RDB15881 - pGEX6P-3-SUV39H1(39-105)-4A

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pGEX6P-3-SUV39H1(39-105)-4A

Expression vector of mouse SUV39H1, chromodomain (residues 39-105), nucleic acid-binding defective mutant (R84A, K87A, K91A, R95A).

Catalog number	RDB15881
Resource name	pGEX6P-3-SUV39H1(39-105)-4A
Clone info.	Expression vector of mouse suppressor of variegation 3-9 homolog 1 (SUV39H1), chromodomain (CD, residues 39-105), nucleic acid-binding defective mutant (R84A, K87A, K91A, R95A) fused with N-terminal GST, tac promoter, see Fig. 1A and Fig. 2A of Shirai, A. et al., Elife, 1; 6. pii: e25317, 2017.
Comment	Expression was confirmed by the depositor with CBB staining and Western blot.
Vector backbone	pGEX-6P-3 (plasmid)
Size of vector backbone	5.0 kb
Selectable markers	Amp^r
Gene/insert name	mouse Suv39h1 cDNA
Depositor\|Developer	Shinkai, Yoichi \|

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mouse Suv39h1

External Database
mouse Suv39h1

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shirai, A et al. ELIFE. (6), e25317, 2017)

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shirai, A et al. ELIFE. (6), e25317, 2017)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shirai, A et al. ELIFE. (6), e25317, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15881	pGEX6P-3-SUV39H1(39-105)-4A	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15881_A8Dgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_F2 Sequence file: RDB15881_A8Dga.seq
>D05294A2-A8Dg__2_pGEX_F2_G02_07_ABI08.ab1 1 GCATTTAGGT TTGGTTTTAA AAACGTATTG AAGCTATCCC ACAAAATTGA TAAGTACTTG 61 AAATCCAGCA AGTATATAGC ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC 121 GACCATCCTC CAAAATCGGA TCTGGAAGTT CTGTTCCAGG GGCCCCTGGG ATCCAATCTG 181 TATGACTTTG AAGTTGAATA CCTGTGTGAT TATAAGAAGA TCCGTGAGCA GGAGTATTAC 241 CTGGTTAAGT GGCGTGGGTA TCCCGACTCA GAAAACACCT GGGAGCCACG GCAGAATCTA 301 AAATGTATAG CAGTTCTTGC ACAGTTCCAC GCAGACTTAG AAGCAGAGCT TGTCCGACGA 361 CACCGCCGGT CAAAGTGAGT CGACTCGAGC GGCCGCATCG TGACTGACTG ACGATCTGCC 421 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 481 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 541 TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATAATT 601 CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 661 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 721 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 781 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 841 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 901 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 961 GTAAGATCCT TGAGAGTTTT CGCCCCGGAA GAACGTTTTC CCAATGATGA GCACTTTTAA 1021 AGTTTCTGCT ATGTGGCGCG GGTATTAATC CCGTGTTTGA CGCCCGGGCA AGAGCAACTC 1081 GGGTCGCCGC ATACACTATT TCTCAGAATG ACTGGTGGAA GTTACTTCAC CCAGTTCAAC 1141 AGAAAAAGGC AATTCCTTAA CCGGAATGTG GGC //
Primer: pGEX-ptac_up-F Sequence file: RDB15881_A8Dgb.seq
