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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab6D

Expression vector of human Rab6D (Rab41 in NCBI) fused with EGFP.

Catalog number RDB17252
Resource name pEGFP-C1-Rab6D
Clone info. Expression vector of human Rab6D [Rab41 in NCBI] fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human RAB41 cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
human RAB41
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aizawa, M. & Fukuda, M. (2015) J. Biol. Chem. 290, 22250-22261)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aizawa, M. & Fukuda, M. (2015) J. Biol. Chem. 290, 22250-22261)。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB17252 pEGFP-C1-Rab6D DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17252_A9Elp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 ()
          Sequence file: RDB17252_A9Ela.seq check
          >D06280D7_A9El_2_EGFP-C2_A09_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGNN CNCNTGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
             61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGTCTGCC TTTGGTCACG
            121 ACGAGGCCTG GATGGAGGCC GGAGGCTTTG GTCTGGAGGC TGCCGAAAGA ACGGAATACC
            181 AGTCTCTGTG CAAATCTAAA CTCTTATTCC TGGGAGAGCA GAGCGTAGGG AAGACATCCA
            241 TCATCAGCCG CTTCATGTAC AACAGCTTCG GCTGCGCCTG CCAGGCAACT GTTGGAATTG
            301 ACTTCTTGTC TAAGACCATG TACTTGGAGG ACCAAATAGT TCAGCTGCAG CTATGGGACA
            361 CAGCTGGCCA GGAGCGCTTT CACAGCCTAA TTCCTAGCTA CATTCGTGAT TCAACTATTG
            421 CAGTGGTTGT CTATGACATT ACAAACATCA ATTCTTTTAA GGAGACAGAT AAGTGGGTAG
            481 AACACGTGCG AGCAGAAAGA GGTGACGATG TTGTCATCAT GTTGTTGGGT AACAAGATTG
            541 ATTTGGATAA CAAAAGACAA GTCACTGCAG AACAGGGTGA AGAAAAATCC AGAAACCTCA
            601 ATGTGATGTT TATTGAGACC AGTGCCAAAA CCGGTTACAA CGTGAAAAAG CTGTTCCGGC
            661 GTGTGGCTTC TGCCCTTCTT TCCACAAGGA CTTCACCTCC ACCAAAAGAG GGGACGGTTG
            721 AAATCGAACT GGAATCCTTC GAGGAGTCAN GCAACAGAAG CTATTGTTGA AATCNCTAGT
            781 GAATTCNCGG NCGCCTGCNN GNCGACNGTA NCGCGGGCCC NGGATNNNNN NGATCNAGAT
            841 AACTGATCAT AATCNGCNNN ACCACNTTTG TANAGGTTTN NNTNGCTTTA AAAAANNTCC
            901 CNCNCCTCCC CNNNNNNNTG AANNNTNAAA TGNNNGNNNN NNNNGNNGNN NNNNNNNTNN
          961 NNNNNNCNNN NNNNNNN
          //
          Primer: SV40polyA_R2 ()
          Sequence file: RDB17252_A9Elb.seq check
          >D06280D7_A9El_2_SV40polyA-R2_F07_16_ABI24.ab1
              1 NNNNNNNNNN NNNCNNNNNN NNNGTTNNCA ACNACAATTG CATTCATTTT ATGTTTCAGG
             61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
            121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACCTGC
            181 AGGCGGCCGC GAATTCACTA GTGATTTCAA CAATAGCTTC TGTTGCCTGA CTCCTCGAAG
            241 GATTCCAGTT CGATTTCAAC CGTCCCCTCT TTTGGTGGAG GTGAAGTCCT TGTGGAAAGA
            301 AGGGCAGAAG CCACACGCCG GAACAGCTTT TTCACGTTGT AACCGGTTTT GGCACTGGTC
            361 TCAATAAACA TCACATTGAG GTTTCTGGAT TTTTCTTCAC CCTGTTCTGC AGTGACTTGT
            421 CTTTTGTTAT CCAAATCAAT CTTGTTACCC AACAACATGA TGACAACATC GTCACCTCTT
            481 TCTGCTCGCA CGTGTTCTAC CCACTTATCT GTCTCCTTAA AAGAATTGAT GTTTGTAATG
            541 TCATAGACAA CCACTGCAAT AGTTGAATCA CGAATGTAGC TAGGAATTAG GCTGTGAAAG
            601 CGCTCCTGGC CAGCTGTGTC CCATAGCTGC AGCTGAACTA TTTGGTCCTC CAAGTACATG
            661 GTCTTAGACA AGAAGTCAAT TCCAACAGTT GCCTGGCAGG CGCAGCCGAA GCTGTTGTAC
            721 ATGAAGCGGC TGATGATGGA TGTCTTCCCT ACGCTCTGCT CTCCCAGGAA TAAGAGTTTA
            781 GATTTGCACA GAGACTGGTA TTCCGTTCTT TNGGCAGCCT CCAGANCAAA GCCTCCNGCC
            841 TCNATCCAGG CCTCGTCGTG ACCNAAGGCA GANATGGATC TGANNNCGGA NTTGTACAGC
            901 TCGTCNNTGC CNAGAGTGAT CCCGGNNGCG GNCACGAACT NCAGCNNNAN NNTGTGATCN
            961 CNCTTCNCGT TGGGGNNNTT GCTCNNGNNN NANNGGGNNN TCNNNANTNN NNNNNNGGNN
          1021 NNNNCNNNGG NNNNNNNNNN
          //
          Primer: CMV_Forward ()
          Sequence file: RDB17252_A9Elc.seq check
          >D06280D7_A9El_2_CMV-Forward_C09_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCCNN NTTGNNNCNA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
             61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA
            121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
            181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
            241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
            301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
            361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
            421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
            481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
            541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
            601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
            661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
            721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC
            781 TGNNNNNNTT CGTGACCGCC GCCGGGNTCA CTCTCGGCNT GGACNANCTG TACAAGTCCG
            841 GACTCAGATC CATGTCTGCC TTTNGTCACN NCGANGCCTG NATGGANGNC GGAGGCTTTN
            901 GTCTGGANGC TGCCGAAANA NNNGNANTAC CNGTCTCTGN GNNAATCNNN NTCNNATTNC
            961 TGGGANANCN GANCNNNNNG GGAAGANNTC CATCNTCNNN CCNNNNTNNN NNNNNNANAN
          1021 NNTNNNNNNN NNCNNNNCNN NGNNANNNNN NGNNNNNNNN NTNNNNNNNN NN
          //
          Primer: SV40pro_F_V2 ()
          Sequence file: RDB17252_A9Eld.seq check
          >D06280D7_A9El_2_SV40polyA-R2_F07_16_ABI24.ab1
              1 NNNNNNNNNN NNNCNNNNNN NNNGTTNNCA ACNACAATTG CATTCATTTT ATGTTTCAGG
             61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
            121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACCTGC
            181 AGGCGGCCGC GAATTCACTA GTGATTTCAA CAATAGCTTC TGTTGCCTGA CTCCTCGAAG
            241 GATTCCAGTT CGATTTCAAC CGTCCCCTCT TTTGGTGGAG GTGAAGTCCT TGTGGAAAGA
            301 AGGGCAGAAG CCACACGCCG GAACAGCTTT TTCACGTTGT AACCGGTTTT GGCACTGGTC
            361 TCAATAAACA TCACATTGAG GTTTCTGGAT TTTTCTTCAC CCTGTTCTGC AGTGACTTGT
            421 CTTTTGTTAT CCAAATCAAT CTTGTTACCC AACAACATGA TGACAACATC GTCACCTCTT
            481 TCTGCTCGCA CGTGTTCTAC CCACTTATCT GTCTCCTTAA AAGAATTGAT GTTTGTAATG
            541 TCATAGACAA CCACTGCAAT AGTTGAATCA CGAATGTAGC TAGGAATTAG GCTGTGAAAG
            601 CGCTCCTGGC CAGCTGTGTC CCATAGCTGC AGCTGAACTA TTTGGTCCTC CAAGTACATG
            661 GTCTTAGACA AGAAGTCAAT TCCAACAGTT GCCTGGCAGG CGCAGCCGAA GCTGTTGTAC
            721 ATGAAGCGGC TGATGATGGA TGTCTTCCCT ACGCTCTGCT CTCCCAGGAA TAAGAGTTTA
            781 GATTTGCACA GAGACTGGTA TTCCGTTCTT TNGGCAGCCT CCAGANCAAA GCCTCCNGCC
            841 TCNATCCAGG CCTCGTCGTG ACCNAAGGCA GANATGGATC TGANNNCGGA NTTGTACAGC
            901 TCGTCNNTGC CNAGAGTGAT CCCGGNNGCG GNCACGAACT NCAGCNNNAN NNTGTGATCN
            961 CNCTTCNCGT TGGGGNNNTT GCTCNNGNNN NANNGGGNNN TCNNNANTNN NNNNNNGGNN
          1021 NNNNCNNNGG NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634.

          2023.04.06

          GNP_filter3_RDBDEP_html_230405.pl