Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-SB11-IRES-puro

Retroviral expression vector of fish Sleeping Beauty 11 (SB11) transposase in mammalian cells.

Distribution information

Ordering	This clone is provided directly from Dr. Hideto Koso of the Institute of Medical Science, Tokyo University.

提供案内 (日本国内) [open/close]

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16060_A8Idp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F2 Sequence file: RDB16060_A8Ida.seq
>D05854A1-2_A8Id_MMLV-pol_F2_F02_17_ABI24.ab1     1 TCGAGGGGGG GTTTGAATTC CTTCTAGACT GCCGGATCTA GCTAGTTAAT TAAGGATCCA    61 CCATGGGAAA ATCAAAAGAA ATCAGCCAAG ACCTCAGAAA AAAAATTGTA GACCTCCACA   121 AGTCTGGTTC ATCCTTGGGA GCAATTTCCA AACGCCTGAA AGTACCACGT TCATCTGTAC   181 AAACAATAGT ACGCAAGTAT AAACACCATG GGACCACGCA GCCGTCATAC CGCTCAGGAA   241 GGAGACGCGT TCTGTCTCCT AGAGATGAAC GTACTTTGGT GCGAAAAGTG CAAATCAATC   301 CCAGAACAAC AGCAAAGGAC CTTGTGAAGA TGCTGGAGGA AACAGGTACA AAAGTATCTA   361 TATCCACAGT AAAACGAGTC CTATATCGAC ATAACCTGAA AGGCCGCTCA GCAAGGAAGA   421 AGCCACTGCT CCAAAACCGA CATAAGAAAG CCAGACTACG GTTTGCAAGA GCACATGGGG   481 ACAAAGATCG TACTTTTTGG AGAAATGTCC TCTGGTCTGA TGAAACAAAA ATAGAACTGT   541 TTGGCCATAA TGACCATCGT TATGTTTGGA GGAAGAAGGG GGAGGCTTGC AAGCCGAAGA   601 ACACCATCCC AACCGTGAAG CACGGGGGTG GCAGCATCAT GTTGTGGGGG TGCTTTGCTG   661 CAGGAGGGAC TGGTGCACTT CACAAAATAG ATGGCATCAT GAGGAAGGAA AATTATGTGG   721 ATATATTGAA GCAACATCTC AAGACATCAG TCAGGAAGTT AAAGCTTGGT CGCAAATGGG   781 TCTTCCAACA AGACAATGAC CCCAAGCATA CTTCCAAACA CGTGAGAAAA TGGCTTAAGG   841 ACAACAAAGT CAAAGGTATT GGAGTGGCCA TCACAAAGCC CTGACCTCAA TCCTATAGAA   901 AATTTGTGGG CAGAACTGAA AAAAGCGTGT GCGAGCAAAG GAGGCCTACA AACCCTGACT   961 CAGTTTACAC CCAGCTCTGT CAGGAGAAAT GGGCCCAAAT TCACCCAACT TATGTGGAAG  1021 CTTGTGGCAA GGCTACCGAA CCGTTTGACC AAGTCGACCA ATTTAAAGTC AATGCTACCT 1081 AAT //
Primer: CSII-CMV3' Sequence file: RDB16060_A8Idb.seq
>D05854A1-2_A8Id_CSII-CMV3'_G02_20_ABI24.ab1     1 GGGCGGATAG TAGCGGCCGC GGCGCGCCGG CCCTCGAGGC CTGCAGGAAT TCGCCCTTCT    61 AGTATTTGGT AGCATTGCCT TTAAATTGTT TAACTTGGGT CAAACGTTTC GGGTAGCCTT   121 CCACAAGCTT CCCACAATAA GTTGGGTGAA TTTTGGCCCA TTCCTCCTGA CAGAGCTGGT   181 GTAACTGAGT CAGGTTTGTA GGCCTCCTTG CTCGCACACG CTTTTTCAGT TCTGCCCACA   241 AATTTTCTAT AGGATTGAGG TCAGGGCTTT GTGATGGCCA CTCCAATACC TTGACTTTGT   301 TGTCCTTAAG CCATTTTCTC ACGTGTTTGG AAGTATGCTT GGGGTCATTG TCTTGTTGGA   361 AGACCCATTT GCGACCAAGC TTTAACTTCC TGACTGATGT CTTGAGATGT TGCTTCAATA   421 TATCCACATA ATTTTCCTTC CTCATGATGC CATCTATTTT GTGAAGTGCA CCAGTCCCTC   481 CTGCAGCAAA GCACCCCCAC AACATGATGC TGCCACCCCC GTGCTTCACG GTTGGGATGG   541 TGTTCTTCGG CTTGCAAGCC TCCCCCTTCT TCCTCCAAAC ATAACGATGG TCATTATGGC   601 CAAACAGTTC TATTTTTGTT TCATCAGACC AGAGGACATT TCTCCAAAAA GTACGATCTT   661 TGTCCCCATG TGCTCTTGCA AACCGTAGTC TGGCTTTCTT ATGTCGGTTT TGGAGCAGTG   721 GCTTCTTCCT TGCTGAGCGG CCTTTCAGGT TATGTCGATA TAGGACTCGT TTTACTGTGG   781 ATATAGATAC TTTTGTACCT GTTTCCTCCA GCATCTTCAC AAGGTCCTTT GCTGTTGTTC   841 TGGGATTGAT TTGCACTTTT CGCACCAAAG TACGTTCATC TCTAGGAGAC AGAACGCGTC   901 TCCTTCCTGA GCGGTATGAC GGCTGCGTGG TCCCATGGGT GTTTATACTT GCGTACTATT   961 GTTTGTACAG ATGAAACGTG GTACTTTCAG GCGTTTGGGA AAATTGGCTC CCAACGATGG  1021 AACCAGACTT GTTGAGTCTA CAATTTTTTT TCTGAAGTCC TGCTGGATTT TCCTTTTGAA 1081 TTTTCCCC //
Primer: IRES-F Sequence file: RDB16060_A8Idc.seq
