Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-Rab27A-Q78L

Expression vector of mouse Rab27A constitutive active mutant, Q78L, fused with EGFP at N-terminus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. and Kuroda, T.S. J. Biol. Chem. 277, 43096-43103, 2002)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. and Kuroda, T.S. J. Biol. Chem. 277, 43096-43103, 2002)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16889_A9A8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP 3', insert 5' (Q78L) Sequence file: RDB16889_A9A8a.seq
>D06060G9_A9A8_1_EGFP-C2_A12_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCGN TCNCNTGGTC CTGCTGGANT TCGTGACCGC CGCCGGGATC    61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGTCGGA TGGAGATTAC   121 GATTACCTCA TCAAGTTCTT GGCCTTGGGA GACTCTGGGG TAGGGAAGAC CAGTGTACTC   181 TACCAGTACA CTGATGGCAA GTTCAACTCC AAATTCATCA CCACAGTGGG CATTGATTTC   241 AGGGAAAAGA GAGTGGTGTA CAGAGCCAAT GGGCCAGATG GAGCTGTGGG CCGAGGCCAG   301 AGAATCCACC TGCAGTTATG GGACACGGCG GGGCTGGAGA GGTTTCGGAG CTTAACCACT   361 GCATTCTTCA GGGACGCTAT GGGTTTCCTG CTTCTGTTCG ACCTGACAAA TGAGCAAAGT   421 TTCCTCAATG TCCGAAACTG GATAAGCCAG CTACAGATGC ACGCGTACTG TGAAAACCCA   481 GATATAGTGC TGTGTGGAAA TAAGAGTGAC CTGGAAGACC AGAGGGCAGT GAAAGAGGAG   541 GAGGCCCGGG AACTTGCCGA GAAGTACGGA ATCCCCTATT TTGAAACCAG TGCTGCCAAC   601 GGGACAAACA TAAGCCACGC GATTGAGATG CTCCTGGANC TGATCATGAA GCGGATGGAG   661 CGGTGTGTGG ACAAGTCCTG GATTCCGGAN GGAGTGGTAC GGTCCAACGG CCNTACCTCT   721 GCGGANCAGC TAAGTGANGA GAANGAGAAN GGGTTGTGTG GCTGTTGAAA TCACTAGTGA   781 ANTCTGCAGT CGANGGTACC GCGGGCCCNG GNNNCNNCNG ATCTNGATAA CNGATCNTAA   841 TCAGCCNTAC CANATTTGTA NANGNTTTAC TNGCTTTNAA AANNNTCCNN CACNNCCCNN 901 NNNNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB16889_A9A8b.seq
>D06060G9_A9A8_1_SV40polyA-R2_B12_06_ABI24.ab1     1 NNNNNNNNCN NNNNNNNNNA GTTAACNANN ACNATTGCAT TCATTTTATG TTTCAGGTTC    61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG   121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA   181 TTCACTAGTG ATTTCAACAG CCACACAACC CCTTCTCCTT CTCCTCACTT AGCTGATCCG   241 CAGAGGTATG GCCGTTGGAC CGTACCACTC CCTCCGGAAT CCAGGACTTG TCCACACACC   301 GCTCCATCCG CTTCATGATC AGGTCCAGGA GCATCTCAAT CGCGTGGCTT ATGTTTGTCC   361 CGTTGGCAGC ACTGGTTTCA AAATAGGGGA TTCCGTACTT CTCGGCAAGT TCCCGGGCCT   421 CCTCCTCTTT CACTGCCCTC TGGTCTTCCA GGTCACTCTT ATTTCCACAC AGCACTATAT   481 CTGGGTTTTC ACAGTACGCG TGCATCTGTA GCTGGCTTAT CCAGTTTCGG ACATTGAGGA   541 AACTTTGCTC ATTTGTCAGG TCGAACAGAA GCAGGAAACC CATAGCGTCC CTGAAGAATG   601 CAGTGGTTAA GCTCCGAAAC CTCTCCAGCC CCGCCGTGTC CCATAACTGC AGGTGGATTC   661 TCTGGCCTCG GCCCACAGCT CCATCTGGCC CATTGGCTCT GTACACCACT CTCTTTTCCC   721 TGAAATCAAT GCCCACTGTG GTGATGAATT TGGAGTTGAA CTTGCCATCA GTGTACTGGT   781 AGAGTACACT GGTCTTCCCT ACCCCAGANT CTCCCAANGC CAAGAACTTG ATGANGTAAT   841 CGNAATCTCC ATCCGANATG GNTCTGANNN CGGANNTGNN CAGCTCGTCC NNGNCNANAN   901 TGANNCCGGN GGCGGNCNCG ANNNNCNNCN NNANCNTGTG ATCNCNCNNN NCNNNGGGNN 961 CNTTGNNNNN GNCNNNNNNN NNGNNCNNNT ANNNNNNNNN N //
Primer: CMV_Forward Sequence file: RDB16889_A9A8c.seq
>D06060G9_A9A8_1_CMV_Forward_C12_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNTGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG   121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT   181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT   241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC   301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA   361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA   421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG   481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA   541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA   601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA   661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG   721 CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA   781 GTTCGTGACC GCCGCCGGGN TCACTCTCGG CATGGNCGAN CTGTACAAGT CCGGANTCAG   841 ATCCATGTCG GANGGAGATT ACGATTANCT CATCAAGTTC TNNGCCTTNN NAGANTCNNG   901 GGNANGNNAN ANCANNNNAN NCTACNANNA CNNNGNTNGN NNGNTCNNCT CNNAATTCNT 961 CANNNNANNG GNNNNTGNTT TCNNNNNANN NNNNNNNN //
Primer: SV40pro_F_V2 Sequence file: RDB16889_A9A8d.seq
>D06060G9_A9A8_1_SV40pro_F_V2_D12_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCN NGGCTTTTGC NNAGNNCGAT CAAGAGACAG GATGAGGATC    61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT   601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA   721 TCANGACATA GCGTTGGCTA CNNGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA   781 NCGCTTCCTC GTGCTTTACG GTATCGCCGC TNCCGATTCG CAGCGCATCG CCTTCTATCG   841 CCTTCTTGAC GANTTCTTCT GANCGGGANT CTGGGGTTCG AAATGNNNNA NCNNNCGANG   901 CCCNNNCTGN CNTCACNANA TTNCNATTNC NCNGCCGNCN TCTATGNAAN NNNNGGGCTT 961 NNNNNNCNTT TTNCNGGGNN NNNNGNTNNN NGANNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl