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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab7-T22N

Expression vector of mouse Rab7A constitutive negative mutant, T22N, fused with EGFP at N-terminus.

Catalog number RDB16877
Resource name pEGFP-C1-Rab7-T22N
Clone info. Expression vector of mouse Rab7A constitutive negative mutant, T22N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab7 cDNA
Reference of insert sequence AB232599 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab7

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。
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          RDB16877 pEGFP-C1-Rab7-T22N DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEGFP-C1-Rab7-T22N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16877).

          Reference section:

          Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16877_A9A8p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP 3',insert 5'
          Sequence file: RDB16877_A9A8a.seq check
          >D06060I5_A9A8_2_EGFP-C2_A06_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCGNT CNCNTGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGNTCA
             61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGACCTCT AGGAAGAAAG
            121 TGTTGCTGAA GGTCATCATC CTGGGGGACT CTGGTGTTGG AAAGAACTCT CTCATGAACC
            181 AGTATGTGAA CAAGAAGTTC AGTAACCAGT ACAAAGCCAC AATAGGAGCG GACTTTCTGA
            241 CCAAGGAGGT GATGGTGGAC GACAGACTTG TTACCATGCA GATCTGGGAC ACAGCCGGTC
            301 AAGAACGGTT CCAGTCTCTT GGTGTGGCCT TCTACAGAGG TGCAGATTGC TGTGTTCTGG
            361 TGTTTGATGT GACTGCCCCC AACACTTTCA AAACCCTCGA CAGCTGGAGA GACGAGTTTC
            421 TCATCCAGGC CAGCCCCCGG GATCCCGAGA ACTTCCCTTT TGTTGTGTTG GGAAACAAGA
            481 TTGACCTGGA AAACAGACAA GTGGCCACAA AGAGGGCACA GGCTTGGTGC TACAGCAAAA
            541 ACAACATTCC TTACTTCGAG ACCAGTGCCA AGGAGGCCAT CAATGTGGAG CAGGCCTTCC
            601 AGACAATTGC TCGGAATGCC CTTAAACAGG AAACAGAAGT GGAACTGTAC AATGAATTCC
            661 CTGAACCCAT CAAACTGGAC AAGANTGACC GGGCCAAGGC CTCCGCAGAA AGCTGCAGTT
            721 GTTGAAATCA CTAGTGAATT CGCGGCCGCC TGCANGTCGA CGGTACNGCG GGCCCGGGNN
            781 NCACCGGNTC TAGATAACTG ATCATAATCA GCCNTACCAC ATTTGTAGAN GTTTTACTTG
            841 CTTTAAAAAA CCTCCCNCAC CTCCCCNNNN ANNNGAANNA TAAANNGNAN GNNNNTGNNG
            901 NNGNTNACNN GNTNANNNNN NCNNNNNNNG GNNANNANTN ANNCNNNNNC NTCNNNANTT
          961 TCNNNANNNA NNNNNTTTTT NNNNNNNNNN
          //
          Primer: SV40polyA_R2 (Pr0623)
          Region: insert 3'
          Sequence file: RDB16877_A9A8b.seq check
          >D06060I5_A9A8_2_SV40polyA-R2_B06_06_ABI24.ab1
              1 NNNNNNNNNC NNNNNNNNNN GTTAANNANN ACAATTGCAT TCNTTTTATG TTTCNGGTTC
             61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
            121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACCTGCAGG
            181 CGGCCGCGAA TTCACTAGTG ATTTCAACAA CTGCAGCTTT CTGCGGAGGC CTTGGCCCGG
            241 TCATTCTTGT CCAGTTTGAT GGGTTCAGGG AATTCATTGT ACAGTTCCAC TTCTGTTTCC
            301 TGTTTAAGGG CATTCCGAGC AATTGTCTGG AAGGCCTGCT CCACATTGAT GGCCTCCTTG
            361 GCACTGGTCT CGAAGTAAGG AATGTTGTTT TTGCTGTAGC ACCAAGCCTG TGCCCTCTTT
            421 GTGGCCACTT GTCTGTTTTC CAGGTCAATC TTGTTTCCCA ACACAACAAA AGGGAAGTTC
            481 TCGGGATCCC GGGGGCTGGC CTGGATGAGA AACTCGTCTC TCCAGCTGTC GAGGGTTTTG
            541 AAAGTGTTGG GGGCAGTCAC ATCAAACACC AGAACACAGC AATCTGCACC TCTGTAGAAG
            601 GCCACACCAA GAGACTGGAA CCGTTCTTGA CCGGCTGTGT CCCAGATCTG CATGGTAACA
            661 AGTCTGTCGT CCACCATCAC CTCCTTGGTC AGAAAGTCCG CTCCTATTGT GGCTTTGTAC
            721 TGGTTACTGA ACTTCTTGTT CACATACTGG TTCATGAGAG AGTTCTTTCC NACACCAGAN
            781 TCCCCCANGA TGATGANCTT CAGCAACACT TTCTTCCTAG ANGTCATGGA TCTGANTNCN
            841 GANTTGTACA GCTCGTCCAT GNCGAGANTG ATNCNNGCGG CGGNCACNAA CTNCNGCNNN
            901 ANNNTNTGNN CGCNCTTCNC NNNNGGGTCT TNGCNCNNGN CGGNNTGGGN GCTCNNNNAN
          961 NGGNTNNNCN GGNANCANCA CGGGGNCNNN CNNNNNNGGG GGGNNNNNNN NNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,EGFP 5'
          Sequence file: RDB16877_A9A8c.seq check
          >D06060I5_A9A8_2_CMV_Forward_C06_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNTTGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG
            121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
            181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
            241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
            301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
            361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
            421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
            481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
            541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
            601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
            661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN
            721 CACCCAGTCC GCCCTGANCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
            781 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAN CTGTACAAGT CCGGANTCAG
            841 ATCCNTGACC TCTANGAAGA AAGTGTTGCT GAAGGTCATC ATCCTGGGGG NNTNNGGNGT
            901 TGNAAAGAAC TCTCTCNTGA ACCAGNATGT GAANNANAAN TTCNGTANCC NNNACAANGC
          961 CNNNNNNNNA NNNNNNTTTN NGANNNNNNN NNN
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR_KanR
          Sequence file: RDB16877_A9A8d.seq check
          >D06060I5_A9A8_2_SV40pro_F_V2_D06_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGNN NNGGCTTTNG NNNGNNCGAT CNAGAGACAG GATGAGGNTC
             61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
            601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
            721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
            781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CANCGCATCG CCTTCTATCG
            841 CCTTCTTGAC GAGTTCTTCT GANCGGNANT CNGGGGGTTC GAAATGANCG ANCNNNCGAC
            901 GCCCAACCTG CCNTCACGAN ANTTCNATTC CNNNGCCNNN TTCTATGAAA NGNNTGGGCT
            961 TNNGNNTCGN TTTNCNGNNN NNCNNNNNGG ATGATNNTNN NNNNCNGGNN NNNNNNGNNN
          1021 NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.
          reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694.
          reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl