Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-Rab12

Expression vector of mouse Rab12 fused with EGFP.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17259_A9Elp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: VN_F1 (Pr0992) Region: EGFP 3',insert 5' Sequence file: RDB17259_A9Ela.seq
>D06280E5_A9El_2_VN_F1_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGCN NCNNNGTCTA TATNNTGGCC GANNAGCAGA AGAACGGCAT    61 CAAGGTGAAC NNNNNNNTCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA   121 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT   181 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT   241 GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGACT   301 CAGATCTATG GATCCCAGCG CCGCGCTGCA CCGACGGCCC GCGGGCGGCA GCCTGGGCGC   361 GGTCTCCCCG GCGCTGTCGG GCGGCCAGGC CCGCCGGAGG AAGCAGCCCC CCAGGCCGGC   421 CGACTTCAAG CTGCAGGTCA TCATCATCGG CTCCCGCGGC GTGGGCAAGA CCAGCCTGAT   481 GGAGCGCTTC ACCGACGACA CGTTCTGCGA GGCCTGCAAG TCCACCGTGG GTGTTGACTT   541 TAAAATCAAG ACTGTAGAAC TAAGAGGGAA GAAAATTAGA TTACAGATCT GGGACACAGC   601 AGGGCAGGAG CGATTTAACA GCATTACCTC AGCCTATTAC AGAAGTGCCA AGGGGATCAT   661 ACTCGTATAT GACATCACCA AGAAGGAGAC GTTCGATGAC TTGCCNAAGT GGATGAAGAT   721 GANTGACAAG TATGCTTCAG AAGACGCCGA GCTCCTCCTG GTTGGAAANN NNNNGGACTG   781 TGAGACAGAC AGAGAAATCT CANGGCAGCA AGGANAAAAG TTNGCACAGC ANANAACCGG   841 GATGCGCTTC TGCNAANCCN GTGCCAANGN NANTTTCNAN GTGGNNNANN TCTTTCNGAA   901 ACTCGNNGAN NNNATTCTGA AAAANANGNN NNTNGNNNNN NNNGNANNNN NNNNNTCNNN 961 NANCNTNCNN TTCNNNNNNA NCNNNNNNNN NN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB17259_A9Elb.seq
>D06280E5_A9El_2_SV40polyA-R2_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNANNNNNA GTTAANNANA ACNATTGCAT TCNTTTTATG TTTCNGGTTC    61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG   121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA   181 TTCACTAGTG ATTCTCCAAA GTATCAAATC AACAGCATCG GACGTGTGGT CTGGGAGGAG   241 GCAGCTCTGG TGGGATCTCA GGCTCTGGTT GTAGAGAAAG GATGCTATTG GATAACTCGT   301 TTCTCAAAAC ATCAAGAGGC ATCTTTTTCA GAATATCATC AACGAGTTTC AGAAAGATCT   361 CGTCCACATT GAAATTGTCC TTGGCACTGG CTTCGCAGAA GCGCATCCCG GTTATCTGCT   421 GTGCAAACTT TTCTCCTTGC TGCCTTGAGA TTTCTCTGTC TGTCTCACAG TCCAGCTTAT   481 TTCCAACCAG GAGGAGCTCG GCGTCTTCTG AAGCATACTT GTCAATCATC TTCATCCACT   541 TTGGCAAGTC ATCGAACGTC TCCTTCTTGG TGATGTCATA TACGAGTATG ATCCCCTTGG   601 CACTTCTGTA ATAGGCTGAG GTAATGCTGT TAAATCGCTC CTGCCCTGCT GTGTCCCAGA   661 TCTGTAATCT AATTTTCTTC CCTCTTAGTT CTACAGTCTT GATTTTAAAG TCAACACCCA   721 CGGTGGACTT GCAGGCCTCG CAGAACGTGT CGTCNGTGAA GCGCTCCATC ANGCTGGTCT   781 TGCCCACGCC GCGGGANCCG ATGANGANGA CCTGCAGCTT GAAGTCGGCC GGCCTGGGGG   841 GCTGCTTNCT CCGGCGGGCC TGGCCGCCCG ANAGCGCCNG GGANANCGCG CCCNGNCTGN   901 CGCCCGCGGG CCGTCGGTGC ANCNCNNCGC TNGGNTNCAT ANATCTGANT NNGGANNNGT   961 ACAGCTCGTC CNTGCCGANA NTGNTNCNNG NNNNNGNNNN NNANNNNNAN CNNNANNNTG 1021 NGATNNNNNN NNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP 5' Sequence file: RDB17259_A9Elc.seq
>D06280E5_A9El_2_CMV-Forward_G03_21_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTGN NGCNNNTGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT    61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG   121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC   181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG   241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG   301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC   361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG   421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC   481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG   541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC   601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC   661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG   721 AGCACCCAGT CCGCCCTGAG CAAAGANCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG   781 GAGTTCGTGA NCGCCGCCGG GATCACTCTC GGCATGGNNG ANCTGTACNA GTNCNGANTC   841 AGATCTATGG ATNCCAGCGC CGCGCTGCAC CGACNGNCCG CGGGCGGNAG NCTGGGCGCG   901 GNCNNCCCGG NGCTNNNGGG CNGNCAGGCC CNCCNGNANG AANCANCCCN NNNNNNCNNN   961 NCGANTTNNN GCTNNNNNNC NTNNNCATNN NNTNCNNCNN NNNGGNNNNN ANNNNNCNNN 1021 NNNGNNNCNN NNNNNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB17259_A9Eld.seq
>D06280E5_A9El_2_SV40pro_F_V2_H03_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGCTTTNNN NNNNNNCGAT CNAGAGACAG GATGAGGANC    61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT   601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA   721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA   781 CCGCTTCCTC GTGCTTTACN GTATCGCCGC TCCNGATTCG CAGCGCATCG CCTTCTATCG   841 CCTTCTTGAC GAGTTCTTCT GAGCGGGANT CTNGGGTNCG AAATGACCGA NCAAGCGACG   901 CCCAACCTGC CNTCACGAGA TTTCNATTCC NNCGCCGCCT TCTATGAAAN GNNGGGCTTC   961 NGANNCNTTT TNCNGGGANG NNNGNTGGNN GATNCNTNNN NCNNGGGGNN NNCANGNNNG 1021 NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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