Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-Rab27A-T23N

Expression vector of mouse Rab27A constitutive negative mutant, T23N, fused with EGFP at N-terminus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. and Kuroda, T.S. J. Biol. Chem. 277, 43096-43103, 2002)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. and Kuroda, T.S. J. Biol. Chem. 277, 43096-43103, 2002)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16888_A9A8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP 3', insert 5' (T23N) Sequence file: RDB16888_A9A8a.seq
>D06060G8_A9A8_2_EGFP-C2_E06_15_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNCN CNTGGTCCTG CTGGANTTCG TGANCGCCGC CGGGATCACT    61 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCA TGTCGGATGG AGATTACGAT   121 TACCTCATCA AGTTCTTGGC CTTGGGAGAC TCTGGGGTAG GGAAGAACAG TGTACTCTAC   181 CAGTACACTG ATGGCAAGTT CAACTCCAAA TTCATCACCA CAGTGGGCAT TGATTTCAGG   241 GAAAAGAGAG TGGTGTACAG AGCCAATGGG CCAGATGGAG CTGTGGGCCG AGGCCAGAGA   301 ATCCACCTGC AGTTATGGGA CACGGCGGGG CAGGAGAGGT TTCGGAGCTT AACCACTGCA   361 TTCTTCAGGG ACGCTATGGG TTTCCTGCTT CTGTTCGACC TGACAAATGA GCAAAGTTTC   421 CTCAATGTCC GAAACTGGAT AAGCCAGCTA CAGATGCACG CGTACTGTGA AAACCCAGAT   481 ATAGTGCTGT GTGGAAATAA GAGTGACCTG GAAGACCAGA GGGCAGTGAA AGAGGAGGAG   541 GCCCGGGAAC TTGCCGAGAA GTACGGAATC CCCTATTTTG AAACCAGTGC TGCCAACGGG   601 ACAAACATAA GCCACGCGAT TGAGATGCTC CTGGACCTGA TCATGAAGCG GATGGAGCGG   661 TGTGTGGACA AGTCCTGGAT TCCGGAGGGA GTGGTACGGT CCAACGGCCA TACCTCTGCG   721 GATCAGCTAA GTGAGGAGAA GGAGAAGGGG TTGTGTGGCT GTTGAAATCA CTAGTGAATT   781 CTGCAGTCGA CGGTACCGCG GGCCCGGGAN CCACCGGNNC TAGATAACTG ATCATAATCA   841 GCCNTACCAC ATTTGTAGAG GNTTTACTTG CTTTNAAAAA CCTCCCACAC NTCCCCCNGA   901 ANCTGAAACA TNAAATGANT GNNNNTGTTG TTGTTNACTT GNTTANTGNA GCTTNNANNG   961 GNTANNANTA AAGCNNNANC ATNNNNAATT TCNNNAATAA NNNNTTTTTT TNNNNNNNNN 1021 N //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB16888_A9A8b.seq
>D06060G8_A9A8_2_SV40polyA-R2_F11_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNAGTT AANNACNANN ATTGCATTCN TTTTATGTTT CNGGTTCAGG    61 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT   121 ATGATCAGTT ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC TGCAGAATTC   181 ACTAGTGATT TCAACAGCCA CACAACCCCT TCTCCTTCTC CTCACTTAGC TGATCCGCAG   241 AGGTATGGCC GTTGGACCGT ACCACTCCCT CCGGAATCCA GGACTTGTCC ACACACCGCT   301 CCATCCGCTT CATGATCAGG TCCAGGAGCA TCTCAATCGC GTGGCTTATG TTTGTCCCGT   361 TGGCAGCACT GGTTTCAAAA TAGGGGATTC CGTACTTCTC GGCAAGTTCC CGGGCCTCCT   421 CCTCTTTCAC TGCCCTCTGG TCTTCCAGGT CACTCTTATT TCCACACAGC ACTATATCTG   481 GGTTTTCACA GTACGCGTGC ATCTGTAGCT GGCTTATCCA GTTTCGGACA TTGAGGAAAC   541 TTTGCTCATT TGTCAGGTCG AACAGAAGCA GGAAACCCAT AGCGTCCCTG AAGAATGCAG   601 TGGTTAAGCT CCGAAACCTC TCCTGCCCCG CCGTGTCCCA TAACTGCAGG TGGANTCTCT   661 GGCCTCGGCC CACAGCTCCA TCTGGCCCAT TGGCTCTGTA CACCACTCTC TTTTCCCTGA   721 AATCAATGCC CACTGTGGTG ATGAATTTGG AGTTGAACTT GCCATCAGTG TACTGGTAGA   781 GTACACTGTT CTTCCCTACN NCAGANTCTC CCNANGCCAA GANNTTGATG ANGTAATCGT   841 AATCTCCATC CGANATGGAT CTGANNCCNG ANTTGTACAG CTCGNNNNTG NCGANANTGA   901 TNCCNGCGGC NGNCNCGANC TCCAGCNNNA NNNTGTGNTC GCNCTTNNCG NTGGGGTCTT   961 NGCNCNGGNC GGNNNNNNNN NTCNNNNANN NGNNNGNNGG NNANCANCNN GGGGNCNNNN 1021 NNNNNN //
Primer: CMV_Forward Sequence file: RDB16888_A9A8c.seq
>D06060G8_A9A8_2_CMV_Forward_G11_20_ABI24.ab1     1 NNNNNNNNNN NNNNTTGNNN CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC   121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA   181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG   241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC   301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC   361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC   421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC   481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG   541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG   601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC   661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC   721 ACCCAGTCCG CCCTGANCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAN   781 TTCGTGACCG CCGCCGGGNT CACTCTCGGC ATGNNCGANC TGTACAAGTC CGGANTCAGA   841 TCCNTGTCGG ANGGAGATTA CGATTACCTC ATCAAGTTCT NGGNCTNNGG ANANTCTGGG   901 GNANGGAAGA ANAGTGTACT CTANNANNAC NCNGATGGNN AGTTCNNCNN CNAATTCNTC 961 ACNNCNNNGG GNNNNNNNTN CNNGNNAANN ANNNNNNNNN N //
Primer: SV40pro_F_V2 Sequence file: RDB16888_A9A8d.seq
>D06060G8_A9A8_2_SV40pro_F_V2_H11_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGCTTTT GNAAGATCGA TCNAGAGACA GGANGAGGAT    61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA   121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC   181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA   241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG   301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC   361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG   421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA   481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC   541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA   601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG   661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGNCCG GCTGGGTGTG GCGGANCGCT   721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAANA GCTTGGCGGC GAATGGGCTG   781 ANCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCANCGCATC GCCTTCTATC   841 GCCTTCTTGA CGAGTTCTTC TGANCGGGNN TCTGGGGTTC GAAATGNCCG ACNNANCGAN   901 GCCCNACCNG NCNNNNCNAG NNTTCGANTC CNNNGNNNCC NTCNNTGNAA GGNNGGGCTT 961 NNGNNNNNTT TTNCNNGNNN NNCNGNNNNN NNGANNCNTN NNNNNNNNGN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl