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pEGFP-C1-Rab5A-S34N

Expression vector of mouse Rab5A constitutive negative mutant, S34N, fused with EGFP at N-terminus.

Catalog number RDB16874
Resource name pEGFP-C1-Rab5A-S34N
Clone info. Expression vector of mouse Rab5A constitutive negative mutant, S34N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab5a cDNA
Reference of insert sequence AB232593 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab5a

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。
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          RDB16874 pEGFP-C1-Rab5A-S34N DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16874_A9A8p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP, insert 5'
          Sequence file: RDB16874_A9A8a.seq check
          >D06060F3_A9A8_2_EGFP-C2_E04_13_ABI24.ab1
              1 NNNNNNNNNN NNNNCNCGAT CNCNTGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
             61 CTCTCGGCAT GGNCGAGCTG TACAAGTCCG GACTCAGATC CATGGCTAAT CGAGGAGCAA
            121 CAAGACCCAA CGGGCCAAAT ACTGGAAATA AAATATGCCA GTTCAAACTG GTCCTTCTAG
            181 GAGAGTCCGC GGTTGGCAAA AACAGCCTGG TTCTTCGCTT TGTGAAAGGC CAATTTCATG
            241 AATTTCAAGA GAGTACCATT GGGGCTGCCT TTCTAACCCA AACTGTGTGT CTTGATGACA
            301 CAACAGTAAA ATTTGAAATA TGGGATACAG CTGGTCAAGA ACGGTATCAT AGCTTAGCAC
            361 CAATGTACTA CCGAGGAGCA CAAGCAGCCA TAGTTGTGTA TGATATCACA AATGAGGAAT
            421 CCTTTGCGAG AGCAAAAAAC TGGGTTAAAG AACTTCAAAG GCAAGCAAGT CCTAATATTG
            481 TGATAGCTTT GTCAGGAAAC AAAGCTGACT TAGCAAATAA AAGAGCTGTT GACTTCCAGG
            541 AAGCACAGTC CTATGCAGAT GACAACAGCT TATTATTTAT GGAGACATCA GCTAAGACAT
            601 CAATGAATGT AAATGAAATA TTTATGGCAA TAGCTAAAAA GCTGCCAAAG AATGAACCAC
            661 AGAATCCTGG TGCAAACTCA GCCAGAGGAC GANGAGTAGA CCTTACTGAG CCTGCACAGC
            721 CAGCCAGAAG CCAGTGTTGT AGTAACTGAA ATCGAATTCG ATATCAAGCT TATCGATACC
            781 GTCGACNGTA CCGCGGGCCC GGGNNCCACC GGATCTAGAT AACTGATCAT AATCAGCCNT
            841 ACCACATTTG TAGAGGNTTN ACTTGCTTTN AAAANNCTNC CNCACCNCCC CCNGAANCNG
            901 AAACNTNAAA TGNANGCNAT TGNTGNTGTT NNCNTGNTTA TTGNNNCTTA NNNNGGNTAN
          961 NAATNANGCN NNANCATCAN NAATTTNNCA AATAAAGNNN TTTTTTNNNN NNNNNN
          //
          Primer: SV40polyA_R2 (Pr0623)
          Region: insert 3'
          Sequence file: RDB16874_A9A8b.seq check
          >D06060F3_A9A8_2_SV40polyA-R2_F04_16_ABI24.ab1
              1 NNNNNNNNNC NNNNNNANNN NGTTAANNAN AACNATTGCA TTCNTTTTAT GTTTNNGGTT
             61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
            121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACGGTATC
            181 GATAAGCTTG ATATCGAATT CGATTTCAGT TACTACAACA CTGGCTTCTG GCTGGCTGTG
            241 CAGGCTCAGT AAGGTCTACT CCTCGTCCTC TGGCTGAGTT TGCACCAGGA TTCTGTGGTT
            301 CATTCTTTGG CAGCTTTTTA GCTATTGCCA TAAATATTTC ATTTACATTC ATTGATGTCT
            361 TAGCTGATGT CTCCATAAAT AATAAGCTGT TGTCATCTGC ATAGGACTGT GCTTCCTGGA
            421 AGTCAACAGC TCTTTTATTT GCTAAGTCAG CTTTGTTTCC TGACAAAGCT ATCACAATAT
            481 TAGGACTTGC TTGCCTTTGA AGTTCTTTAA CCCAGTTTTT TGCTCTCGCA AAGGATTCCT
            541 CATTTGTGAT ATCATACACA ACTATGGCTG CTTGTGCTCC TCGGTAGTAC ATTGGTGCTA
            601 AGCTATGATA CCGTTCTTGA CCAGCTGTAT CCCATATTTC AAATTTTACT GTTGTGTCAT
            661 CAAGACACAC AGTTTGGGTT AGAAAGGCAG CCCCAATGGT ACTCTCTTGA AATTCATGAA
            721 ATTGGCCTTT CACAAAGCGA AGAACCAGGC TGTTTTTGCC NACCGCGGAC TCTCCTAGAA
            781 GGANCAGTTT GAACTGGCAT ATTTTATTTC CAGTATTTGG CCCGTTGGGT CNTGTTGCTC
            841 CTCGATTANC CATGGNTCTG ANNNCGGANT TGTACAGCTC GTCCATGCCG ANANTGATNC
            901 NGGCGGCGGN CACGAACTCC ANCNNNANCN TGTGNNNGCN CTTCTCGTTG GGGNCNTTGN
            961 NCNNNNNNAN NNGNNGNTCN NNNANNNGNN NNNNNGGNAN CANCNNGGGN NNNNCNNNNN
          1021 NNGGGGNNNN NNNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, EGFP
          Sequence file: RDB16874_A9A8c.seq check
          >D06060F3_A9A8_2_CMV_Forward_G04_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG
            121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
            181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
            241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
            301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
            361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
            421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
            481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
            541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
            601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
            661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN
            721 CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
            781 GTTCGTGACC GCCGCCGGGN TCACTCTCGG CATGGNCGAN CTGTACAAGT CCGGANTCNN
            841 ATCCATGGCT AATCGAGGAN CAACAAGANC CAACGGGCCN AATACTGNAA ATAAAATATG
            901 NCAGTTCNAA CTGGNCCTTC NNGGANANNC CGNGGNNGGN AAAAACAGCC NNNNTNNNNC
          961 GCTTTGNGNA ANNNNNNNNT TCNTGNNNTN NNNNNNNNN
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR_KanR
          Sequence file: RDB16874_A9A8d.seq check
          >D06060F3_A9A8_2_SV40pro_F_V2_E04_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGCTTTTGCA AGNNCGATCN AGAGNNNGGN NGAGGNNCGT
             61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
            121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
            181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
            241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
            301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
            361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
            421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
            481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
            541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
            601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
            661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC
            721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGANCT TGGCGGCGAA TGGGCTGANC
            781 GCTTCCTCGT GCTTTACNGT ATCGCCGCTC NCGATTCGCA GCGCATCGCC TTCTATCGCC
            841 TTCTTGACNA NTTCTTCTGA NCGGGANTCT NGGGTTCGAA ATGNNNNANN NANCGACGCC
            901 NNACCNGCCN TCACNANANT TCGANNNCNN CNCCNNNNTC TATNAAANGN NNNGNNTTNN
          961 GNNNCGNTTT TNNNGGANGC NNNCNNGNNN NNNNCNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.
          reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694.
          reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

          2023.04.06

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