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CSU6EG_sh7T-hFoxP3

Short hairpin RNA (shRNA) lentivirus expression vector of human FoxP3.

Catalog number RDB17321
Resource name CSU6EG_sh7T-hFoxP3
Clone info. Short hairpin RNA (shRNA) lentivirus expression vector of human forkhead box P3 (FoxP3) shRNA, U6 promoter, GFP. The target sequences is 5'-GCATGTTTGCCTTCTTCAGAA-3' with a haripin 5'-AGAGCTTG-3'.
Comment Expression was confirmed by the deposotor with the observation of GFP expression.
Vector backbone CSU6EG (plasmid)
Size of vector backbone 10 kb
Selectable markers Amp^r.
Gene/insert name human FOXP3 cDNA
Depositor|Developer Yoshitomi, Hiroyuki |
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External Database
human FOXP3

          Reference sequence
            
           
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kobayashi S. et al. Eur. J. Immunol. 46(2), 360-371, 2016). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
          Additional terms and conditions:
          The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
          Ordering Please visit Ordering instruction.[link] 
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          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kobayashi S. et al. Eur. J. Immunol. 46(2), 360-371, 2016)。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
          付加的提供条件:
          レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB17321 CSU6EG_sh7T-hFoxP3 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
          Ordering instruction of plasmids [in Japanese] [in English]

          References and tips

          Original reference

          original Kobayashi, S., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Kobayashi, S., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894.

          2023.04.06

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