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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab32-T37N

Expression vector of mouse Rab32 constitutive negative mutant, T37N, fused with EGFP at N-terminus.

Catalog number RDB16890
Resource name pEGFP-C1-Rab32-T37N
Clone info. Expression vector of mouse Rab32 constitutive negative mutant, T37N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab32 cDNA
Reference of insert sequence AB232627 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab32

          Reference sequence
            
           
          Sequence (full) RDB16890zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。
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          RDB16890 pEGFP-C1-Rab32-T37N DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEGFP-C1-Rab32-T37N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16890).

          Reference section:

          Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          Featured content

          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16890_A9A8p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP 3',insert(T37N)
          Sequence file: RDB16890_A9A8a.seq check
          >D06060H1_A9A8_3_EGFP-C2_E12_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNCGNTC NCNTGGTCCT GCTGGNNTTC GTGACCGCCG CCGGGATCAC
             61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGGCGGGCG AGGGACTAGG
            121 GCAACAGGGC GCGTCGGCCA CCGCGGCGCC GGAGACCCGA GAGCACCTCT TTAAGGTGCT
            181 GGTCATCGGC GAGCTCGGCG TGGGTAAGAA CAGCATCATC AAGCGCTACG TGCACCAGCT
            241 CTTCTCCCAG CACTACCGCG CCACCATTGG CGTGGACTTC GCCCTCAAAG TTCTCAACTG
            301 GGACAGCAGG ACGCTCGTGC GCCTGCAGCT GTGGGATATC GCGGGACAGG AACGGTTTGG
            361 CAACATGACT CGAGTATACT ATAAGGAAGC TCTTGGAGCA TTTGTAGTCT TTGATATATC
            421 GAGAAGTTCT ACATTTGATG CAGTCCTAAA ATGGAAAAAC GATCTGGATA GTAAAGTTCA
            481 TCTTCCCAAC GGCAGCCCCA TTCCTGCTGT CCTCCTAGCT AACAAATGTG ACCAGAAGAA
            541 GGACAACAGC CAGAGTCCTT CCCAGATGGA CCAGTTTTGT AAAGACCATG GCTTCACTGG
            601 ATGGTTTGAA ACCTCTGCCA AGGATAATAT AAACATCGAC GAGGCCACCC GCTTCCTAGT
            661 GGAAAACATG CTTGCAAACC AGCAAAGTTT TCCTAGTGAA GAAATCGACC TGGACAGAAT
            721 TAAACTGGTA GAGGAGCCCC CCACAACAAA GCCCAGGTCC CAGTGCTGCT GANGTGTCTC
            781 TGTGAATCAC TAGTGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGANC CACCGGNTCT
            841 AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG NTTTACTTGC TTTAAAAAAN
            901 CTCCCACACC NCCCCNNGAA NCTGAAANNT AAAATGAANG NNNTTGNTGN TGNTNACNTG
            961 NTTANNGNAN CTTANNNNGN NNANNAATAA ANNNANANCA TNNNNAANTN NNNNNNNANN
          1021 NNTTTTTTNN NNNNNNNN
          //
          Primer: SV40polyA_R2 (Pr0623)
          Region: insert(T37N)
          Sequence file: RDB16890_A9A8b.seq check
          >D06060H1_A9A8_3_SV40polyA-R2_F12_18_ABI24.ab1
              1 NNNNNNNNNC NGNNNNNNNN NNGTTAACNN NNACAATTGC ATTCNTTTTA TGTTTCNGGT
             61 TCNGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
            121 TGATTATGAT CAGTTATCTA GATCCGGTGG ATCCCGGGCC CGCGGTACCG TCGACTGCAG
            181 AATTCACTAG TGATTCACAG AGACACATCA GCAGCACTGG GACCTGGGCT TTGTTGTGGG
            241 GGGCTCCTCT ACCAGTTTAA TTCTGTCCAG GTCGATTTCT TCACTAGGAA AACTTTGCTG
            301 GTTTGCAAGC ATGTTTTCCA CTAGGAAGCG GGTGGCCTCG TCGATGTTTA TATTATCCTT
            361 GGCAGAGGTT TCAAACCATC CAGTGAAGCC ATGGTCTTTA CAAAACTGGT CCATCTGGGA
            421 AGGACTCTGG CTGTTGTCCT TCTTCTGGTC ACATTTGTTA GCTAGGAGGA CAGCAGGAAT
            481 GGGGCTGCCG TTGGGAAGAT GAACTTTACT ATCCAGATCG TTTTTCCATT TTAGGACTGC
            541 ATCAAATGTA GAACTTCTCG ATATATCAAA GACTACAAAT GCTCCAAGAG CTTCCTTATA
            601 GTATACTCGA GTCATGTTGC CAAACCGTTC CTGTCCCGCG ATATCCCACA GCTGCAGGCG
            661 CACGAGCGTC CTGCTGTCCC AGTTGAGAAC TTTGAGGGCG AAGTCCACGC CAATGGTGGC
            721 GCGGTAGTGC TGGGAGAAGA GCTGGTGCAC GTAGCGCTTG ATGATGCTGT TCTTACCCAC
            781 GCCGAGCTCG CCGATGACCA GCACCTTAAA GANGTGCTCT CGGGTCTCCG GCGCCGCGGN
            841 GGCCGACGCG CCCTGTTGNC CTANTCCNTC GCCCGCCATG GATCTGANTN CNGANTTGTA
            901 CAGCTCGNNC ATGCCNAGAG TGATCCCNGN GGNNGNCACG AACTCCANCA NGANNNTGNG
            961 ATNNCNCTTC NCNNNGGGGN TCNTTGCNCN NNNNGNANTG GGNGNNCNNN TANNNGNNNG
          1021 NNNNNNNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,EGFP
          Sequence file: RDB16890_A9A8c.seq check
          >D06060H1_A9A8_3_CMV_Forward_G12_21_ABI24.ab1
              1 NNNNNNNNNN NNNNTTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
            121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
            181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
            241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
            301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
            361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
            421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
            481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
            541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
            601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
            661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGANC
            721 ACCCAGTCCG CCCTGANCAA AGANCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAN
            781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGNCNANC TGTACNAGTC CGGANTCNNA
            841 TNNNTGGCGG GCGANGGNNT AGGGCAACAG GGNGCGNCGG NCNNCGCGGC GCCNGANNNC
            901 NGANANCACN NCTTTNANNN GCTGNNCNTC GNNNANCNNN GNNNNNNNNN ANNAGCATCN
          961 TCNNGCGCNN CNNGCNNNNN CNNNNNNNCN NNNNNNNNN
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR_KanR
          Sequence file: RDB16890_A9A8d.seq check
          >D06060H1_A9A8_3_SV40pro_F_V2_A05_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCNNN GCTTTTNNNN NNNCGATCAA GANACAGGNT GAGGNTCGTT
             61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
            121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
            181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
            241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
            301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
            361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
            421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
            481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
            541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
            601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
            661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA
            721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGANCG
            781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
            841 TCTTGACNAG TTCTTCTGAN CGGGNNTCTG GGGNTCGAAA TGACCGACCN AGCGACGCCC
            901 AACCTGNNNN NNCGAGATTT CGANTCCACC GCCNCCNTCT ATGAAANGNT GGGCTTCNGA
            961 ATCGTTTTNN NGGNNGNCNG GNNNNNNNAN NNNNCNNNNN NGGNNNNNNN NGNNNGNNNN
          1021 NNNNNN
          //
          Primer: mVenus-M_F (Pr0574)
          Region: EGFP 3',insert 5'(T37N)
          Sequence file: RDB16890_A9A8e.seq check
          >16890_16890_B1JdF_mVenus-M_F_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNA NNNNNNNNNT NNAGATCCGC CACAACATCG AGGACGGCAG
             61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
            121 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
            181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
            241 GCTGTACAAG TCCGGACTCA GATCCATGGC GGGCGAGGGA CTAGGGCAAC AGGGCGCGTC
            301 GGCCACCGCG GCGCCGGAGA CCCGAGAGCA CCTCTTTAAG GTGCTGGTCA TCGGCGAGCT
            361 CGGCGTGGGT AAGAACAGCA TCATCAAGCG CTACGTGCAC CAGCTCTTCT CCCAGCACTA
            421 CCGCGCCACC ATTGGCGTGG ACTTCGCCCT CAAAGTTCTC AACTGGGACA GCAGGACGCT
            481 CGTGCGCCTG CAGCTGTGGG ATATCGCGGG ACAGGAACGG TTTGGCAACA TGACTCGAGT
            541 ATACTATAAG GAAGCTCTTG GAGCATTTGT AGTCTTTGAT ATATCGAGAA GTTCTACATT
            601 TGATGCAGTC CTAAAATGGA AAAACGATCT GGATAGTNAA GTTCATCTTC CCNACNGCAG
            661 CCCCNTTCCT GCNGNNCTCC TANCTNACAN NGTGANNNGN ANNNNNNNAN NNNNNNANNN
          721 NNNCNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
          reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]
          reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]

          2024.03.26

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