Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-Rab12-T55N

Expression vector of mouse Rab12A constitutive negative mutant, T55N, fused with EGFP at N-terminus.

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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16881_A9A8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP 3',insert 5' Sequence file: RDB16881_A9A8a.seq
>D06060G1_A9A8_2_EGFP-C2_B05_02_ABI08.ab1     1 NNNNNNNNNN NNNNNCNCGN NCNCNTGGTC CTGCTGGANT TCGTGNCCGC CGCCGGGATC    61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CTATGGATCC CAGCGCCGCG   121 CTGCACCGAC GGCCCGCGGG CGGCAGCCTG GGCGCGGTCT CCCCGGCGCT GTCGGGCGGC   181 CAGGCCCGCC GGAGGAAGCA GCCCCCCAGG CCGGCCGACT TCAAGCTGCA GGTCATCATC   241 ATCGGCTCCC GCGGCGTGGG CAAGAACAGC CTGATGGAGC GCTTCACCGA CGACACGTTC   301 TGCGAGGCCT GCAAGTCCAC CGTGGGTGTT GACTTTAAAA TCAAGACTGT AGAACTAAGA   361 GGGAAGAAAA TTAGATTACA GATCTGGGAC ACAGCAGGGC AGGAGCGATT TAACAGCATT   421 ACCTCAGCCT ATTACAGAAG TGCCAAGGGG ATCATACTCG TATATGACAT CACCAAGAAG   481 GAGACGTTCG ATGACTTGCC AAAGTGGATG AAGATGATTG ACAAGTATGC TTCAGAAGAC   541 GCCGAGCTCC TCCTGGTTGG AAATAAGCTG GACTGTGAGA CAGACAGAGA AATCTCAAGG   601 CAGCAAGGAG AAAAGTTTGC ACAGCAGATA ACCGGGATGC GCTTCTGCGA AGCCAGTGCC   661 AAGGACAATT TCAATGTGGA CGAGATCTTT CTGAAACTCG TTGATGATAT TCTGAAAAAG   721 ATGCCTCTTG ATGTTTTGAG AAACGAGTTA TCCAATAGCA TCCTTTCTCT ACNACCAGAG   781 CCTGANATCC CACCAGANCT GCCTCCTCCC AGANCACACG TCCNATGCTG NTGATTTGAT   841 ACTTTGGAGA ATCACTANTG AATTCTGCAG NCGANNGTAN CGCGGGNCCN GGNTNCNNCG   901 GATCTAGATA NNTGATCATA ATCAGCNNTA CNNNATTTGT ANANGNTTTN CTTGCTTTAA   961 AAANNNNCCA CANNNCCCCN NGAANCNGAA ANATAAAATG NNNGNNNNNG NNGNNGNTNN  1021 CNTNGNTTNN NNNANCNNAN NANNNNNNNN ANNNNNNANN NCNNNNNNNN TNNNNNNNNN 1081 N //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB16881_A9A8b.seq
>D06060G1_A9A8_2_SV40polyA-R2_C05_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNANN NAANNANGNN NNNNNNAACA ATTGCATTCN TTTTATGTTT    61 CNGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT   121 ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC   181 TGCAGAATTC ACTAGTGATT CTCCAAAGTA TCAAATCAAC AGCATCGGAC GTGTGGTCTG   241 GGAGGAGGCA GCTCTGGTGG GATCTCAGGC TCTGGTTGTA GAGAAAGGAT GCTATTGGAT   301 AACTCGTTTC TCAAAACATC AAGAGGCATC TTTTTCAGAA TATCATCAAC GAGTTTCAGA   361 AAGATCTCGT CCACATTGAA ATTGTCCTTG GCACTGGCTT CGCAGAAGCG CATCCCGGTT   421 ATCTGCTGTG CAAACTTTTC TCCTTGCTGC CTTGAGATTT CTCTGTCTGT CTCACAGTCC   481 AGCTTATTTC CAACCAGGAG GAGCTCGGCG TCTTCTGAAG CATACTTGTC AATCATCTTC   541 ATCCACTTTG GCAAGTCATC GAACGTCTCC TTCTTGGTGA TGTCATATAC GAGTATGATC   601 CCCTTGGCAC TTCTGTAATA GGCTGAGGTA ATGCTGTTAA ATCGCTCCTG CCCTGCTGTG   661 TCCCAGATCT GTAATCTAAT TTTCTTCCCT CTTAGTTCTA CAGTCTTGAT TTTAAAGTCA   721 ACACCCACGG TGGACTTGCA GGCCTCGCAG AACGTGTCGT CNGTGAAGCG CTCCATCAGG   781 CTGTTCTTGC CCACGCCGCG GGANCCGANG ATGATGANCT GCAGCTTGAA GTCGGNCGGN   841 CTGGGGGGNT GCTTCCTCNN NNGGGCCTGG NCGCCCGANA GCGCCNGGGA GANCGCGCCC   901 AGGCTGNCGC CCGCGGGNCC NTCGGTGCAN CGCGGCGCTG GGANNNNTAN NTCTGANNCC   961 NGANTTGNAN AGCNNGTCCA TGCCNANNNT GATNCNNNCG GNNGNCACNA ACNNCANCAN 1021 NNNCNNGTGN TNNCNCNTNN NGNNGGGGNN TTNNNNCNNN NNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP 5' Sequence file: RDB16881_A9A8c.seq
>D06060G1_A9A8_2_CMV_Forward_D05_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTGNNNCAN NNGGGCGGTA GGCGTGTACG GTGGGAGGTC    61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA   121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG   181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG   241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC   301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC   361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT   421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG   481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA   541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG   601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG   661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT   721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC   781 TGCTGGAGTT CGTGACCGCC GCNNGGATCA CTCTCGGCAT GGNNGAGCTG TACAAGTCCG   841 GACTCAGATC TATGGATCCC AGCGCCGCGC TGCACCGNCN GNCCGCGGGC GGNANCCNNN   901 NCGCGGTCTC CCNGGCNCTG NCNGGNGGNC AGGCCCGCNN GNNGAANCAN CCCCCCCNNG   961 CNGGCCNANN NCNAGCTGNN NGNNNNTCNT CNTNGNNTCC NNNNNCGNGG NCAANAANNN 1021 NNCNNGANNG NANCGNNNNN NNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB16881_A9A8d.seq
>D06060G1_A9A8_2_SV40pro_F_V2_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CTTTTNNNNN NNCGATCAAG AGANAGGNTG AGGNTCGTTT    61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA   121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG   181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA   241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT   301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG   361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA   421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT   481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC   541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC   601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA   661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA NCGCTATCAG   721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGANCGC   781 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT   841 CTTGACGANT TCTTCTGANC GGGANTCTGG GGTTCGAAAT GACNGANCAA GCGACGCCCA   901 ACCTGCCATC ACGAGATTTC GANNCCACCG CCGCCTTCTA TGNNNNGNNG GGCTTCGGAN   961 TCGTTTTCCN GNNNGNCNGC TNNNTNNNNC NTNNNGCGCG GGGNTNNCNN GCNNNNNNNN 1021 NTNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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