ alpha(Xcml-NheI-XcmI)
            Sequence file: RDB17479_A9Hqa.seq check
            >D06545A1_A9Hq_3_pUC-CAPbs_up_F_A12_03_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNCNNNNNC AATTAATGTG AGTTAGCTCA CTCATTAGGC
               61 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
              121 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGAGCTCG GTACCCCAAT
              181 ACTTGTATGG AGACGCTAGC GTCTCCATAC AAGTATTGGG GGATCCTCTA GAGTCGACCT
              241 GCAGGCATGC AAGCTTGGCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG
              301 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG
              361 AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCC
              421 TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC
              481 TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG
              541 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG
              601 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA
              661 AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA
              721 CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA
              781 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG NTAAATGCTT CAATAATATT
              841 GAAAAAGGAA GAGTATGANT ATTCNACATT TCCGTGTCGC CCNTATTCCC NTTTTTNGNG
              901 GCATTTNGCC TTNCNGNTTT TTGCTCACCC AGAANNGCTN GNGAANNTAA AAGANGCTGA
            961 AGANCAGNTN NGNNGCNNNN NNGGNNNNNN TCGNANNNNN NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Motohashi, K., A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417 (2019). PMID 31015513.

            2023.05.22

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