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pMRB

Expression vector of mCherry and ble under the control of Perkinsus marinus MOE gene promoter.

Catalog number RDB15791
Resource name pMRB
Clone info. Bicistronic expression vector of mCherry and Sh-ble intervened by a self-cleaving 2A peptide, which is controled under the Perkinsus marinus MOE (PmMOE) promoter.
This clone is for a drug selection system using bleomycin for transfecting Perkinsus species. Perkinsus is sensitive to bleomycin, and 100 microgram/ml of this drug completely blocks its proliferation.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pCR4-TOPO (Plasmid)
Size of vector backbone 4 kb
Selectable markers Amp^r, Kan^r.
Gene/insert name Perkinsus marinus MOE genomic DNA Discoma sp. RFP cDNA Streptoalloteichus hindustanus bleomycin resistance gene genomic DNA
Depositor|Developer Nozaki, Tomoyoshi | Matsuzaki, Motomichi |
Other clones in our bank
External Database
Perkinsus marinus MOE
    Discoma sp. RFP
      Streptoalloteichus hindustanus bleomycin resistance gene
            Reference sequence
              
             
            Sequence RDB15791z.seq

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of one of the literature designated by the DEPOSITOR is requested (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定するいずれかの文献を引用する (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press)。
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            RDB15791 pMRB Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pMRB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15791).

            Reference section:

            Sakamoto, H., Kita, K., Matsuzaki, M., A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes. J. Eukaryot. Microbiol. 68 (5): e12861 (2021). PMID 34051022. [link to RRC of NBRP]
            Sakamoto, H., Hirakawa, Y., Ishida, K., Keeling, P.J., Kita, K., Matsuzaki, M., Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 69: 13-16 (2018). PMID 30389616. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Sakamoto, H., A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes. J. Eukaryot. Microbiol. 68 (5): e12861 (2021). PMID 34051022. [link to RRC of NBRP]
            original Sakamoto, H., Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 69: 13-16 (2018). PMID 30389616. [link to RRC of NBRP]

            2023.12.13

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