RDB16708 - GFP-OPTN-E50K

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GFP-OPTN-E50K

Expression vector of human optineurin (OPTN), amyotrophic lateral sclerosis (ALS)-associated E50K mutant.

Catalog number	RDB16708
Resource name	GFP-OPTN-E50K
Clone info.	Expression vector of human optineurin (OPTN), amyotrophic lateral sclerosis (ALS)-associated E50K mutant, fused with enhanced monomeric EGFP at N-terminal, CMV promoter.
Comment	Expression was confirmed by the deposior with fluorescence image.
Vector backbone	pmEGFP-C1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name	human OPTN cDNA
Depositor\|Developer	Kinjo, Masataka \|

Other clones in our bank

human OPTN

External Database
human OPTN

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16708	GFP-OPTN-E50K	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Original reference

original	Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB16708_A8Ilp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: VenusC_F2 () Sequence file: RDB16708_A8Ila.seq
>D05635A2_A8Il__1_VenusC_F2_B01_02_ABI08.ab1 1 GGGAGTGGTA AAGCGCCGGG AACACTCTCG GCATGGACGA GCTGTACAAG TCCGGACTCA 61 GATCTCGAGC TCAAGCTTCG AATTCTATGT CCCATCAACC TCTCAGCTGC CTCACTGAAA 121 AGGAGGACAG CCCCAGTGAA AGCACAGGAA ATGGACCCCC CCACCTGGCC CACCCAAACC 181 TGGACACGTT TACCCCGGAG GAGCTGCTGC AGCAGATGAA AGAGCTCCTG ACCAAGAACC 241 ACCAGCTGAA AGAAGCCATG AAGCTAAATA ATCAAGCCAT GAAAGGGAGA TTTGAGGAGC 301 TTTCGGCCTG GACAGAGAAA CAGAAGGAAG AACGCCAGTT TTTTGAGATA CAGAGCAAAG 361 AAGCAAAAGA GCGTCTAATG GCCTTGAGTC ATGAGAATGA GAAATTGAAG GAAGAGCTTG 421 GAAAACTAAA AGGGAAATCA GAAAGGTCAT CTGAGGACCC CACTGATGAC TCCAGGCTTC 481 CCAGGGCCGA AGCGGAGCAG GAAAAGGACC AGCTCAGGAC CCAGGTGGTG AGGCTACAAG 541 CAGAGAAGGC AGACCTGTTG GGCATCGTGT CTGAACTGCA GCTCAAGCTG AACTCCAGCG 601 GCTCCTCAGA AGATTCCTTT GTTGAAATTA GGATGGCTGA AGGAGAAGCA GAAGGGTCAG 661 TAAAAGAAAT CAAGCATAGT CCTGGGCCCA CGAGAACAGT CTCCACTGGC ACGGCATTGT 721 CTAAATATAG GAGCAGATCT GCAGATGGGG CCAAGAATTA CTTCGAACAT GAGGAGTTAA 781 CTGTGAGCCA GCTCCTGCTG TGCCTAAGGG AAGGGAATCA GAAGGTGGAG AGACTTGAAG 841 TTGCACTCAA GGAGGCCAAA GAAAGAGTTT CAGATTTTGA AAAGAAAACA AGTAATCGTT 901 CTGAGATTGA AACCCAGACA GAGGGGAGCA CAGAGAAAGA GAATGATGAA GAGAAAGGCC 961 CGGGAGACTG TTGGGAAGCG AAGTGAAGCA CTGAACCCTC CCAGGTGACA TCTCTGTTTA 1021 GAGCTTTCAG GAGGCTCATA CAAACTCAGC GAAGCTGAAG CCTATGGAGG AGAGACTTCC 1081 AAGATAGTGT CAGGTCCGTG CAAGAAAACT TCCTGCCATT TCCCATTCAG GAG //
Primer: SV40polyA-R () Sequence file: RDB16708_A8Ilb.seq
>D05635A2-1_A8Il_SV40polyA-R_E01_13_ABI24.ab1 1 AAGAAGGGGG AAGTTTTTTA AAGCAGTAAA CCTCTACAAA TGTGGTATGG CTGATTATGA 61 TCAGTTATCT AGATCCGGTG GATCCCGAAT GATGCAATCC ATCACGTGAA TCTGTAACGT 121 GTCTATGTCA GGCAGAACCT CTCCACACTT GGGGCAGGAA TGAATCGGAA TATTCCGCTG 181 TTGCCGCCAG TCCCTGTCCT CAGCTCCTCT TTGAACAAGG TAAGCCTGCT GGTCAGAGTC 241 ACTTGTTCTC GCCCCATGAC GACTCTGCAT CTCCATCAAG GACTGCCTGC CTCCGTCTTC 301 GAAAGCATCA TTCTCTTTCA GCAGAACTGC CAGCTGCAAT GCCAGTTGCT CCTTTTCCTC 361 ATGAATTTTC TCTCTCGCTG CTCTTTCAGC ATGAAAATCA GAACAGTAAA CTTCCATCTG 421 AGCCCTGAGG ATGGTCATGG TTTCCAGGTC CTCTTCCTGC TTGGCAATGG TTTGCTTCAT 481 TTCATCCATT TGCAGCTGTT TGGAAGCCAG AGCCTTCTCT GCCAGTTCCA GTTTTTCACT 541 CAGTTCCTTC AGCACTGCCC TGTCCACTTT TTCTGACTCT TTTCTTGTTA GTTCCTCAAT 601 TGTTTTCAAT GCATTATTAT GTTCTTGAAG AAGCTTGTTG TGTGTCATCT GTAGCACAGT 661 TAATTTGGAC TTTTCATCCT CTGTTTTAGC CTGTTCCATT TTGATTTCTG ATAGCATGCT 721 TTCCACTTGT AGCTCTAACT TTTTGTTAGT ATAAACAAGC TCTTGCTTTT CATTCAACTC 781 TGATGGAATT GCAGAATTTT TCCTTTCAAG GGCCTGACAC TTTTCTTGAA GTCTCTTCTT 841 CATTAGCTCA GCTTCGCTGA GTTTTGTATG AGCCTCTTGA AGCTCCTTAA ACAGAGATGT 901 CACCTGGAGG TTCAGTGCTT CCACTTCGCT TCCAACAGTC TCCGGGCCTT TCTCTTCATC 961 ATTCTCTTTC TCTGTGCTCC CCTCTGTCTG GGTTTCAATC TCAGAACGAT TACTTTGTTT 1021 TCTTTCAAAA TCTGAAACTC TTCTTTGGGC CTCTTGAGTG CAACTTTCAA GTCTCTCCAC 1081 CTTCTGATCC TTTCCTTAGG CAACAGCAGG AAGCTGTCTC ACAGTAACTT CCTCATGATC 1141 CGAAGGTATT CTTTGGGCCC CAATCTGTCA GAGAAATCCT G //
Primer: SV40pro_F_V2 () Sequence file: RDB16708_A8Ilc.seq
>D05635A2-1_A8Il_SV40pro_F_V2_F01_16_ABI24.ab1 1 AAAGGAGGTT TTTTTGGGAG GCCTAGGCTT TTGCAAGATC GATCAAGAGA CAGGATGAGG 61 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG 601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 841 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG 901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGG 961 CTTCGGAATC GTTTTCCGGG ACGCCCGGCT GGATGATCCC TCCAGCGCGG GGGATCTCAT 1021 GCTGGGAGTT CTTCGCCCAC CCTAGGGGGA GGCTAACTGA AACACGAAGA GAACATACCG 1081 GGAAGGACCC GCGCTATGAC GGGCATAAAA GACGGATAAC GCCACGTGTG GGTTCGGATT 1141 GTCATTAAAC GCCGGGATTC CGGTTCCCCA AG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153.

2023.04.06

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