Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK388 (mClover-BSD)

A plasmid for construction of C-terminal mClover-BSD tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16816_A8Lpp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,insert 5'(Linker,mClover 5') Sequence file: RDB16816_A8Lpe.seq
>D06157B3_A8LP_2_pUC-M13_-100_A06_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGNANGCNN GGNNTTTCCC NGTCNCGACG TTGTAAAACG    61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CGGATCCGGT   121 GCAGGCGCCG CTAGCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC   181 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTCCGCGG CGAGGGCGAG   241 GGCGATGCCA CCAACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC   301 GTGCCCTGGC CCACCCTCGT GACCACCTTC GGCTACGGCG TGGCCTGCTT CAGCCGCTAC   361 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG   421 GAGCGCACCA TCTCTTTCAA GGACGACGGT ACCTACAAGA CCCGCGCCGA GGTGAAGTTC   481 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC   541 AACATCCTGG GGCACAAGCT GGAGTACAAC TTCAACAGCC ACAACGTCTA TATCACGGCC   601 GACAAGCAGA AGAACGGCAT CAAGGCTAAC TTCAAGATCC GCCACAACGT TGAGGACGGC   661 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACNG CCCCGTGCTG   721 CTGCCCGACA ACCACTACCT GANCCATCAG TCCAAGCTGA NCAAAGANCC CAACGANAAN   781 CGCGATCACA TGGNCCTGCT GGNNTTCGTG ANCGCCGCCG GGANTACACA TGGNATGGNN   841 GANCTGTNCN AGTAATCTAG ATANNTGATC ATAATCAGCC NTACCACATT TGNANNNGNT   901 TNNNTNGCTT NAAAAAAANC NNCCNCNNNN NCCCNNNNAN NNNNNANNNN NANNNNNNNN 961 GNNNNNNNNN N //
Primer: reverse (Pr0099) Region: insert 3'(mPGK ter,BSD) Sequence file: RDB16816_A8Lpf.seq
>D06157B3_A8LP_2_reverse_B06_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN ANGNNNNNAA GCTGGGTANC GGNTCCTAGT GAACCTCTTC    61 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG GGTTCCGGAT   121 CTCGACCAGC TTCTGATGGA ATTAGAACTT GGCAAAACAA TACTGAGAAT GAAGTGTATG   181 TGGAACAGAG GCTGCTGATC TCGTTCTTCA GGCTATGAAA CTGACACATT TGGAAACCAC   241 AGTACTTAGA ACCACAAAGT GGGAATCAAG AGAAAAACAA TGATCCCACG AGAGATCTAT   301 AGATCTATAG ATCATGAGTG GGAGGAATGA GCTGGCCCTT AATTTGGTTT TGCTTGTTTA   361 AATTATGATA TCCAACTATG AAACATTATC ATAAAGCAAT AGTAAAGAGC CTTCAGTAAA   421 GAGCAGGCAT TTATCTAATC CCACCCCACC CCCACCCCCG TAGCTCCAAT CCTTCCATTC   481 AAAATGTAGG TACTCTGTTC TCACCCTTCT TAACAAAGTA TGACAGGAAA AACTTCCATT   541 TTAGTGGACA TCTTTATTGT TTAATAGATC ATCAATTTCT GCATCCCGGG GATCTGATAT   601 CATCGATTAG CCCTCCCACA CATAACCAGA GGGCAGCAAT TCACGAATCC CAACTGCCGT   661 CGGCTGTCCA TCACTGTCCT TCACTATGGC TTTGATCCCA GGATGCAGAT CGAGAAGCAC   721 CTGTCGGCAC CGTCCGCAGG GGCTCAAGAT GCCCCTGTTC TCATTTCCGA TCGCGACGAT   781 ACAAGTCANG TTGCCNGCTG CCGCANCAGC ANCAGTGCCC AGCACCACGA GTTCTGCACA   841 AGGTCCCCCC AGNNAAATGA TATACATTGA CACCNGNGAA GATGCGGNNG TCNCNNNANA 901 NANCNNCGNN NGNNANNCNN NANNCNNCNN NNATGGGNNA NGNNNNNNNN NN //
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP,SV40 pA,mClover 3') Sequence file: RDB16816_A8Lpg.seq
>D06157B3_A8LP_2_PGK_N_rev_C06_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNC NGGNANANTT CGAGGTCGAG NTCCTAAGCT TGGCTGGACG    61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG   121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA   181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA   241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG   301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG   361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCATG TGTAATCCCG   421 GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC   481 AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG   541 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC GTTGTGGCGG   601 ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA GACGTTGTGG   661 CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG   721 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC   781 TTGTANGTAC CGTCGTCCTT NNNAGAGATG GTGCGCTCCT GGACGTANCC TTCGGGCATG   841 GCGGANTTGA AGAANTCGNG CTGCTTCATG TGGNCNGGGN ANCGGCNGNN NCANGCCACG   901 CCNNANCCNA NNNNGGNCAC NANGGNNGGN CAGGNNNNGG GCNNCTNNNN NNNNGNNNNN 961 NNNNN //
Primer: PGKpro_F (Pr0249) Region: insert mid(mPGK ter,BSD) Sequence file: RDB16816_A8Lph.seq
>D06157B3_A8LP_2_PGKpro_F_D06_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTN NNNNTCTCCG GGCCTTTCGA NCTGCATCCN TCTAGATCTC    61 GAGCCACCAT GGCCNAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG   121 CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA   181 GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG   241 AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG   301 CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC   361 TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG   421 TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAATCG ATGATATCAG   481 ATCCCCGGGA TGCAGAAATT GATGATCTAT TAAACAATAA AGATGTCCAC TAAAATGGAA   541 GTTTTTCCTG TCATACTTTG TTAAGAAGGG TGAGAACAGA GTACCTACAT TTTGAATGGA   601 AGGATTGGAG CTACGGGGGT GGGGGTGGGG TGGGATTAGA TAAATGCCTG CTCTTTACTG   661 AAGGCTCTTT ACTATTGCTT TATGATAATG TTTCATAGTT GGATATCATA ATTTAAACAA   721 GCAAAACCAA ATTAAGGGNC AGCTCATTCC TCCCACTCAT GATCTATAGA TCTATAGATC   781 TCTCGTGNGA TCATTGTTTT TCTCTTGATT CNNACTTTGT GGTTCTAAGT ACTGTGGTTT   841 CCAAATGTGT CAGTTTCATA GCCNGAANAA CGAGATCAGC ANCCTCNGTT CCNCNTACNC   901 TTCNTTCTCA GTATTGNTTT TGNNNNNNNC NAANNNCNTC ANAAGCNNGN NCGANATNNN 961 NNNANNNNTN NNNNNNNNTT NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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