Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-Rab38-T23N

Expression vector of mouse Rab38 constitutive negative mutant, T23N, fused with EGFP at N-terminus.

Sequence (full)	RDB16899zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16899_A9A8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP 3',insert (T23N) Sequence file: RDB16899_A9A8a.seq
>D06060I1_A9A8_1_EGFP-C2_C06_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNCGNN CNCNTGGTCC TGCTGGNNTT CGTGNNCGCC GCCGGGATCA    61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGCAGACA CCTCACAAGG   121 AGCACCTGTA CAAGCTGCTG GTGATCGGCG ACCTGGGTGT GGGCAAGAAC AGCATTATCA   181 AGCGCTATGT GCACCAAAAC TTCTCCTCGC ACTACCGGGC CACCATTGGT GTGGACTTCG   241 CGCTGAAGGT GCTCCACTGG GACCCAGAGA CGGTGGTGCG CTTGCAGCTC TGGGACATTG   301 CTGGTCAAGA AAGATTTGGA AACATGACAA GAGTTTATTA CCGGGAAGCT ATGGGGGCAT   361 TTATTGTTTT TGATGTCACC AGACCAGCCA CATTTGAAGC CGTGGCAAAG TGGAAAAATG   421 ATTTGGACTC AAAGTTAACG CTCCCTAATG GTAAGCCAGT GTCAGTGGTT CTGTTGGCCA   481 ACAAATGTGA CCAAGGGAAG GATGTGCTTA TGAACAATGG ACTCAAGATG GACCAGTTCT   541 GCAAGGAGCA TGGCTTCGTA GGATGGTTTG AAACATCAGC CAAGGAAAAC ATAAACATTG   601 ATGAAGCCTC AAGATGCCTG GTCAAGCACA TACTTGCAAA TGAGTGTGAC CTCCTAGAGT   661 CTATAGAACC GGACATTGTG AAGCCCCATC TCACATCGCC CAAGGTTGTC AGCTGCTCTG   721 GCTGTGCCAA ATCCTAGAAT CACTAGTGAA TTCTGCAGTC GACGGTACCG CGGGCCCGGG   781 ATCCACCGGA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT   841 TGCTTTAAAA AACCTCCCAC ACCTCCCCCN GANNNTGAAA CATAAAATGA ATGNAATTGN   901 TGTTGNNNAC TNGTTTATTG CAGCTTATAA TGGNTNNNAA TAAAGCAATA NCATCNCAAA 961 NTTTCNNNAA TAAAGCANTT TTTTTCNNTG NNNNCNANNN NNNNNTNNNN NNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert (T23N) Sequence file: RDB16899_A9A8b.seq
>D06060I1_A9A8_1_SV40polyA_R2_D06_12_ABI24.ab1     1 NNNNNNNNNN NNNANNNNNN NTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA    61 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA   121 TTATGATCAG TTATCTAGAT CCGGTGGATC CCGGGCCCGC GGTACCGTCG ACTGCAGAAT   181 TCACTAGTGA TTCTAGGATT TGGCACAGCC AGAGCAGCTG ACAACCTTGG GCGATGTGAG   241 ATGGGGCTTC ACAATGTCCG GTTCTATAGA CTCTAGGAGG TCACACTCAT TTGCAAGTAT   301 GTGCTTGACC AGGCATCTTG AGGCTTCATC AATGTTTATG TTTTCCTTGG CTGATGTTTC   361 AAACCATCCT ACGAAGCCAT GCTCCTTGCA GAACTGGTCC ATCTTGAGTC CATTGTTCAT   421 AAGCACATCC TTCCCTTGGT CACATTTGTT GGCCAACAGA ACCACTGACA CTGGCTTACC   481 ATTAGGGAGC GTTAACTTTG AGTCCAAATC ATTTTTCCAC TTTGCCACGG CTTCAAATGT   541 GGCTGGTCTG GTGACATCAA AAACAATAAA TGCCCCCATA GCTTCCCGGT AATAAACTCT   601 TGTCATGTTT CCAAATCTTT CTTGACCAGC AATGTCCCAG AGCTGCAAGC GCACCACCGT   661 CTCTGGGTCC CAGTGGAGCA CCTTCAGCGC GAAGTCCACA CCAATGGTGG CCCGGTAGTG   721 CGAGGAGAAG TTTTGGTGCA CATAGCGCTT GATAATGCTG TTCTTGCCCA CACCCAGGTC   781 GCCGATCACC AGCAGCTTGT ACAGGTGCTC CTTGTGANGT GTCTGCATGG ATCTGANTCC   841 GGANTTGTAC AGCTCGTCCA TGCCGANAGT GATNCNGGCG GCGGTCACGA ACTNCANCAN   901 GANCNTGTGA TCGCNCTTCN CNNTNGGGNC TTNNCTCNNN NCGGANNGGG NGNTCNNNNN 961 NGGNNGNCGG GNANCANCNC GGGGNCNNNN NNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP Sequence file: RDB16899_A9A8c.seq
>D06060I1_A9A8_1_CMV_Forward_C05_08_ABI24.ab1     1 NNNNNNNNNN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC   121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA   181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG   241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC   301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC   361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC   421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC   481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG   541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG   601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC   661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC   721 ACCCAGTCCG CCCTGANCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAN   781 TTCGTGANCG CCGCCGGGAT CACTCTCGGC ATGGNCGANC TGTACNAGTC CGGANTCAGA   841 TCCNTGCAGA CACCTCACAA GGANCACCTG TACAAGCTGC TGGNGNTCGG CGANNNNNNT   901 GNGGGCNAGA ANNGCATTAT CNANCGCTNT GNGCNNNNAA NNNTCNNNNN GCACTACCNG 961 GNCNNCNNNN NNNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB16899_A9A8d.seq
>D06060I1_A9A8_1_SV40pro_F_V2_E06_15_ABI24.ab1     1 NNNNNNNNNN NGNNCNNNGC TTTTGCAAGN NCGATCAAGA GNNAGGNTGA GGNTCGTTTC    61 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT   121 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT   181 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC   241 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG   301 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC   361 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA   421 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC   481 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG   541 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG   601 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA   661 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCAGG   721 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT   781 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC   841 TTGACGANTT CTTCTGANCG GGANTCTNGG GTTCGAAATG ANCGACCAAG CGACGCCCNA   901 CCTGCCNTCA CGAGATTTCG ANNNCNCCGC CNNCTTCTAT GAAAGGNTGG NNTTCNNNNN 961 NNTTTTNNNG GNNNNCNNNN NGNNNGATNN NNCNNNNNNN GGNNNNNNNN NN //
Primer: mVenus-M_F (Pr0574) Region: EGFP 3',insert (T23N) Sequence file: RDB16899_A9A8e.seq
>16899_16899_B1JdF_mVenus-M_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNANNGTN NNNCTTCNNN NATCCGCCNC NNCATCNNNG    61 ACNGNNNCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG   121 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG   181 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA   241 TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGCAGAC ACCTCACAAG GAGCACCTGT   301 ACAAGCTGCT GGTGATCGGC GACCTGGGTG TGGGCAAGAA CAGCATTATC AAGCGCTATG   361 TGCACCAAAA CTTCTCCTCG CACTACCGGG CCACCATTGG TGTGGACTTC GCGCTGAAGG   421 TGCTCCACTG GGACCCAGAG ACGGTGGTGC GCTTGCAGCT CTGGGACATT GCTGGTCAAG   481 AAAGATTTGG AAACATGACA AGAGTTTATT ACCGGGAAGC TATGGGGGCA TTTATTGTTT   541 TTGATGTCAC CAGACCAGCC ACATTTGAAG CCGTGGCAAA GTGGAAAAAT GATTTGGACT   601 CNAAGTTAAC GCTCCCTAAT GGTAAGCCNG TGTCAGTGGT TCNGTTGNNC NACNAATGNG   661 ANCNNNGNNA NGNANGTGCT TNNGANCNNT GGNNTCANNN GGACNNNTTC TGNNNGNNNC 721 NNNNNNNCNN NNNNNNNTNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl