Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDEST131NGFP_BAZ1A

Expression vector of human BAZ1A tagged with GFP at N-terminus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ohta S. et al. Mol. Cell Proteomics. 18(2), 169-181, 2019). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ohta S. et al. Mol. Cell Proteomics. 18(2), 169-181, 2019)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17333_A9Ehp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F () Region: EGFP 3', attB1, insert 5' Sequence file: RDB17333_A9Eha.seq
>D06261A1_A9Eh_1_mVenus-M_F_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CNTCNNGGNG ANNTTCANGA NCCGCCACNA NCATCGAGGN    61 CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT   121 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA   181 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT   241 GGACGAGCTG TACAAGGGTA CCACAAGTTT GTACAAAAAA GCAGGCTTCG AAGGAGATAG   301 AACCATGCCG CTGCTACACC GAAAGCCGTT TGTGAGACAG AAGCCGCCCG CGGACCTGCG   361 GCCCGACGAG GAAGTTTTCT ACTGTAAAGT CACCAACGAG ATCTTCCGCC ACTACGATGA   421 CTTTTTTGAA CGAACCATTC TGTGCAACAG CCTTGTGTGG AGTTGTGCTG TGACGGGTAG   481 ACCTGGACTG ACGTATCAGG AAGCACTTGA GTCAGAAAAA AAAGCAAGAC AGAATCTTCA   541 GAGTTTTCCA GAACCACTAA TTATTCCAGT TTTATACTTG ACCAGCCTTA CCCATCGTTC   601 GCGCTTACAT GAAATTTGTG ATGATATCTT TGCATATGTC AAGGATCGAT ATTTTGTCGA   661 AGAAACTGTG GAAGTCATTA GGAACAATGG TGCAAGGTTG CAGTGTANGA TTTTGGAAGT   721 CCTCCCTCCA TCACATCAAA ATGGTTTTGC TAATGGACAT GTNNACAGTG TGGATGGAGA   781 AACTATTATC ATCAGTGATA GTGATGATTC AGAAACACAN AGCTGTTCTT TTCAAANTGG   841 GAAGAAAAAA GATGCNATTG ATCCCTTACT ATTCANGTAT AAAGTGNNNC CCNCTAAAAA   901 ANAATTACAT GANTCTGCTA TTGTNNAAGC NACACAAATC ANCNGGANAA AACACCTATT   961 TTNNNGNGNT AAACNNAAAG CTTTTTTNNG ANNCAACNNN NNNNANNNNN NNGNNNNGNN 1021 NNNNNNNN //
Primer: BGH_rev2 () Region: BGH poly(A) signal, attB2, insert 3' Sequence file: RDB17333_A9Ehb.seq
>D06261A1_A9Eh_1_BGH_rev2_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNGGGG GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT    61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTAC TTTAGACCAC TTTGTACAAG   121 AAAGCTGGGT CATAGATTCG TGACTTTTTC GCAGCCGGTG GTGTGCTAAC TTGGTCCACA   181 TTACTGGGTG TGACGTGGAG TCCAAGCTTT TGAGCCTGAA TATGAAAAAA TGCTTGAAGC   241 CTAGTTCCAG CTTTTGCTTC ACTTGTGTTA CGAGGGTTGT ATTCAAAGCA GTTCGAAAAC   301 ATTAACTCAA TGTCATCAAT AAACTCAGAT GCTAATTTAT ATTCACACTT ATTCACTTTT   361 TCACGAATTA TATTTAAGGC AATGGGCTTT TTGATGATGT CATAGTAGTC TGGGACCTGG   421 ATTTTAGAAA CAAGTTTCAA AAAAGGCCAG CTGTCATCAT GTCGTACCAA TTCTACAACA   481 AGTTGTTCAA AAGCAGACAA TTCATGAACT CCTCCCTGTC GGCCAGAACT CCTTCGACCC   541 AGTGTCACAG GCGATGGCTC TGGAGATTGT CTTTTTCTGC ATCTTCTTTT GGATTCACTC   601 TCTTGGAGAG AAAGTTTTGA AGCAATATTT ACAGATCTTG ACTGTTCACT TGACTTTGTG   661 GCAATGACTC TGAAGTTAGG GAAGTTGGGA CTATTTTCTG GTGTATTATT AGCACTTTTC   721 CTGCCTCTGC GTTTTCTACG ANGANTAAGC AATTCCACAN ATACATCTGC TTGCAGTGNG   781 CCATGACTGT GGCGAGTANA ACGACTGGCA ATTCTTAAAG ATTTTGTTTC TGTANGANGA   841 NCGGANTTTA TCTTTCTTAN GCTTCTANNA GTTTTAGAAG AAACAGTTGT TTTGGGTGTG   901 CTNTGCTGAN TCCTNGNANN GNTATCTTCC NNNTTCTNGT TGNTGGNNNN NANTTGANAA 961 AGNNNNNCTN NNTTTNCNNN NTNNNTTNNN NNNGNNNNNN NNNN //
Primer: CMV-Forward () Region: CMV promoter, EGFP 5' Sequence file: RDB17333_A9Ehc.seq
>D06261A1_A9Eh_1_CMV_Forward_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGCNNTGGGC GGTANGCGTG TACGGTGGGN NGTCTATATA    61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG   121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACG ATGGTGAGCA   181 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA   241 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA   301 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA   361 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT   421 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG   481 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA   541 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT   601 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG   661 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC   721 AGCAGAACAC CCCCATCGGC GACNGNCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA   781 CCCAGTCCGC CCTGANCAAA GANCCCAACG ANAANCGCGA TCACATGGTC CTGCTGGANT   841 TCGNGANCGC CGCCNGGGAT CACTCTCGGC ANNGANGANC TGTNNNNNGG GTANCANNAG   901 TTTGTACNAA AAAANCNNNN TNCNAANGAN NNAGNANNNT GNNNNNNCNN CNCCNANNNN 961 NNNTTGNGAN ANANNNNNCN NNNNNNNGNN NNNNNNNN //
Primer: SV40polyA-R2 () Region: SV40 poly(A) signal, NeoR/KanR Sequence file: RDB17333_A9Ehd.seq
>D06261A1_A9Eh_1_SV40polyA-R2_D01_10_ABI24.ab1     1 NNNNNNNNNN NNCNNNNNNN NNGTTGGGGT GGGCGAANAA NTCCNGCATG AGNTCCCCGC    61 GCTGGAGGAN CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA   121 AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT   181 CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG   241 CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG   301 CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGGTCCG CCACACCCAG   361 CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA   421 GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCGCGCCT TGAGCCTGGC   481 GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG   541 ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG   601 GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT   661 CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG   721 CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT   781 GGCCAGCCAC GATAGCCGCG CTGCCTCGTC CTGCAGTTCA TTCANGGCAC NGGACAGGTC   841 GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGANAGC CGGAACACGG CGGCATCAGA   901 NCAGCCNANT GTCNGTTGNG CCCANNCATN NCNNANNNGC CNNNCCNCCC NAGCNGNCGG 961 NNNNNNNGCN NNNNNTCCNN NNNNNTCNNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl