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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

ptol2/cmlc2-mermaid

Voltage indicator Mermaid.

Catalog number RDB15780
Resource name ptol2/cmlc2-mermaid
Clone info. Voltage indicator Mermaid.
Vector backbone pT2KXIG delta in (Plasmid)
Gene/insert name Ciona intestinalis Ci-VSP cDNA Sarcophyton sp. GFP (Umikinoko-green) cDNA Fungia concinna OFP (Kusabira Orange) cDNA
Depositor|Developer Miyawaki, Atsushi |
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            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Tsutsui H, Higashijima SI, Miyawaki A, Okamura Y. (2010) Visualizing voltage dynamics in zebrafish heart. J. Physiol., 588 (Pt 12): 2017-2021.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Additional terms and conditions:
            The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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            MTA, for use for not-for-profit academic purpose [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Tsutsui H, Higashijima SI, Miyawaki A, Okamura Y. (2010) Visualizing voltage dynamics in zebrafish heart. J. Physiol., 588 (Pt 12): 2017-2021.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            付加的提供条件:
            Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15780 ptol2/cmlc2-mermaid DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The ptol2/cmlc2-mermaid was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15780).

            Reference section:

            Tsutsui, H., Higashijima, S., Miyawaki, A., Okamura, Y., Visualizing voltage dynamics in zebrafish heart. J. Physiol. 588 (Pt 12): 2017-2021 (2010). PMID 20421282. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


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            Featured content

            Featured content Sensor and Visualization (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15780_A8D8p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: cmlc2pro_F (Pr0643)
            Region: insert mid(cmlc2 pro,Mermaid 5')
            Sequence file: RDB15780_A8D8f.seq check
            >D04498J8_A8D8_3_cmlc2pro_F_A02_02_ABI24.ab1
                1 NNNNNNNNNN NNNNNAGAGT CCTCNTACNT AAAGACTCCN TTAGTAAGCC AGTGACCCAG
               61 GAGCCCAGAC CAACAGCAAA GCAGACAGTG GATCCACTAG TTCTAGAGCG GCCGCATGGA
              121 GGGATTCGAC GGTTCAGATT TTAGTCCTCC AGCTGATTTA GTTGGCGTTG ACGGTGCAGT
              181 CATGCGGAAC GTCGTTGACG TCACGATAAA TGGTGACGTC ACTGCTCCGC CGAAAGCTGC
              241 GCCAAGAAAA AGTGAATCGG TAAAGAAAGT TCATTGGAAT GATGTAGACC AAGGACCGAG
              301 TGAAAAACCA GAGACAAGAC AGGAGGAACG AATAGATATA CCCGAGATTT CAGGTCTATG
              361 GTGGGGCGAG AATGAACATG GAGTGGACGA TGGGAGAATG GAGATACCTA CTACTGGTGT
              421 AGGTCGCGTC CAGTTTCGTG TCCGAGCAGT GATTGATCAT CTAGGGATGC GAGTCTTTGG
              481 AGTCTTCCTA ATTTTCTTGG ACATCATCCT CATGATCATT GATCTCAGTC TTCCAGGAAA
              541 AAGTGAATCT TCACAATCCT TTTATGACGG GATGGCTTTG GCTCTTTCTT GTTATTTCAT
              601 GCTGGATTTA GGATTAAGGA TATTTGCCTA CGGGCCCAAG AATTTCTTCA CCAACCCCTG
              661 GGAGGTTGCT GATGGTTTGA TTATCGTTGT CACATTCGTC GTCACGATAT TTTACACTGT
              721 GTTAGATGAA TACGTGCAAG AAACAGGAGC CGATGGTTTG GGGCAATTGG TTGTGTTGGC
              781 CCGTTTGCTG CGTGTGGTTC GATTAGCAAG AATATTTTAT TCCCACCAAC AAATGAANGN
              841 TTCAAGCAGA AGAACAATAT CANGGGNNCC CATGANTGTG ATTAAAGANG AAATGAAGAT
              901 CAAGCTGCAT ATGGAAGGAA ATGTNAANNN NCNTGNNTTT GTGATTGNNN NNNANGGNAA
            961 NNNAAAGNNT TANNATGGGN NNCAGANTTT NAANNNGANA NTGNANNNNN NNNNN
            //
            Primer: mKOk-C_F (Pr0645)
            Region: insert 3'(Marmeid 3' SV40 pA,delta Tol2 ITR )
            Sequence file: RDB15780_A8D8g.seq check
            >D04498J8_A8D8_3_mKOk-C_F_H03_24_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NACNTCGGCN NTCGCCTCGT CNGGAAAACC GAAGGCAACA
               61 TTACTGAGCA GGTAGAAGAT GCAGTAGCTC ATTCCTAATC TAGATCATAA TCAGCCATAC
              121 CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA
              181 ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA
              241 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
              301 TGGTTTGTCC AAACTCATCA ATGTATGCGG CCGCACTAGT CTCGAGTCTA GAGATCTGCG
              361 AAGATACGGC CACGGGTGCT CTTGATCCTG TGGCTGATTT TGGACTGTGC TGCTCGCAGC
              421 TGCTGATGAA TCACATACTT CCTCCATTTT CTTCCACTGA TTGACTGTTA TAATTTCCCT
              481 AATTTCCAGG TCAAGGTGCT GTGCATTGTG GTAATAGATG TGACATGACG TCACTTCCAA
              541 AGGACCAATG AACATGTCTG ACCAATTTCA TATAATGTGA AAACGATTTT CATAGGCAGA
              601 ATAAATAACA TTTAAATTAA ACTGGGCATC AGCGCAATTC AATTGGTTTG GTAATAGCAA
              661 GGGAAAATAG AATGAAGTGA TCTCCAAAAA ATAAGTACTT TTTGACTGTA AATAAAATTG
              721 TAAGGAGTAA AAAGTACTTT TTTTTCTAAA AAAATGTAAT TAAGTAAAAG TAAAAGTATT
              781 GATTTTTAAT TGTACTCNAG TAAAGTAAAA ATCNNNAAAA ATAATACTTA AGTACAGTAA
              841 TCAAGTAAAA TTACTCNAGT ACTTTACACC TCTGGNTCTN GANCCCCTAC CTTCAGCNAG
              901 CCCAGCANAT NCNCTANGGC CGCCNCCGCN GNGGANCTCC AGCTTTTGNN CCCTTTANTG
              961 AGGGNTAATT GNNCNCTNGG CGNNNNNNTG NNCNTANCTN NTTNNNGNGT GAAATTGNNA
            1021 TNNNNNNNNN ATNNNNNNNN NN
            //
            Primer: mKO2-N_R (Pr0494)
            Region: insert mid(Mermaid mid)
            Sequence file: RDB15780_A8D8h.seq check
            >D04498J8_A8D8_3_mK02-N_R_B03_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTGCN NNTNGNNGGN NCCGTCNNTG TAGTACCTCA TCTTCNTCTC
               61 TGGTTTAATC ACACTCACCA TGGTCTCGAG CTTCGAAGCC TGACTTGGCA GCAAAGAATT
              121 GCGAGCAACC GCATTCTCAT ACAGCTTGAC TTTGCTGTAA TCCCTGTCAT GGCTCAAGAT
              181 CTCGATACGA TGGTCGATCT TGTGGGCATC TGGCAACTGC ACAACCTTTT TCGCTTTGTA
              241 GGAAGTTTTG AAGTCACATC GGTGATGGCC ACCTCCTTCA AGCAACAGAG CCATGTTAAC
              301 ATCACCCATC AGAAACCCAT CACGCACGTA CATCATCTCA GTGGATGGTT CCCACTTCAA
              361 AGTTTTCTTC TGCATAACTG GACCATTGGG GGGAAAGTTC TTGCCATCAA ACCGAATTTT
              421 ATAGATAAAG CAGTCGCCTT CCATGCTGAT CTCGCTTCTC ACGTTGCAAA TGGCGTTGTC
              481 TTCATAACTC ATGGTTCTTT CCCATGAATA CCCCTCGGGA AACGTCTGCT TGAAATAGTC
              541 TGGTATATCG GCTGGATATT TAGTGAATGC TCTATTTCCG TACTGGAACG CATTTGTCAA
              601 GATGTCGTAA GAAAAAGGGA GAGGTGCGCC TTCTTTCACT GTCAGGTTTA AAGTCTGTGT
              661 CCCATCGTAA GGCTTTCCTT TTCCATCTCC TTCAATCACA AATGCATGAN CGTTTACATT
              721 TCCTTCCATA TGCAGCTTGA TCTTCATTTC CTCTTTAATC ACACTCATGG GATCCCCTGA
              781 NATTGNTCTT CTGCTTGAAG CCNTCATTTG NTGGNGGGAA TAAAATANTC TTGCTNNNCN
              841 AANNNCACNN ANCAAANGGG NCNACACNNC NNNNNNNCCC NAANNNTNNN NTNNNGNTTN
            901 NNGNNNGTNN NNNNNNNN
            //
            Primer: SV40polyA_R2 (Pr0623)
            Region: insert mid(Mermaid 3')
            Sequence file: RDB15780_A8D8i.seq check
            >D04498J8_A8D8_3_SV40polyA-R2_B02_05_ABI24.ab1
                1 NNNNNNNNNN NNNCNNNNAA NNNGTTAACA ACAANNATTG CATTCNTTTT ATGTTTCAGG
               61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
              121 CTGATTATGA TCTAGATTAG GAATGAGCTA CTGCATCTTC TACCTGCTCA GTAATGTTGC
              181 CTTCGGTTTT CCTGACGAGG CGATGGCCGA TGTAATGGTC TCCTGGCATT TCAAGAATCT
              241 CTTTTGCCGC CTTGTAAGTA GTCTTGAATT GGCATTTGTG ATTGCCGCCT CCTTCAAGTT
              301 TTAGGTACAT CGTAACATCA CCCTTCAGAA CTCCGTCGCT GGCAGTAATT TTCTCGGTTG
              361 ATGGCTCCCA ATCAACACTT TGGTTTTGCA TGATAGGACC ATCGGCAGGA AAGTTAACCC
              421 CAGTAAATTT GGATTTGTGG TAGAAGGTGT TTCCTCTAAG GCTTATATGC GCACTGACTG
              481 AAGCGGACCC ACCATCTTCG AACTCCAACG ACCTTTCCCA TGACAGGCCT TCAGGAAATG
              541 CTTGTTTGAA ATAGTCTGGT ATCTCTTCTG GATATTTAGT AAATACTCTG TGGCCGTAAC
              601 AGAACACGTG TGACACTAAG TCAAACGCGA AAGGCATTGG CCCGCCCTCG GCCATTGTGA
              661 CGCGTAGTGT CATCTCTTGA TGTCCCTCGT AAGGTCTGCC TGTGCCTTCA CCTTCNATTG
              721 TGAACTCATG CCCATTGACG GANCCGTCCA TGTAGTACCT CATCTTCNTC TCTGGTTTAA
              781 TCACACTCAC CATGGTCTCG AGCTTCGAAG CCTGANTTGN CAGCAAAGAA TTGCGAGCAA
              841 CCGCATTCTC ATACAGCTTG ANTTTGCTGT AATCCCTGTC ATGNNTCAAG ATCTCGATAN
              901 NATGGTCGAT CTTGTGGNNA TCTNGNNACN NNNCNACCTT TTNNNCTTTG NNNGNNNTTT
              961 TGAANTCNCN TCNGNNANNG NCNNNNNNNT NNNNNCNNNN NANCNNTGNN NNCNTCACCN
            1021 NNNNNAANNN NNNNNNN
            //
            Primer: cmlc2pro_R (Pr0644)
            Region: insert 5'(cmlc2 pro),Tol2 1
            Sequence file: RDB15780_A8D8j.seq check
            >D04498J8_A8D8_3_cmlc2pro_R_G03_21_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNCN NTGCCTGATT TTTATNNCTA TTGAATCAAT CATTTTAAAG
               61 AATGTTAAGT CTGCTGATTT GGAGAGCTCA TTTACATTTA TTTTGAATGT CTCTTATTTA
              121 ACACAACTGA TTTAAGCTTA TCGATACCGT CGACCTCGAG GGGGGGCCCA TCTGGCCTGT
              181 GTTTCAGACA CCAGGGAGTC TCTGCTCACG TTTCCTGCTA TTTGCAGCCT CTCTATCAAG
              241 ACTAATACAC CTCTTCCCGC ATCGGCTGCC TGTGAGAGGC TTTTCAGCAC TGCAGGATTG
              301 CTTTTCAGCC CCAAAAGAGC TAGGCTTGAC ACTAACAATT TTGAGAATCA GCTTCTACTG
              361 AAGTTAAATC TGAGGTTTTA CAACTTTGAG TAGCGTGTAC TGGCATTAGA TTGTCTGTCT
              421 TATAGTTTGA TAATTAAATA CAAACAGTTC TAAAGCAGGA TAAAACCTTG TATGCATTTC
              481 ATTTAATGTT TTTTGAGATT AAAAGCTTAA ACAAGAATCT CTAGTTTTCT TTCTTGCTTT
              541 TACTTTTACT TCCTTAATAC TCAAGTACAA TTTTAATGGA GTACTTTTTT ACTTTTACTC
              601 AAGTAAGATT CTAGCCAGAT ACTTTTACTT TTAATTGAGT AAAATTTTCC CTAAGTACTT
              661 GTACTTTCAC TTGAGTAAAA TTTTTGAGTA CTTTTTACAC CTCTGTCAAG AACTCCTGGA
              721 CAAACCTCTG ACCTGTGTGG AACAGAGTGG ATATGGGTGT CTGAACAGAT ATTCACGTCT
              781 TTTGCAGATC AGANGGCATT TCTGGTGTTG TGCAATGACC TGGGTCCNAC ANGTAGGCGT
              841 ACTCGTATCC NAGAGCATTG TTGGANAGGA NGTAGTCTCC ATTCCTATAA AGAGGAANNA
              901 ATGGNACCNT GTANTANCCG TCATTGTGGN CAATTNNTGC NNTGGNACGT GGGTAGATGG
              961 NNNGGGGNNG NTGANGANTT CTNANCNNGC NCTCNAANNN GCTANAAATG ANGTNNNNNN
            1021 NNNN
            //
            Primer: RDB15263#2 (Pr0525)
            Region: insert mid(Mermaid mid)
            Sequence file: RDB15780_A8D8k.seq check
            >15780_15780_B3CmF_RDB15263#2_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNA TCCTCNTGNT CATTGATCTC AGTCTTCCNG GAAAAAGTGA
               61 ATCTTCACAA TCCTTTTATG ACGGGNTGGC TTTGGCTCTT TCTTGTTATT TCATGCTGGA
              121 TTTAGGATTA AGGATATTTG CCTACGGGCC CAAGAATTTC TTCACCAACC CCTGGGAGGT
              181 TGCTGATGGT TTGATTATCG TTGTCACATT CGTCGTCACG ATATTTTACA CTGTGTTAGA
              241 TGAATACGTG CAAGAAACAG GAGCCGATGG TTTGGGGCAA TTGGTTGTGT TGGCCCGTTT
              301 GCTGCGTGTG GTTCGATTAG CAAGAATATT TTATTCCCAC CAACAAATGA AGGCTTCAAG
              361 CAGAAGAACA ATATCAGGGG ATCCCATGAG TGTGATTAAA GAGGAAATGA AGATCAAGCT
              421 GCATATGGAA GGAAATGTAA ACGGTCATGC ATTTGTGATT GAAGGAGATG GAAAAGGAAA
              481 GCCTTACGAT GGGACACAGA CTTTAAACCT GACAGTGAAA GAAGGCGCAC CTCTCCCTTT
              541 TTCTTACGAC ATCTTGACAA ATGCGTTCCA GTACGGAAAT AGAGCATTCA CTAAATATCC
              601 AGCCGATATA CCAGACTATT TCAAGCAGAC GTTTCCCGAN GGGTATTCAT GGGAAAGAAN
              661 CATGANTTAT GAANNNNACG NCNTTTGCAA CGTGANANNC NANNNCNNNA NNNNANNNNN
            721 NNNNN
            //
            Primer: mUGK-B_F (Pr1311)
            Region: insert mid(Mermaid mid)
            Sequence file: RDB15780_A8D8l.seq check
            >15780_15780_B3CmF_mUGK-B_F_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNGCNNN NNNNAGCTGT ATGAGAATGC GGTTGCTCGC
               61 AATTCTTTGC TGCCAAGTCA GGCTTCGAAG CTCGAGACCA TGGTGAGTGT GATTAAACCA
              121 GAGATGAAGA TGAGGTACTA CATGGACGGC TCCGTCAATG GGCATGAGTT CACAATTGAA
              181 GGTGAAGGCA CAGGCAGACC TTACGAGGGA CATCAAGAGA TGACACTACG CGTCACAATG
              241 GCCGAGGGCG GGCCAATGCC TTTCGCGTTT GACTTAGTGT CACACGTGTT CTGTTACGGC
              301 CACAGAGTAT TTACTAAATA TCCAGAAGAG ATACCAGACT ATTTCAAACA AGCATTTCCT
              361 GAAGGCCTGT CATGGGAAAG GTCGTTGGAG TTCGAAGATG GTGGGTCCGC TTCAGTCAGT
              421 GCGCATATAA GCCTTAGAGG AAACACCTTC TACCACAAAT CCAAATTTAC TGGGGTTAAC
              481 TTTCCTGCCG ATGGTCCTAT CATGCAAAAC CAAAGTGTTG ATTGGGAGCC ATNNNNNNNA
              541 GAAAATTACT GCCAGCGACG GAGTTCTGAA GGGTGATGTT ACNATGTANN NNNNACTTGA
              601 ANNNNNCGGC AATCACAAAT GCNANNTCAN GANTACTTAC NNNNNGGCAA AAGAGANTCN
              661 TGAAANNNNC ANNNAGANNN NNNACATCNN NNNNCNCCNC GTCANGNAAA ACCNNANNGN
            721 NAACNTTNNN NNNANCNNGN NNNANNNNNN NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Tsutsui, H., Visualizing voltage dynamics in zebrafish heart. J. Physiol. 588 (Pt 12): 2017-2021 (2010). PMID 20421282. [link to RRC of NBRP]

            2024.03.26

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