>D05294A2-2_A8Dg_pGEX-ptac_up-F_C01_03_ABI08.ab1 1 ATATTGGCGG GAATAAAAAC GGGTTCTGGG CAATATTCTG AAATGAGCTG TTGACAATTA 61 ATCATCGGCT CGTATAATGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG 121 TATTCATGTC CCCTATACTA GGTTATTGGA AAATTAAGGG CCTTGTGCAA CCCACTCGAC 181 TTCTTTTGGA ATATCTTGAA GAAAAATATG AAGAGCATTT GTATGAGCGC GATGAAGGTG 241 ATAAATGGCG AAACAAAAAG TTTGAATTGG GTTTGGAGTT TCCCAATCTT CCTTATTATA 301 TTGATGGTGA TGTTAAATTA ACACAGTCTA TGGCCATCAT ACGTTATATA GCTGACAAGC 361 ACAACATGTT GGGTGGTTGT CCAAAAGAGC GTGCAGAGAT TTCAATGCTT GAAGGAGCGG 421 TTTTGGATAT TAGATACGGT GTTTCGAGAA TTGCATATAG TAAAGACTTT GAAACTCTCA 481 AAGTTGATTT TCTTAGCAAG CTACCTGAAA TGCTGAAAAT GTTCGAAGAT CGTTTATGTC 541 ATAAAACATA TTTAAATGGT GATCATGTAA CCCATCCTGA CTTCATGTTG TATGACGCTC 601 TTGATGTTGT TTTATACATG GACCCAATGT GCCTGGATGC GTTCCCAAAA TTAGTTTGTT 661 TTAAAAAACG TATTGAAGCT ATCCCACAAA TTGATAAGTA CTTGAAATCC AGCAAGTATA 721 TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG TGGCGACCAT CCTCCAAAAT 781 CGGATCTGGA AGTTCTGTTC CAGGGGCCCC TGGGATCCAA TCTGTATGAC TTTGAAGTTG 841 AATACCTGTG TGATTATAAG AAGATCCGTG AGCAGGAGTA TTACCTGGTT TAAGTGGCGT 901 GGGTATCCCG ACTCAGAAAA CACCTGGGAG CCACGGCAGA ATCTAAAATG TATAGCAGTT 961 CTTTGCACAG TTCCACGCAG ACTTAAGAAG CAGAGCTTTG TTCCGACGAC ACCGCCCGGT 1021 TCAAAGTGAG TCGACTCGAG CGGCCCGGCA TCGGTGAACC TGGACTGACC GATCTGCCTC 1081 GCGCGATTCC GGTTGAATGA ACCGGCTGGA AAAACCCTTC TTGAACCACA ATTGCCAA //
Primer: SV40pA3' element Reverse Sequence file: RDB15881_A8Dgc.seq
>D05294A2-2_A8Dg_SV40pA3'elementReverse_D01_04_ABI08.ab1 1 GAGTCTTTGG GGGGGCGGGG GGCTATGGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT 61 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG 121 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG 181 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA 241 CGCATCTGTG CGGTATTTCA CACCGCATAA ATTCCGACAC CATCGAATGG CGCAAAACCT 301 TTCGCGGTAT GGCATGATAG CGCCCGGAAG AGAGTCAATT CAGGGTGGTG AATGTGAAAC 361 CAGTAACGTT ATACGATGTC GCAGAGTATG CCGGTGTCTC TTATCAGACC GTTTCCCGCG 421 TGGTGAACCA GGCCAGCCAC GTTTCTGCGA AAACGCGGGA AAAAGTGGAA GCGGCGATGG 481 CGGAGCTGAA TTACATTCCC AACCGCGTGG CACAACAACT GGCGGGCAAA CAGTCGTTGC 541 TGATTGGCGT TGCCACCTCC AGTCTGGCCC TGCACGCGCC GTCGCAAATT GTCGCGGCGA 601 TTAAATCTCG CGCCGATCAA CTGGGTGCCA GCGTGGTGGT GTCGATGGTA GAACGAAGCG 661 GCGTCGAAGC CTGTAAAGCG GCGGTGCACA ATCTTCTCGC GCAACGCGTC AGTGGGCTGA 721 TCATTAACTA TCCGCTGGAT GACCAGGATG CCATTGCTGT GGAAGCTGCC TGCACTAATG 781 TTCCGGCGTT ATTTCTTGAT GTCTCTGACC AGACACCCAT CAAACAGTAT TATTTTCTCC 841 CATGAAGACG GGTACGCGAC TGGGGCGTGG GAGCATCTGG TCGCATTGGG TCACCAGCAA 901 AATCGCGCTG TTAGCGGGGC CCCATTAAGT TCTGGTCTCG GCGCGTCTGC GTCTGGCCTG 961 GGCTGGCATA AAATATCTCA CTCGCCAATC GAAATTCAGC CGATAGCGGA ACGGGGGAAG 1021 GGCGACTGGG ATTGCCATGT TCCCGGGTTT CACCAAAACC AATGCCAAAA TGGCTGGAAT 1081 TGAGGCCCAT CGTTTCCAAC TTGGCGAATG CCTTGGGTTG CTCCAAGACG CGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl