Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pUC-csm5h

Plasmid for introduction of (His)6-tag-coding sequence at the 3'-terminal of the csm5 gene (TTHB151) with kanamycin resistance marker.

Catalog number RDB15776
Resource name pUC-csm5h
Clone info. Plasmid for introduction of (His)6-tag-coding sequence at the 3'-terminal of the csm5 gene (TTHB151) with kanamycin resistance marker gene by means of homologus recombinationon the genome of Thermus thermophilus HB8.
Comment Host strains Stbl2 and Stbl3 are recommended. Expression was confirmed by the depositor with purification of Csm complex using Ni-resin colum.
Vector backbone pUC19 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r (E. coli)
Gene/insert name Thermus thermophilus HB8 TTHB151 genomic DNA Staphylococcus aureus kanamycin nucleotidyltransferase (HTK) genomic DNA Thermus thermophilus HB8 TTHB152 genomic DNA
Depositor|Developer Shinkai, Akeo |
Other clones in our bank

External Database
Thermus thermophilus HB8 TTHB151 Staphylococcus aureus kanamycin nucleotidyltransferase (HTK) Thermus thermophilus HB8 TTHB152

      Reference sequence
        

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Staals RHJ, et al. Mol. Cell 56, 518-530, 2014)
      Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
      Order form 
      [Credit Card Payment]  [Bank Transfer Payment]
      Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
      提供案内 (日本国内) [open/close]

      提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Staals RHJ, et al. Mol. Cell 56, 518-530, 2014)。
      提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
      提供依頼書 [Word]
      提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

      Catalog # Resource name Availability Shipping form Fee (non-profit org.)
      RDB15776 pUC-csm5h Under QC test. Please contact us. DNA solution

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

      References and tips

      Electronic file

      Original reference

      original Staals, R.H., RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus. Mol. Cell 56 (4): 518-530 (2014). PMID 25457165.

      Further references such as user reports and related articles (go to bottom)

      Featured content


      Sequence information

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


      References

      Original, user report and related articles

      original Staals, R.H., RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus. Mol. Cell 56 (4): 518-530 (2014). PMID 25457165.

      2022.05.17

      GNP_filter3_RDBDEP_html_220516.pl