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pEF-T7-GST-RBD32/38

Expression vector of ANKR1domain of mouse Ankrd27.

Catalog number RDB17371
Resource name pEF-T7-GST-RBD32/38
Clone info. Expression vector of ANKR1domain of mouse ankyrin repeat domain 27 (Ankrd27) tagged with T7-GST at N-terminus, EF1 promoter.
Vector backbone pEF-T7 (plasmid)
Selectable markers Amp^r.
Gene/insert name mouse Ankrd27 cDNA
Reference of insert sequence AB455262 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Ankrd27

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
          Additional terms and conditions:
          Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Marubashi, S. et al. J. Biol. Chem. 291, 1427-1440, 2016); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。
          付加的提供条件:
          pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB17371 pEF-T7-GST-RBD32/38 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEF-T7-GST-RBD32/38 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17371).

          Reference section:

          Marubashi, S., Shimada, H., Fukuda, M., Ohbayashi, N., RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes. J. Biol. Chem. 291 (3): 1427-1440 (2016). PMID 26620560.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17371_A9Fap1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F (Pr0168)
          Region: EF-1 alpha pro,T7 tag,GST 5'
          Sequence file: RDB17371_A9Faa.seq check
          >D06377B6_A9Fa_1_EF1a-C_F_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNTTT NANTTTGGNN CTTGGTTCNT TCTCAAGCCT CAGACAGTGG
             61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGAAT TCTCTAGAGC GGCCGCGGGA
            121 ATTCGATTCC ACCATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGCG GATCTATGTC
            181 CCCTATACTA GGTTATTGGA AAATTAAGGG CCTTGTGCAA CCCACTCGAC TTCTTTTGGA
            241 ATATCTTGAA GAAAAATATG AAGAGCATTT GTATGAGCGC GATGAAGGTG ATAAATGGCG
            301 AAACAAAAAG TTTGAATTGG GTTTGGAGTT TCCCAATCTT CCTTATTATA TTGATGGTGA
            361 TGTTAAATTA ACACAGTCTA TGGCCATCAT ACGTTATATA GCTGACAAGC ACAACATGTT
            421 GGGTGGTTGT CCAAAAGAGC GTGCAGAGAT TTCAATGCTT GAAGGAGCGG TTTTGGATAT
            481 TAGATACGGT GTTTCGAGAA TTGCATATAG TAAAGACTTT GAAACTCTCA AAGTTGATTT
            541 TCTTAGCAAG CTACCTGAAA TGCTGAAAAT GTTCGAAGAT CGTTTATGTC ATAAAACATA
            601 TTTAAATGGT GATCATGTAA CCCATCCTGA CTTCATGTTG TATGACGCTC TTGATGTTGT
            661 TTTATACATG GACCCAATGT GCCTGGATGC GTTCCCAAAA TTAGTTTGTT TTAAAAAACG
            721 TATTGAAGCT ATCCCACAAA TTGATAAGTA CTTGAAATCC AGCAAGTATA TAGCATGGCC
            781 TTTGCANGGC TGGCAAGCCA CGTTTGGTGG TGGCGANCAT CCTCCAAAAT CGGATCTGGT
            841 TCCGCGTGGA TCCGACCCGT CAGTTGTCNC TCCTTTCTCC AGANATGANA GANGTCAAAC
            901 ACCCCTCCAT GTGGCTGCCC TCTGNGGNCA GGCATCCCTC ATTGACTTCC TGGTCTCCNA
            961 ANGNGNNNNA NNNNNTGNNN NANANTATCA NGGGNNCACC CCCNTNANNT NNGGNNNGNN
          1021 ANAANNNNTN NNNANNTGNG ANNCNNCTGN NNNNNNNNN
          //
          Primer: hGCSF_R (Pr0031)
          Region: hG-CSF3' UTR+pA,insert 3'
          Sequence file: RDB17371_A9Fab.seq check
          >D06377B6_A9Fa_1_hGCSF_R_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNAG NNNNNNNGGT GGGGNNCCTC ACTCTAGAGC GGCCGCGAAT
             61 TCACTAGTGA TTTCAAATCC TGGCCAGTTT CTGAGCTGGA GCACACTTGG GACACTGGCA
            121 TAAGGGGTGA CAGAATTCGA GATCCACTGT GCTGATGGCG TCTTCCACAT CGTCTAGGTC
            181 ATCCTCTGTC CACTCCAAAA GGTATCGTAC CATTTCTAGA TCGCCATCAG CAACTGCCCT
            241 TAGAAGCTTT TCTACCTCCC TGTAGTCCTT TTTGACCTCT TCTTGCCTGA AGCTCACCGA
            301 TATGGAAGAA AAGCTGGAGG TGGAGGAGCC CTGGCTGATG GAATCCACAG AGCGTGTAGG
            361 GGACTGTGCA GGGACCTCAG AAGGCCTTGG CCTCCTGTCA GAGGATAGGT GATGAGCTTC
            421 CATGATAGAC AGAATCTTCG AGTTCAGAGC ACACTTGAGT GGTGTTTCCT TCAGTCTGTT
            481 CTGGACTGCT GTGGGGGCTC CATTCTGCAG CAGTGTCTCT ATGATGCCCT CGTAACCCCA
            541 GCGTGCAGCA ATGTGCAGGG CTGTGTCTCC TTTCTCATTA CCAATATCCA ATCTGCAGGC
            601 CTGGACATCA TAGTAGACCA GGGCCTTCAC GCAATCCTCC TGCCCGTAGG TGCAGGCCAG
            661 GTGCAGCGGG GTGTTGCCGT TGTTGTCCTG AACCTCAGTG CTGGCCTTGT AGTGCAGCAG
            721 CAGCAGCGTC ACACTTTGGA AGCCTTTCTG ACATGCCAGA TGTAAGGGGG TGGANCCATG
            781 ATAGTCTGTG GCATTCACTA CGGCACCTTT GGAGACCANG AAGTCNNTGA GGGATGCCTG
            841 ACCNCAGAGG GCAGCCACAT GGNNGGGTGT TTGACCTCTG TCATCTCTGG ANAAANNANT
            901 GACNACTGAN GGNTCGGANN NNCGCNNANN NAGATNCNAT TTTGNANGAN GGNCNNCNCC
            961 NCCNAACNTG GNTTGCNNGC CCTGNNAANG NCATGCTATA TACTTGCNNG NNTNNNAGNN
           1021 NTNNNNNNAN NTNNNGGGNN ANNNTNANNN NNNTTTTTTN AAANNNAANC TNNNTTTNNG
          1081 NNNNNNNNNN
          //
          Primer: pGEX-F2 (Pr0403)
          Region: GST,thrombin site,insert 5'
          Sequence file: RDB17371_A9Fac.seq check
          >D06377B6_A9Fa_1_pGEX-F2_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTA TTGNAGCTAT CCCACAAATT GATAAGTACT
             61 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG
            121 GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC CGACCCGTCA GTTGTCACTC
            181 CTTTCTCCAG AGATGACAGA GGTCAAACAC CCCTCCATGT GGCTGCCCTC TGTGGTCAGG
            241 CATCCCTCAT TGACTTCCTG GTCTCCAAAG GTGCCGTAGT GAATGCCACA GACTATCATG
            301 GGTCCACCCC CTTACATCTG GCATGTCAGA AAGGCTTCCA AAGTGTGACG CTGCTGCTGC
            361 TGCACTACAA GGCCAGCACT GAGGTTCAGG ACAACAACGG CAACACCCCG CTGCACCTGG
            421 CCTGCACCTA CGGGCAGGAG GATTGCGTGA AGGCCCTGGT CTACTATGAT GTCCAGGCCT
            481 GCAGATTGGA TATTGGTAAT GAGAAAGGAG ACACAGCCCT GCACATTGCT GCACGCTGGG
            541 GTTACGAGGG CATCATAGAG ACACTGCTGC AGAATGGAGC CCCCACAGCA GTCCAGAACA
            601 GACTGAAGGA AACACCACTC AAGTGTGCTC TGAACTCGAA GATTCTGTCT ATCATGGAAG
            661 CTCATCACCT ATCCTCTGAC AGGAGGCCAA GGCCTTCTGA GGTCCCTGCA CAGTCCCCTA
            721 CACGCTCTGT GGATTCCATC AGCCAGGGCT CCTCCACCTC CAGCTTTTCT TCCATATCGG
            781 TGAGCTTCAG GCAAGAAGAG GTCNAAAAGG ACTACAGGGN NGTAGAAAAG CTTCTAANGG
            841 CAGTTGCTGA TGGCGATCTA NAANTGGTAC GATANCTTTT GNAGTGGANA GANGATGACC
            901 TAGANNATGT GGAANANGCC NTCNGCACAG NGGATCTCGA ATTCTGNCNN CCCTNATGCC
            961 NNNNTCCCAN GTGNGCTCCA GCTCNNANNN TGGNNAGNAN TTGAANTCAC TNNNGNANNN
          1021 NCNNNCNGCN CTNNNNNGAG NNNCNNNNNT NGGNNNNNNN NNN
          //
          Primer: EF1a-M_R3 (Pr1255)
          Region: EF-1 alpha,SV40 pro_ori
          Sequence file: RDB17371_A9Fad.seq check
          >D06377B6_A9Fa_1_EF1a-M_R3_H03_24_ABI24.ab1
              1 NNNNNNNNNG NNNTCNNNNN NTTAAATGGC TAGAGACTTA TCGAAAGCAG CGAGACAGGC
             61 GCGAAGGTGC CACCAGATTC GCACGCGGCG GCCCCAGCGC CCAGGCCAGG CCTCAACTCA
            121 AGCACGAGGC GAAGGGGCTC CTTAAGCGCA AGGCCTCGAA CTCTCCCACC CACTTCCAAC
            181 CCGAAGCTCG GGATCAAGAA TCACGTACTG CAGCCAGGTG GAAGTAATTC AAGGCACGCA
            241 AGGGCCATAA CCCGTAAAGA GGCCAGGCCC GCGGGAACCA CACACGGCAC TTACCTGTGT
            301 TCTGGCGGCA AACCCGTTGC GAAAAAGAAC GTTCACGGCG ACTACTGCAC TTATATACGG
            361 TTCTCCCCCA CCCTCGGGAA AAAGGCGGAG CCAGTACACG ACATCACTTT CCCAGTTTAC
            421 CCCGCGCCAC CTTCTCTAGG CACCGGTTCA ATTGCCGACC CCTCCCCCCA ACTTCTCGGG
            481 GACTGTGGGC GATGTGCGCT CTGCCCACTG ACGGGCACCG GAGCCAATTC CCACTCCTTT
            541 CAAGACCTAG AAGGTCCATT AGCTGCAAAG ATTCCTCTCT GTTTAAAACT TTATCCATCT
            601 TTGCAAAGCT TTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT
            661 AGCTCAGAGG CCGANGCGGC CTCGGCCTCT GCATAAATAA AAAAAANTAG TCAGCCATGG
            721 GGCGGANAAT GGGCGGAACT GGGCGGANTT AGGGGCGGGA TGGGCGGANT TAGGGGCGGN
            781 NNTATGGTTG CTGACTAATT GANANGCATG CTTTGCNTAC TTCTGNCTGC TGGGGANGGA
            841 ANTAAGCTTG GCGTAANCAT GGNCATANNT GNTTNCTGTG TGAAATTGNN ATCCNNNNNN
          901 NNNTNCNNNN NNNNTACNAN NNNGNNNNNT NAAGNGNNAN NNNNNGGNNN NNNNNNN
          //
          Primer: EF1a-M_F3 (Pr1242)
          Region: EF-1 alpha
          Sequence file: RDB17371_A9Fae.seq check
          >D06377B6_A9Fa_1_EF1a-M_F3_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCT TGANTTGANG CCTGGCCTGG GCGCTGGGGC
             61 CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG CTGCTTTCGA TAAGTCTCTA
            121 GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA TAGTCTTGTA
            181 AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG GGGCCGCGGG CGGCGACGGG
            241 GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC TGCGAGCGCG GCCACCGAGA
            301 ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG TGCCTGGCCT CGCGCCGCCG
            361 TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG CACCAGTTGC GTGAGCGGAA
            421 AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT GGAGGACGCG GCGCTCGGGA
            481 GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT TTCCGTCCTC AGCCGTCGCT
            541 TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC TCGATTAGTT CTCGAGCTTT
            601 TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG CGATGGAGTT TCCCCACACT
            661 GAGTGGGTGG AGACTGNAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT
            721 TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCNNNCCT CNNACAGTGG TTCAAAGTTT
            781 TTTTCTTNNN NTTCNNGTGT CGTGAGGAAT TCTCTANAGC NGNNCGCNGG ANTTCNATTN
            841 CNCNATGGCT ANCATGACTG GTGGANNGCA AATGGGTCGC GNATNTATGT CCCCTATNNT
            901 NNNTTATTNG NAAAATNANN NNNNNNGTGN NNNCCNCNCN NNTTCTTTNN NNATATNNNN
            961 GNANNAAANT ANNNANANNA TTTNNTATNA NCGCNANNNA NNNNGANNAN TGNNNNANNN
          1021 AAAANNNNNN AANNNNNNNN NN
          //
          Primer: GST-N_rev (Pr0448)
          Region: GST 5',T7 tag,EF-1 alpha pro
          Sequence file: RDB17371_A9Faf.seq check
          >D06377B6_A9Fa_1_GST-N_rev_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCG AGTGGGTTGC ACAAGGCCCT TAATTTTCNN
             61 ATAACCTAGT ATAGGGGACA TAGATCCGCG ACCCATTTGC TGTCCACCAG TCATGCTAGC
            121 CATGGTGGAA TCGAATTCCC GCGGCCGCTC TAGAGAATTC CTCACGACAC CTGAAATGGA
            181 AGAAAAAAAC TTTGAACCAC TGTCTGAGGC TTGAGAATGA ACCAAGATCC AAACTCAAAA
            241 AGGGCAAATT CCAAGGAGAA TTACATCAAG TGCCAAGCTG GCCTAACTTC AGTCTCCACC
            301 CACTCAGTGT GGGGAAACTC CATCGCATAA AACCCCTCCC CCCAACCTAA AGACGACGTA
            361 CTCCAAAAGC TCGAGAACTA ATCGAGGTGC CTGGACGGCG CCCGGTACTC CGTGGAGTCA
            421 CATGAAGCGA CGGCTGAGGA CGGAAAGGCC CTTTTCCTTT GTGTGGGTGA CTCACCCGCC
            481 CGCTCTCCCG AGCGCCGCGT CCTCCATTTT GAGCTCCCTG CAGCAGGGCC GGGAAGCGGC
            541 CATCTTTCCG CTCACGCAAC TGGTGCCGAC CGGGCCAGCC TTGCCGCCCA GGGCGGGGCG
            601 ATACACGGCG GCGCGAGGCC AGGCACCAGA GCAGGCCGGC CAGCTTGAGA CTACCCCCGT
            661 CCGATTCTCG GTGGCCGCGC TCGCAGGCCC CGCCTCGCCG AACATGTGCG CTGGGACGCA
            721 CGGGCCCCGT CGCCGCCCGC GGCCCCNAAA ACCGAAATAC CAGTGTGCAG ATCTTGGCCC
            781 GCATTTACNA GANTATCTTG CCAGAAAAAA AGCGTCGCAG CANGTCATCN AAAATTTTAA
            841 ATGGNTAGAG NNTTATCGAA AGCAGCGAGA CAGGCGCGAA GGTGCCACCA GATTCNCACG
            901 NNGNCGGNCC CAGCGCCCAN GNCNNGNNTC NNCTCNNGCA CGAGGCGAAN GGGNNCCTTA
            961 ANCNCNNNNN NTCNAACTCT NCCACCCNNT TNCNNCCNNA ANCTCNGGNN NNNANNNNNN
          1021 NNNN
          //
          Primer: RDB17371_F1 (Pr1293)
          Region: insert 3',hG-CSF3' UTR+pA
          Sequence file: RDB17371_A9Fag.seq check
          >D06377B6_A9Fa_1_RDB17371_F1_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NCTAGNNGAN GTGGAAGANG CCNTCAGCAC AGTGGATCTC
             61 GAATTCTGTC ACCCCTTATG CCAGTGTCCC AAGTGTGCTC CAGCTCAGAA ACTGGCCAGG
            121 ATTTGAAATC ACTAGTGAAT TCGCGGCCGC TCTAGAGTGA GGGTCCCCAC CTGGGACCCT
            181 TGAGAGTATC AGGTCTCCCA CGTGGGAGAC AAGAAATCCC TGTTTAATAT TTAAACAGCA
            241 GTGTTCCCCA TCTGGGTCCT TGCACCCCTC ACTCTGGCCT CAGCCGACTG CACAGCGGCC
            301 CCTGCATCCC CTTGGCTGTG AGGCCCCTGG ACAAGCAGAG GTGGCCAGAG CTGGGAGGCA
            361 TGGCCCTGGG GTCCCACGAA TTTGCTGGGG AATCTCGTTT TTCTTCTTAA GACTTTTGGG
            421 ACATGGTTTG ACTCCCGAAC ATCACCGACG TGTCTCCTGT TTTTCTGGGT GGCCTCGGGA
            481 CACCTGCCCT GCCCCCACGA GGGTCAGGAC TGTGACTCTT TTTAGGGCCA GGCAGGTGCC
            541 TGGACATTTG CCTTGCTGGA CGGGGACTGG GGATGTGGGA GGGAGCAGAC AGGAGGAATC
            601 ATGTCAGGCC TGTGTGTGAA AGGAAGCTCC ACTGTCACCC TCCACCTCTT CACCCCCCAC
            661 TCACCAGTGT CCCCTCCACT GTCACATTGT AACTGAACTT CAGGATAATA AAGTGTTTGC
            721 CTCNNAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAN NAANNNNNNN
          781 AAAAAAAAAA NNNANAAAAA ANNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Marubashi, S., RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes. J. Biol. Chem. 291 (3): 1427-1440 (2016). PMID 26620560.
          reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl