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pCMV-eMvChR1#2-Venus

Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2).

Catalog number RDB16788
Resource name pCMV-eMvChR1#2-Venus
Clone info. Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter.
Comment eMvChR1#2 consists of Met1-Ala37 of channelrhodopsin 2 from Chlamydomonas reinhardtii (CrChR2) and His57-Arg323 of channelrhodopsin 1 from Mesostigma viride (MvChR1). Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone pVenus-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Chlamydomonas reinhardtii acop2 (cop4) cDNA Mesostigma viride MvChR1 cDNA
Depositor|Developer Ishizuka, Toru |
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External Database
Chlamydomonas reinhardtii acop2 (cop4) Mesostigma viride MvChR1

          Reference sequence
            AB058891,AF461397,JF922293
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, S. et al., Photochem. Photobiol. Sci., 15: 365-374, 2016) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Watanabe, S. et al., Photochem. Photobiol. Sci., 15: 365-374, 2016)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB16788 pCMV-eMvChR1#2-Venus Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pCMV-eMvChR1#2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16788).

          Reference section:

          Watanabe, S., Ishizuka, T., Hososhima, S., Zamani, A., Hoque, M.R., Yawo, H., The regulatory mechanism of ion permeation through a channelrhodopsin derived from Mesostigma viride (MvChR1). Photochem. Photobiol. Sci. 15 (3): 365-374 (2016). PMID 26853505. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Watanabe, S., The regulatory mechanism of ion permeation through a channelrhodopsin derived from Mesostigma viride (MvChR1). Photochem. Photobiol. Sci. 15 (3): 365-374 (2016). PMID 26853505. [link to RRC of NBRP]

          2023.11.23

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