>D05854A1-2_A8Id_IRES-F_H02_23_ABI24.ab1     1 GCAGCTTTCA TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC    61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGGTTA CCGAGTACAA   121 GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC   181 CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG   241 GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG   301 GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG   361 GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC   421 GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT   481 GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT   541 CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC   601 CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC   661 CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGAGTCG ACGATAAAAT   721 AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG   781 GCAAGCTAGC TTAAGTAACG CCATTTTGCA AAGGCATGGA AAAATACATA ACTGAGAATA   841 GAGAAGTTCA GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC CAAACAGGAT   901 ATCTGTGGGT AAGCAGTTTC CTGCCCCGGG CTCATGGCCC AAGAAACAGA TGGACAGCTG   961 ATATGGGCCA AACCAGATAT TCTGTGGTAG CAGTTCTTGC CTCACTCAAG ACAGACAGAT 1021 GATCCCCAGA TGCCGATCAG TCTCAGTCAG ATTCTAGAAC CTATCAGATG GATA //
Primer: PuroR_F Sequence file: RDB16060_A8Idd.seq
>D05854A1-2_A8Id_PuroR_F_C01_03_ABI08.ab1     1 TTGCGTTTTC GGCCGGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC    61 AAGCCCGGTG CCTGAGTCGA CGATAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG   121 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA   181 GGCATGGAAA AATACATAAC TGAGAATAGA GAAGTTCAGA TCAAGGTCAG GAACAGATGG   241 AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG   301 GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC   361 CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC CTCAGCAGTT   421 TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT   481 ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC   541 TCAATAAAAG AGCCCACAAC CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC   601 CCGGGTACCC GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG   661 TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC TTTCACATGC   721 AGCATGTATC AAAATTAATT TGGTTTTTTT TCTTAAGTAT TTACATTAAA TGGCCATAGT   781 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG   841 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC   901 ACTCAAAGGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG   961 TGAGCAAAAG GCCAGCAAAA GGCCAGGGAA CCCGTAAAAA GGCCGCGTTG CTGGGCGTTT  1021 TTTCCATAGG CTCCGCCCCC CCTGACGAGC ATCACAAAAA AATCGACGCT CAAGGTCAGA  1081 GTGGCGAAAC CCGAACAAGG GACTTATAAG AATACCAGGC GTTTCCCCTG AGCTCCATCG 1141 GTGGCGCTTC TCTTGTTCCG GACTCTGTGC CGGCCTTAAC CGGAAATACC CCTGTGATC //
Primer: Amp_R Sequence file: RDB16060_A8Ide.seq
>D05854A1-2_A8Id_Amp_R_A01_01_ABI08.ab1     1 GCTTTTTTTT CATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA    61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG   121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT   181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA   241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG   301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA   361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC   421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT   481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC   541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT   601 GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT   661 CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT   721 CATTTGGGGG CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG   781 GGAGGTAAGC TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA   841 TTTTATGCGC CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG   901 TGGAACTGAC GAGTTCGGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTCGG   961 GGGCCGTTTT TGTGGCCCGA CCTGAGTCCA AAAATCCCGA TCGTTTTGAC TCTTTGGGTG  1021 CACCCCCCTA ATAGGAGGGA TATGTGGTTC TGGTAGGAGA CGAGAACCTA AAACAGTTCC  1081 CGCCTCCGTC TGAATTTTTG CTTTCGGTTG GACCGAAGCG CGTCGCGCGA TCCTGGTCTG 1141 GCTGCCAGCC ATCGATTCGT GATGATGGAT CTCCCTGGTT CCTGGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl