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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA-Ostir1-pA

Expression vector of transport inhibitor response 1 derived from Oryza sativa (OsTIR).

Catalog number RDB16762
Resource name pcDNA-Ostir1-pA
Clone info. Expression vector of transport inhibitor response 1 derived from Oryza sativa (OsTIR) mRNA synthesis. Auxin-inducible degron (AID) system clone for in vitro transcription, which has an extended poly (A) sequence.
Comment Expression was confirmed by the depositor with EGFP monitoring by microinjectioning its mRNA with hPLCz-AID-EGFP mRNA into mouse oocytes and culturing them in the presence of indole-3-acetic acid.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Oryza sativa OsTIR1 cDNA
Depositor|Developer Ogura, Atsuo |
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External Database
Oryza sativa OsTIR1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018) is requested.
          Additional terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Miura, K. et al. J. reprod Dev. 64 (4): 319-326, 2018) の引用を必要とする。
          付加的提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishimura, K., Nat. Methods., 6, 917-922 (2009))。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB16762 pcDNA-Ostir1-pA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Miura, K., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16762_A8K6p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB16762_A8K6a.seq check
          >D06043A2_A8K6_2_CMV-Forward_E06_15_ABI24.ab1
              1 AGGTTCCCTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
            121 ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTTCG AATTATGACG TACTTCCCGG
            181 AGGAGGTGGT GGAGCACATC TTCAGCTTCC TGCCGGCGCA GCGCGACCGC AACACGGTCT
            241 CGCTCGTCTG CAAGGTGTGG TACGAGATCG AGAGGCTGAG CCGCCGCGGC GTCTTCGTGG
            301 GCAACTGCTA CGCCGTGCGC GCCGGCCGCG TCGCCGCGCG GTTCCCCAAC GTGCGGGCGC
            361 TCACGGTGAA GGGGAAGCCC CACTTCGCCG ACTTCAACCT CGTGCCCCCC GACTGGGGCG
            421 GCTACGCGGG GCCGTGGATC GAGGCGGCCG CGAGGGGATG CCACGGCCTG GAGGAGCTCA
            481 GGATGAAGCG GATGGTGGTG TCCGACGAGA GCCTCGAGCT GCTGGCTCGC TCGTTCCCGC
            541 GGTTCAGGGC TCTTGTTCTT ATCAGCTGCG AGGGGTTCAG CACTGACGGG CTAGCCGCCG
            601 TCGCGAGCCA TTGCAAGCTT CTGAGGGAGT TGGATTTGCA GGAAAATGAA GTGGAGGATC
            661 GAGGGCCTAG TGGCTTTCCT GCTTCCCTGA TTCCTGCACA TCACTGTCTC ATGATTTTGC
            721 CTGCATCAAA GGGGAGGTAT GCTGTCCACT GGAGAGACTT GTTAGCAGGT CCCCAAACTG
            781 GCGGAGTTTG AGGCTGATCG ATCTTGTATC GTAGAATACA CTTGCAAGAT ACTACTGCGT
            841 ACCCCTACTG GAGGATTGGG GAATGGGTAT TTGGACGGAT GACTTCAACC TGAATCCTCA
            901 TTTAAGCTAC CAGGCCTGGA ATGCCAATGT GAGATGCTGG AATCCGGACC CTCCGATGCC
          961 GTCATATATA C
          //
          Primer: GNP_Primer#10
          Sequence file: RDB16762_A8K6b.seq check
          >D06043A2_A8K6_2_GNP_Primer#10_F06_18_ABI24.ab1
              1 TAATTTGTGC AAATTCAATT TAAAAGATAA CGTTTACAGG TTCTTTGGTG CCCATAGTCG
             61 AGCGGCCTTA ACCACTAGCA GCAGAACCGG AGTCGACTAG GATTTTAACA AAATTTGGTG
            121 CATCATCCCT CGCCCCAGCA GTTGTGCGGT ACACATATAA TTTCTCCACT TTAGGCAGAT
            181 CTCCATGGTT TTCCTCCATT TCATTGCTAC CATCCCGCTC ATTTATGACC TCAACATTGA
            241 GCATCGGCAT CTTTGACGCA AGGACTTGGC ACCCCTTTAA CGTGACATTG CAAGATGACA
            301 TCCAAAGGGA TCGCATTGTC TCGTACCTAG CAAAATTCCC CAAGAGTGCA GCATCACCAA
            361 ACGGGCTATC TCTTATCTCC AGTTTCCTTA AATTCTTGCA TCCATTCATA ACATGCATCA
            421 TACCCTTATC ACTGTCACCA GCAAATGCTA TAGAAAGCAT CTCAAGTTGT TTTGCATATT
            481 TCCCAATATA CATGAAAACT TTGTCTGTGA GAAGACCAGA TATTGACAAA CGTTGTAATC
            541 CCTTGCACTC ACGAACAATA GCTCCAAAGC CTTCATCTAA TGGTTGGCTT GTCACAACAT
            601 CAGGCTTCCC TGGCTCAAGA ATACAAAGTC TGAATCGTGT GAAATTTGGA CAGTTCTTGG
            661 CGACAGTAAC TAGTGCAGCA TTAGTCATTT GGTGACAGAA GTACAGTAGT GAGTTCAGTT
            721 TTGGACAGCC CAAGGATACT GCAACAAGTC CCTCCTCTGT CACTGCAGAA TAACCAGCTA
            781 CGTAGAAATC TGATGGAAAT ACCCTGAGTT CTTGCAAGTC TTTGCAACTG GAGGCCACCA
            841 CTTGCAAGCC TTTGTCCGAG ATACAATCCA GTACCCAAAG GCGTTGGAGC TTCACACAGC
            901 GGCTAATCAT TTTTTGTAAG GTCAGAAGGC ATCAAAGTGT GGGGTGCATA GCCTCAAGTT
            961 CAATCCTGTC AGTTGAGCAA CACAAGGGGG GTAGAATAAA TGACAGGCAA ACCAGGAGAA
           1021 GCATCCAGAA TCAGACAACT CCTCAACCAT CCTGGCATTT CTCAGAGACA CTTGGTAGCC
          1081 TTTAAAGTTA GGACTCAGTT GGAAGTCAAT CCTGG
          //
          Primer: SV40pro_F
          Sequence file: RDB16762_A8K6c.seq check
          >D06043A2_A8K6_2_SV40pro_F_G06_21_ABI24.ab1
              1 CCTGGTCCCT GGGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
             61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
            121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
            661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
            781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
            841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
            901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAAGC
            961 GACGCCCAAC CCTGCCCATC ACGAGATTTC GATTTCCACC GCCGCCTTCT ATGAAGGTTG
           1021 GGCTTTCGGA ATCGGTTTTC CGGGACGCCG CTGGAATGGA TTCTTCAAGC GCGAGCATCT
          1081 CATGCTGGAA GCTCATCGCC CAACCCCAAC CTTTGGTCCA T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Miura, K., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl