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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCX-mSlit2

Expression vector of mouse slit guidance ligand 2 (Slit2) with a signal sequence from the human CD8A gene, the FLAG peptide and the 6xHis tag.

Catalog number RDB16025
Resource name pCX-mSlit2
Clone info. Expression vector of mouse slit guidance ligand 2 (Slit2) in pCX vector containing a kozak sequence, a signal sequence from the human CD8A gene, the FLAG peptide, and the 6xHis tag.
Comment Expression was confirmed by the depositor with fluorescece imaging.
Vector backbone pCX (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name mouse Slit2 cDNA
Depositor|Developer Masu, Masayuki |
Other clones in our bank
External Database
mouse Slit2
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 7(1), 13847, 2017). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Tsukuba University. (chizai@ilc.tsukuba.ac.jp) RECIPIENT must contact the DEPOSITOR (chizai@ilc.tsukuba.ac.jp) in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 7(1), 13847, 2017)。 非営利機関が非営利目的の教育・研究用に用いる場合以外は、筑波大学と別途MTAを締結すること(chizai@ilc.tsukuba.ac.jp)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者(chizai@ilc.tsukuba.ac.jp) と別途協議を行うこと。
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          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16025 pCX-mSlit2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCX-mSlit2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16025).

          Reference section:

          Okada, T., Keino-Masu, K., Nagamine, S., Kametani, F., Ohto, T., Hasegawa, M., van Kuppevelt, T.H., Kunita, S., Takahashi, S., Masu, M., Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation. Sci. Rep. 7 (1): 13847 (2017). PMID 29062064.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16025_A8Htp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F_V3 (Pr0495)
          Region: signal seq,FLAG,6xHis,insert 5'
          Sequence file: RDB16025_A8Htc.seq check
          >D05660A8-3_A8Ht_pCAG-F_V3_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGGNNNNN GGNANGGCGG GGTTCGGCTT CTGGCGTGTG
             61 ANCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACNGCTC
            121 CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CGCGCCTGCA
            181 GGCCACCATG GCCTTACCAG TGACCGCCTT GCTCCTGCCG CTAGCCTTGC TGCTCCACGC
            241 CGCCAGGCCG GATTACAAGG ACGATGACGA CAAGCATCAT CACCACCATC ACTCTGCAGG
            301 CGGCCGCCAG GCGTGCCCGG CCCAGTGCTC CTGTTCAGGC AGCACGGTGG ACTGTCATGG
            361 GCTGGCACTG CGCAGTGTGC CCAGGAATAT CCCCCGCAAC ACCGAGAGAC TGGATTTGAA
            421 TGGAAATAAC ATCACGAGGA TCACGAAGAT AGATTTTGCT GGTCTCAGGC ACCTCAGAGT
            481 TCTTCAGCTC ATGGAGAACA GAATCAGCAC CATCGAGAGG GGAGCATTCC AGGATCTTAA
            541 GGAGCTGGAA AGACTGCGTT TAAACAGAAA TAACCTTCAG TTGTTTCCTG AGCTGCTGTT
            601 TCTCGGGACT GCGAAGCTCT ACCGGCTTGA TCTCAGTGAA AATCAAATTC AAGCAATTCC
            661 AAGGAAGGCT TTCCGTGGGG CAGTTGACAT TAAAAACCTG CAACTGGATT ACAACCAGAT
            721 CANCTGCATT GAAGATGGGG CGTTCAGAGC TCTACGANAT CTGGAAGTGC TCACTCTGAA
            781 CNATAACAAT ATTACTAGAC TTTCAGTGGC AAGTTTCAAC CATATGCCNN AAACTNAGGA
            841 CATTTCGACT CCACTCNAAN NAACTTGTAC TGCGNNTGCC ACCTANCCTG GCTCTCANAC
            901 TGGCTTCGNC NAAGGNCACG GGNGGGCTNG TACACTCANN NNNTGGGNCC NNTNCNNNCC
          961 NGANGNNNNA NNATGNNNNN NANGNNTNNA ANANNNANNN NTTNNNNNNN NNN
          //
          Primer: pCAGGS_R1 (Pr0614)
          Region: beta-globin pA,insert 3'
          Sequence file: RDB16025_A8Htd.seq check
          >16025_16025_B1JdF_pCAGGS_R1_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNCNA TTGNAATGAA AATAAATTTC CTTTATTAGC CAGAAGTCAG
             61 ATGCTCAAGG GGCTTCATGA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCTCAGTG
            121 GTATTTGTGA GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC
            181 CTGAGGAGTG AATTCGCGCG GTACCAGTGT ACAACCTTCG CCGAAGCTAG AAGCTTCTAG
            241 AGACGCGCTT AGGAGGCACA TCTCGCGCAG CCGCACTTCA CCACTTTCTC AACCTCGTCC
            301 ACAAATGAGG AGCCATCTGT GCATTCGAAA GAGTATTTCC GCCTCTTGCT TCTCAGAGGT
            361 CCACAGCACT GGCCTCCAGC GCACCCGCCT CTGCATTCCA AGCGAGATAC TTTCTTAGTT
            421 GTTTGACAGG CAGCGTAACC CTGCTGCTTC TGGTAATAGT CCCTTATCCG TTCCCCTCGA
            481 CAAGAAATTT CTCTATCACA GCTGTCCCCG GTGAATCCAC TGTTGCATTC ACAATAGGGC
            541 TGGCCCACTC CAGAAAGCCT GCACTTCCCA TGCTTGCACT TGATCATCTG GCANGGGTTA
            601 AAGAGATCTT CTTCTTCATC ACAGAGGACA CCGCCATGGN CCTCCNGGCA CTTGCNACTA
            661 TAGGAGAANG NNTTGATGGG CNGGNNNNNC CNNNGNNNNC NTTTGTTTNC NANGNNGNNN
          721 CNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Okada, T., Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation. Sci. Rep. 7 (1): 13847 (2017). PMID 29062064.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl