Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHisGST CKI homolog 1

Bacterial expression vector of S. cerevisiae CKI homolog 1, residues 62-355.

Catalog number RDB15965
Resource name pHisGST CKI homolog 1
Clone info. Bacterial expression vector of S. cerevisiae CKI homolog 1, residues 62-355, fused with fused with His-GST at N terminus.
Comment Expression was confirmed by the depositor.
Vector backbone pET-28a(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name S. cerevisiae CKI homolog 1 cDNA
Depositor|Developer Ueda, Hiroki R. |
Other clones in our bank

External Database
S. cerevisiae CKI homolog 1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shinohara, Y et al. Molecular Cell 67, 783–798, 2017)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shinohara, Y et al. Molecular Cell 67, 783–798, 2017)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15965 pHisGST CKI homolog 1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Shinohara, Y., Temperature-Sensitive Substrate and Product Binding Underlie Temperature-Compensated Phosphorylation in the Clock. Mol. Cell 67 (5): 783-798.e20 (2017). PMID 28886336.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15965_A8Ehp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: GST-M_F
          Sequence file: RDB15965_A8Eha.seq check
          >D05351A2_A8Eh_2_GST-M_F_D08_11_ABI24.ab1
              1 GCCGGTGGGA CGTTTGAAGC TCGTCAAGTT GATTTTCTTA GCGAAGCTAC CTGAAATGCT
             61 GAAAATGTTC GAAGATCGTT TATGTCATAA AACATATTTA AATGGTGATC ATGTAACCCA
            121 TCCTGACTTC ATGTTGTATG ACGCTCTTGA TGTTGTTTTA TACATGGACC CAATGTGCCT
            181 GGATGCGTTC CCAAAATTAG TTTGTTTTAA AAAACGTATT GAAGCTATCC CACAAATTGA
            241 TAAGTACTTG AAATCCAGCA AGTATATAGC ATGGCCTTTG CAGGGCTGGC AAGCCACGTT
            301 TGGTGGTGGC GACCATCCTC CAAAATCGGA TGGGTCTAGC GGCTCCAGCG AACACCTGTA
            361 TTTTCAGGGA TCCACTATTG TCGGCCTACA TTACAAGATC GGCAAAAAAA TAGGGGAAGG
            421 TTCCTTTGGT GTGCTATTTG AAGGTACTAA TATGATCAAT GGCGTACCCG TCGCGATCAA
            481 ATTCGAGCCC AGAAAAACGG AGGCCCCTCA ATTAAGAGAT GAATATAAAA CATATAAAAT
            541 TCTGAATGGC ACTCCCAATA TCCCCTACGC GTACTACTTC GGCCAAGAAG GTTTGCACAA
            601 TATCTTGGTC ATTGATCTTT TGGGTCCCTC TTTGGAAGAT TTATTTGATT GGTGTGGAAG
            661 AAAATTTTCT GTCAAAACGG TTGTGCAAGT TGCTGTCCAA ATGATTACTT TGATTGAAGA
            721 CTTGCACGCA CATGACTTGA TATACCGTGA TATCAAACCA GACAATTTCT TGATTGGAAG
            781 GCCCGGCCAA CCTGACGCAA ACAACATCCA TTTGATCGAC TTCGGTATGG CCAAACAGTA
            841 TCGTGATCCG AAAACTAAAC AGCACATCCC ATATAGAGAG AAAAAAATCA CTCAGCGGCA
            901 CTGCCAGATA TATGTCCATT AATACTCACC TTGGAAGAGA GCAGTCCAGA AGGAGATGAT
            961 ATGGAGGGCC CTTGGGGTCA CGTTTTTCTT TTATTTCTTG AGAGGGCCCA CTTACCCTGG
           1021 CAGGATTTAA AAAGCTCCCA AACCATAAAG CCAAAAAATA CGAATAAGAC TGGCTGCACA
          1081 GGCAAAGATC CTACTTAATC GTTTTTTA
          //
          Primer: f1ori_R3
          Sequence file: RDB15965_A8Ehb.seq check
          >D05351A2_A8Eh_2_f1ori-R3_E08_14_ABI24.ab1
              1 AGCATCCCAT CGGATTAGTT CCTCCTTTCA GCAAAACCCC TCAAGACCCG TTTAGAGGCC
             61 CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG
            121 GCTTTGTTAG CAGCCGGATC TCAGTGGTGG TGGTGGTGGT GCTCGAGTTA CAGTTTCATC
            181 CAATCATATT GGCCGTCCGC GGTTTCACCT AAATCATCCA GTACAGATAG TAATAGTTTT
            241 CTATAGCCTT CATAATCGGG ACACTCTTCA AAGGAAAGAC TTCTGACGAT TTCTAGATAC
            301 CTGCCAAATT GCACAGGTAA GCCTTGAGCT AGATCGTAAA CGTTAGTAGA TCTTTTCTTT
            361 TCACCAATCT TTTCGTATTT TTGCTTATTG TTTGGAGCTT TTAAACCCTG CCAGGGTAAG
            421 TGGCCTCTCA AGAAATAAAA GAAAACGTGA CCCAAGGCCT CCATATCATC TCTTCTGGAC
            481 TGCTCTCTTC CAAGGTGAGT ATTAATGGAC ATATATCTGG CAGTGCCGCT GAGTGATTTT
            541 TTCTCTCTAT ATGGGATGTG CTGTTTAGTT TTCGGATCAC GATACTGTTT GGCCATACCG
            601 AAGTCGATCA AATGGATGTT GTTTGCGTCA GGTTGGCCGG GCCTTCCAAT CAAGAAATTG
            661 TCTGGTTTGA TATCACGGTA TATCAAGTCA TGTGCGTGCA AGTCTTCAAT CAAAGTAATC
            721 ATTTGGACAG CAACTTGCAC AACCGTTTTG ACAGAAAATT TTCTTCCACA CCAATCAAAT
            781 AAATCTTCCA AAGAGGGACC CAAAAGATCA ATGACCAAGA TATTGTGCAA ACCTTCTTGG
            841 CCGAAGTAGT ACGCGTAGGG GATATTGGGA GTGCCATTCA GAATTTTATA TGTTTTATAT
            901 TCATCTCTTA ATTTGAGGGC CTCCGTTTTT CTGGGCTCGA AATTTGATCG CGACGGGTAC
            961 GCCATTGATC ATATTAGTAC CTTCAAATAG CACACCCAAG GAACCTTTCC CCTATTTTTT
           1021 TTTGCCCGAT CTGTTAATGT AGCCCGACAT AGTGGCATCC CTGAAATACA GTGATCGCTG
          1081 GGAGCCGCTA GACCCATCCC GGAAGT
          //
          Primer: GST-N_rev
          Sequence file: RDB15965_A8Ehc.seq check
          >D05351A2_A8Eh_2_GST-N_rev_F08_17_ABI24.ab1
              1 TATCCTTCAG GATTTTTCCA AAAGAAGTCG GAGTGGGTTG CACAAGGCCC TTAATTTTCC
             61 AATAACCTAG TATAGGGGAC ATGGCATGAG CGTGCTCATG TTTGTGATGA TTGTGGATGA
            121 GATGATCTTT GCCCATGGTA TATCTCCTTC TTAAAGTTAA ACAAAATTAT TTCTAGAGGG
            181 GAATTGTTAT CCGCTCACAA TTCCCCTATA GTGAGTCGTA TTAATTTCGC GGGATCGAGA
            241 TCTCGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG
            301 CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC TTCGGGCTCA
            361 TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA CTGTTGGGCG
            421 CCATCTCCTT GCATGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC AACCTACTAC
            481 TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG TCGAGATCCC GGACACCATC
            541 GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG
            601 GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT
            661 CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA
            721 GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG
            781 GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG
            841 CAAATTGTCG CGGGCGATTA AATCTCGCGC CGATCAACTG GGTGCCAGCG TGGTGGTGTC
            901 GATGGTAGAA CGAAGCGGGC GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTTCTCGCG
            961 CAACGCGTCA GTGGGGCTGA TCATTAACTA TCCGCTGGAT GACCAGGATG CCATTTGCCT
           1021 GTGGAAAGCT GCCCTGCAAC TAATGTTTCC GGCGTTTATT CCTTGGAATG TCTCCTGACC
          1081 CAGACACTCC ATTCCACCAG GTAAATTTAA TTTTTTCCTT GCCAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Shinohara, Y., Temperature-Sensitive Substrate and Product Binding Underlie Temperature-Compensated Phosphorylation in the Clock. Mol. Cell 67 (5): 783-798.e20 (2017). PMID 28886336.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl