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pBAD33betaH

Bacterial expression vector of beta-subunit of Q-beta replicase.

Catalog number RDB17458
Resource name pBAD33betaH
Clone info. Bacterial expression vector of beta-subunit of Q-beta replicase tagged with His at C-terminus.
Comment The plasmids pBAD33 was kindly provided by Dr. S. Yasuda of the National Institute of Genetics, Japan. The plasmid ZpQbeta32 containing cDNA of the Qbeta phage genome was kindly provided by Dr. Y. Inokuchi. Expression was confirmed by the depositor with SDS-PAGE and CBB staining.
Vector backbone pBAD33 (plasmid)
Selectable markers Chloramphenicol
Gene/insert name Bacteriophage Qbeta DNA polymerase genomic
Depositor|Developer Ichihashi, Norikazu |
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External Database
Bacteriophage Qbeta DNA polymerase
            Reference sequence
              
             
            Sequence (full) RDB17458zzk02.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biosci. Bioeng. 101(5), 421-426, 2006)
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biosci. Bioeng. 101(5), 421-426, 2006)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            RDB17458 pBAD33betaH DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pBAD33betaH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17458).

            Reference section:

            Kita, H., Cho, J., Matsuura, T., Nakaishi, T., Taniguchi, I., Ichikawa, T., Shima, Y., Urabe, I., Yomo, T., Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit. J. Biosci. Bioeng. 101 (5): 421-426 (2006). PMID 16781472.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17458_A9Fop1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: araBADpro_F1 (Pr0654)
            Region: araBAD pro, insert 5'
            Sequence file: RDB17458_A9Foa.seq check
            >D06471A5_A9Fo_1_araBADpro_F1_F05_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCTTTT NNTCGCAACT CTCTACTGTT
               61 TCTCCNTACC CGTTTTTTTG GGCTAGCAGG AGGAATTCCA TATGTCTAAG ACAGCATCTT
              121 CGCGTAACTC TCTCAGCGCA CAATTGCGCC GAGCCGCGAA CACAAGAATT GAGGTTGAAG
              181 GTAACCTCGC ACTTTCCATT GCCAACGATT TACTGTTGGC CTATGGTCAG TCGCCATTTA
              241 ACTCTGAGGC TGAGTGTATT TCATTCAGCC CGAGATTCGA CGGGACCCCG GATGACTTTA
              301 GGATAAATTA TCTTAAAGCC GAGATCATGT CGAAGTATGA CGACTTCAGC CTAGGTATTG
              361 ATACCGAAGC TGTTGCCTGG GAGAAGTTCC TGGCAGCAGA GGCTGAATGT GCTTTAACGA
              421 ACGCTCGTCT CTATAGGCCT GACTACAGTG AGGATTTCAA TTTCTCACTG GGCGAGTCAT
              481 GTATACACAT GGCTCGTAGA AAAATAGCCA AGCTAATAGG AGATGTTCCG TCCGTTGAGG
              541 GTATGTTGCG TCACTGCCGA TTTTCTGGCG GTGCTACAAC AACGAATAAC CGTTCGTACG
              601 GTCATCCGTC CTTCAAGTTT GCGCTTCCGC AAGCGTGTAC GCCTCGGGCT TTGAAGTATG
              661 TTTTAGCTCT CAGAGCTTCT ACACATTTCG ATATCAGAAT TTCTGATATT AGCCCTTTTA
              721 ATAAAGCAGT TACTGTACCT AAGAACAGTA AGACAGATCG TTGTATTGCT ATCGAACCTG
              781 GTTGGAATAT GTTTTTCCNA CTNGGTATCG GTGGCATTCT ACGCGATCGG NTGCGTTGCT
              841 GGGGNTATCG ATCTGAATGA TCAGACGATA AATCAGCGCC GCGCTCACGA NNGNNNCNNT
              901 ACNNNTAACN TANCAANNGN NGATCTNNCA NNNGNNNGCN ATTCNATATC NCTNNNCNNN
            961 NNNNNNN
            //
            Primer: rrnBT1ter_F2 (Pr0531)
            Region: rrnBT1 ter, 6xHis, insert 3'
            Sequence file: RDB17458_A9Fob.seq check
            >D06471A5_A9Fo_1_rrnB1ter_F2_H05_23_ABI24.ab1
                1 NNNNNNNNNN TNNNNNNNNN NCTTTCGTTT NNTTTGATGC CTGGCAGTTC CCTACTCTCG
               61 CATGGGGAGA CCCCACACTA CCATCGGCGC TACGGCGTTT CACTTCTGAG TTCGGCATGG
              121 GGTCAGGTGG GACCACCGCG CTACTGCCGC CAGGCAAATT CTGTTTTATC AGACCGCTTC
              181 TGCGTTCTGA TTTAATCTGT ATCAGGCTGA AAATCTTCTC TCATCCGCCA AAACAGCCAA
              241 GCTTCGTAAA TTCTATACAA AATTAGTGGT GGTGGTGGTG GTGAGATCTC GCCTCGTGTA
              301 GAGACGCAAC CTTCGTGCCC TGGAAGACCC CGTAGGGTGC CAGAACACGG CTACTGCACG
              361 CGATATACTG TATATCGAAT TTGCCGGTAG ACCTGCTTAT CTTCGTAGGA TTACTCCTAC
              421 AGATAAGCTG ATCGATGGCA AATAGATCCG CAGAATCGCA ACCCGATGGA CCCCTAAGAG
              481 GCAACCCATC GTTACTTTCC GAGAGACAAC GCGAGAAGAG GTCGTAGAGA TACGACCCCA
              541 ACTCAGCGCG CTCTCGGTCC CTTGTATGGT CCGTAATCAC CGGTACGTAC CGGATCCACC
              601 CGCGGTTTTT CGCGAAAGGA TTGATTAGGA CCGATCCGAC GAGGGCACCA TCACCGTAAC
              661 CATCAGGTAT AGTATTACGT TGCAGCTGTT TAGGCAGCAA CTTACGATAC TTGAGGTACA
              721 CAGAATGGGC CCTANGATCC CATACGCCGT CAATTGTGGC CCACCGATAT AGGTTATTCA
              781 AAACCAGTAT TAAATCGGCA GGACTCACTA TACGGTGACG TATGTAAAAG GGAGTAACAT
              841 CTACGCCNGA NTAGTAGTGC TTGCCGCACG ACTCTCTGAA CGGCCCCTCG GAAAAAGNCT
              901 TTTTTAGTAT TGGNCGNNAA NNNNACNTAC TTNAAANNTT CCNNGNANGN NAGGNNNTGC
            961 NNNNGNANNG NAAANTNNTA TCGNNNNNNN NANNNNNNNN NN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Kita, H., Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit. J. Biosci. Bioeng. 101 (5): 421-426 (2006). PMID 16781472.

            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl