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p3XFLAG-HELLS WT

Expression vector of human HELLS wild-type.

Catalog number RDB17376
Resource name p3XFLAG-HELLS WT
Alternative name p3XFLAG-CMV10-HELLS WT
Clone info. Expression vector of human helicase lymphoid specific (HELLS) wild-type, tagged with 3 x FLAG at N-terminus. Structure, see Fig. S1 of Unoki, M. et al., J. Clin. Invest., 129 (1): 78-92, 2019.
Comment Expression was confirmed by the depositor with western blottingand RT-PCR.
Vector backbone p3xFLAG-CMV-10 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human HELLS cDNA
Depositor|Developer Unoki, Motoko |
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External Database
human HELLS

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。
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          RDB17376 p3XFLAG-HELLS WT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17376_A9Fhp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Region: CMV pro, 3xFLAG, insert 5'
          Sequence file: RDB17376_A9Fha.seq check
          >D06399A3_A9Fh_1_CMV_Forward_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNCNCN NNNNNNNNNN NGGGCGGTAG GCGTGNNNNG TGGGAGGTCT
             61 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGAATTAACC ATGGACTACA AAGACCATGA
            121 CGGTGATTAT AAAGATCATG ACATCGATTA CAAGGATGAC GATGACAAGC TTGCGGCCGC
            181 GATGCCAGCG GAACGGCCCG CGGGCAGCGG CGGCTCGGAG GCTCCAGCAA TGGTTGAACA
            241 ACTGGACACT GCTGTGATTA CCCCGGCCAT GCTAGAAGAG GAAGAACAGC TTGAAGCTGC
            301 TGGACTAGAG AGAGAGCGGA AGATGCTGGA AAAGGCTCGC ATGTCTTGGG ATAGAGAGTC
            361 GACAGAAATT CGGTACCGTA GACTTCAACA TTTGCTTGAA AAAAGCAATA TCTACTCCAA
            421 ATTTTTATTG ACGAAAATGG AACAGCAACA ATTAGAGGAA CAGAAGAAGA AAGAAAAATT
            481 GGAGAGAAAA AAGGAGTCTT TAAAAGTTAA AAAGGGTAAA AATTCAATTG ATGCAAGTGA
            541 AGAGAAGCCA GTTATGAGGA AAAAAAGAGG AAGAGAAGAT GAATCATACA ATATTTCANA
            601 GGTCATGTCA AAAGAGGAAA TTTTGTCTGT GGCTAAAAAA AATAAAAAGG AGAATGAGGA
            661 TGAAAACTCN TNNTCTACTA ATCTCTGTGT GGAAGATCTT CANAAAAATA AAGATTCNAA
            721 TAGTATAATT AAAGATAGAT TGTCTGAAAC GGTTAGGCAG AATACTAAAT TCTTTTTTGA
            781 NNNNNTCCGG AAGTGTAATG NTCAGNCNNT ACCTTTTCNA CNACCNAAGC ACTTCACTGG
            841 AGGANTGATG CGATGGTANN NAGTANAANG NNTGNNATGG NTNNNNNNGC TTTGGGAAAA
            901 TGNAATTAAT GGCATTTTTA NCNNATNAAN TGGGNNTNGG NNNNNANNNN NCNNNGCNTN
          961 GCTNNNNNNN NNN
          //
          Primer: hGHpolyA-R
          Region: hGHp(A), insert 3'
          Sequence file: RDB17376_A9Fhb.seq check
          >D06399A3_A9Fh_1_hGHpolyA-R_E01_05_ABI08.ab1
              1 CNNNNNNNNN NNNNNNNNNN NANANTANAG NAGGANACCT AGTCNNNACA AAATGATGCA
             61 ACTTAATTTT ATTAGGACAA GGCTGGTGGG CACTGGAGTG GCAACTTCCA GGGCCAGGAG
            121 AGGCACTGGG GAGGGGTCAC AGGGATGCCA CCCGGGATCC TCTAGAGTCG ACTGGTACCG
            181 ATATCAGATC TATCGATGAA TTCGCGGCCG CTTAAAACAA ACATTCAGGA CTGGAATCTT
            241 CAGAATTTTC TAATATCTTG AATATCCCCA TCTTCTCTTT AATTGGTCCT GAAGCATTCA
            301 TTTGATCAAT AAGATCACTT CGATCTAACA ACAACTCTAG ATCTTTATCA CTAATGACCT
            361 TCTCTCTTGA TCCTTTTATT TCCCTTTCAT AATCTCTAGA TTTTAATAAT TCCATTAATT
            421 CCTTAGGATC TAAGAAATTC TTAGACAGAT TTAATCCAGA CTGACCACCT TTGAAATGAT
            481 TTTTATGGAT GATCAACTTT TCCAGTTTCC TTTTAGCAGC TGCTCTTTCC ACAATTTTCT
            541 GATCGATAGT ATTTGCTGTA ACAAGGCGAT AAACAACAAC TGGCTTTGTC TGACCAATTC
            601 TATGACATCT ATCCTGGGCC TGAAGATCCG ACTGGGGGTT CCAATCACTA TCATAAATGA
            661 TAACTGTATC TGCTGCAGTC AGATTAATGC CCAGGCCACC AGCTCGTGTA CTCNCTAAGA
            721 AGANAAACAC CTCTGGATCC GTGTTGAAGC TGTGCATGTT TTTTTCTCTC TCTGANTAAG
            781 ACATGGACCC NTCAAGCCTG CTGAAGTTGA AATCTCTGAG ANGGCAGTAA TCCNTCNAAA
            841 TGTCCNANAT GCTTGTCATT TGTGAAAAAA GCANCNCCNT GNGANNTCTT TTTTTTANTT
            901 CTNGNCANNN TTCNATNCNA AATCNNNAAN NTNCNNGAAT TTGNNANNNN TTCNTCNTCN
          961 ATNNNNNNNC NNNNGTNNNN NGNNNNANNN NNNANNNNNN NNNN
          //
          Primer: SV40pro_F
          Region: NeoR/KanR
          Sequence file: RDB17376_A9Fhc.seq check
          >D06399A3_A9Fh_1_SV40pro_F_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGNNGNTT TTTTGGANGA NNTGATCNGC
             61 TTGGGANCTG ATCAAGAGAC AGGNTGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
            121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
            181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
            241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
            301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
            361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
            421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
            481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
            541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
            601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
            661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGANTCNTC
            721 GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCANGACN TAGCGTTGGC TACCCGTGAT
            781 ATTGCTGAAN AGCTTGGCGG NNAATGGGCT GACCGCTTCC TCGTGCTTNA CGGTATCGCC
            841 GNTCNNNATT CNCANCGCAT CNCNNTCTAT CGNCNTNNNG ACNANTNCTT CTGANCGGGA
            901 NTCTNGGGGT TNNAAATGNN NNANNNANNN ACNNCCAACC NNNNNTCNCN ANNNTTCNAN
          961 NNNNNNNGNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl