Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK407 (BSD-P2A-mAID-Nluc)

A plasmid for construction of N-terminal BSD-P2A-mAID-Nluc tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 付加的提供条件： 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16824_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 () Sequence file: RDB16824_A8Lpa.seq
>D06157C2_A8Lp_1_pUC-M13_-100_G08_20_ABI24.ab1     1 GGGCGGTAAA GCGATTAAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC    61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC   121 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGCCACC ATGGCCAAGC CTTTGTCTCA   181 AGAAGAATCC ACCCTCATTG AAAGAGCAAC GGCTACAATC AACAGCATCC CCATCTCTGA   241 AGACTACAGC GTCGCCAGCG CAGCTCTCTC TAGCGACGGC CGCATCTTCA CTGGTGTCAA   301 TGTATATCAT TTTACTGGGG GACCTTGTGC AGAACTCGTG GTGCTGGGCA CTGCTGCTGC   361 TGCGGCAGCT GGCAACCTGA CTTGTATCGT CGCGATCGGA AATGAGAACA GGGGCATCTT   421 GAGCCCCTGC GGACGGTGCC GACAGGTGCT TCTCGATCTG CATCCTGGGA TCAAAGCCAT   481 AGTGAAGGAC AGTGATGGAC AGCCGACGGC AGTTGGGATT CGTGAATTGC TGCCCTCTGG   541 TTATGTGTGG GAGGGCTCAG GAAGCGGAGC TACTAACTTC AGCCTGCTGA AGCAGGCTGG   601 AGACGTGGAG GAGAACCCTG GACCTAGATC TAAGGAGAAG AGTGCTTGTC CTAAAGATCC   661 AGCCAAACCT CCGGCCAAGG CACAAGTTGT GGGATGGCCA CCGGTGAGAT CATACCGGAA   721 GAACGTGATG GTTTCCTGCC AAAAATCAAG CGGTGGCCCG GAGGCGGCGG CGTTCGTGAA   781 AGGTATCAAT GGACGGAGCA CCGTACTTGA GGAAAATCGA TTTGAGGATG TATAAAGCTA   841 GCATGGTCTT CACACTCGAA GATTTCGTTG GGGGACTGGC GACAGACAGC CGGCTACAAC   901 CCTGGACCAA GTCCTTGAAA CAGGGAGGTG TGTCCAGTTT GTTTTCAGAA TCTCGGGGTG   961 TCCGTAACTC CGATCCCAAA GATTGTCCCT GAGCGGTGAA AAATGGGCTG AAGATCGACA  1021 TCCAATGTCA TCATTCCTCG TATGACGTCT GAGCGGCCGA ACAATGGCCA GATCGAAATT 1081 TTAAGTTGGT GTTAACCCCT GGATG //
Primer: reverse () Sequence file: RDB16824_A8Lpb.seq
>D06157C2_A8Lp_1_reverse_H08_23_ABI24.ab1     1 AAAGCCAATT AACCCTTCAC TAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC    61 GCTCTAGAAC TAGTGGATCC GGCGCCGGCC CCTGCACCAG CGCCCGCCAG AATGCGTTCG   121 CACAGCCGCC AGCCGGTCAC TCCGTTGATG GTTACTCGGA ACAGCAGGGA GCCGTCGGGG   181 TTGATCAGGC GCTCGTCGAT AATTTTGTTG CCGTTCCACA GGGTCCCTGT TACAGTGATC   241 TTTTTGCCGT CGAACACGGC GATGCCTTCA TACGGCCGTC CGAAATAGTC GATCATGTTC   301 GGCGTAACCC CGTCGATTAC CAGTGTGCCA TAGTGCAGGA TCACCTTAAA GTGATGATCA   361 TCCACAGGGT ACACCACCTT AAAAATTTTT TCGATCTGGC CCATTTGGTC GCCGCTCAGA   421 CCTTCATACG GGATGATGAC ATGGATGTCG ATCTTCAGCC CATTTTCACC GCTCAGGACA   481 ATCCTTTGGA TCGGAGTTAC GGACACCCCG AGATTCTGAA ACAAACTGGA CACACCTCCC   541 TGTTCAAGGA CTTGGTCCAG GTTGTAGCCG GCTGTCTGTC GCCAGTCCCC AACGAAATCT   601 TCGAGTGTGA AGACCATGCT AGCTTTATAC ATCCTCAAAT CGATTTTCCT CAAGTACGGT   661 GCTCCGTCCA TTGATACCTT CACGAACGCC GCCGCCTCCG GGCCACCGCT TGATTTTTGG   721 CAGGAAACCA TCACGTTCTT CCGGTATGAT CTCACCGGTG GCCATCCCAC AACTTGTGCC   781 TTGGCCGGAG GTTTGGCTGG ATCTTTAGGA CAAGCACTCT TCTCCTTAGA TCTAGGTCCA   841 GGGTTCTCCT CCACGTCTCC AGCCTGCTTC AGCAGCTGAA GTTAGTAGCT CCGCTTCCCT   901 GAGCCCTCCC ACACATAAAC CAGAGGGCAG CAATTCACGA ATCCCCAACT GCCCGTCGCT   961 GTCCATCACT GTCCTTTCAC TATGGCCTTG GATCCCAAGG ATGCCAGATC GGAGAAGCAC  1021 CCTGGTCGTC CACGTTCCGG CAGGGCTTCA GGAATGGCCC CCCTTGGTTC TCAATTCCCG 1081 ATTCGCGAAC GATACCAAGT TCCAGGGATT TCGGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.10.05

GNP_filter3_RDBDEP_html_231001.pl