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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-6p-2-3MST

E. coli expression vector of mouse 3-mercaptopyruvate sulfurtransferase (3MST).

Catalog number RDB15868
Resource name pGEX-6p-2-3MST
Clone info. E. coli expression vector of mouse 3-mercaptopyruvate sulfurtransferase (3MST, Mpst) fused with GST at N-terminal.
Comment Note: nucleotide substitution in mouse Mpst (A to G in 499nt of NM_138670.3 (Asp to Gly)), which has been noticed by the depositor.
Vector backbone pGEX-6P-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Mouse Mpst cDNA
Depositor|Developer Kimura, Hideo |
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External Database
Mouse Mpst

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature designated by the DEPOSITOR are requested (Kimura, Y et al. Sci. Rep. 7(1), 10459, 2017). Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kimura, Y et al. Sci Rep. 7(1), 10459, 2017)を引用し、謝辞の表明を必要とする。営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB15868 pGEX-6p-2-3MST DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kimura, Y., 3-Mercaptopyruvate sulfurtransferase produces potential redox regulators cysteine- and glutathione-persulfide (Cys-SSH and GSSH) together with signaling molecules H2S2, H2S3 and H2S. Sci. Rep. 7 (1): 10459 (2017). PMID 28874874.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15868_A8Cbp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Sequence file: RDB15868_A8Cba.seq check
          1
          Primer: pGEX3'
          Sequence file: RDB15868_A8Cbb.seq check
          1
          Primer: SV40pA3' element Reverse
          Sequence file: RDB15868_A8Cbc.seq check
          1
          Primer: pGEX-ptac_up-F
          Sequence file: RDB15868_A8Cbd.seq check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kimura, Y., 3-Mercaptopyruvate sulfurtransferase produces potential redox regulators cysteine- and glutathione-persulfide (Cys-SSH and GSSH) together with signaling molecules H2S2, H2S3 and H2S. Sci. Rep. 7 (1): 10459 (2017). PMID 28874874.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl