RDB15765 - pHSP70-EGFP

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pHSP70-EGFP

Heat-shock-inducible EGFP reporter construct.

Catalog number	RDB15765
Resource name	pHSP70-EGFP
Clone info.	Heat-shock-inducible reporter construct. EGFP was placed under the control of human HSP70b' (heat shock protein family A member 6 (HSPA6)) promoter corresponding -456 to -6-bp region (relative to translation start) of human HSPA6 (NCBI Accession No. DQ521571.1). See Fig. 3 of Nakatsuji, H. et al., Sci. Rep., 7 (1): 4694, 2017.
Comment	Expression was confirmed by the depositor with fluorescence assay. Known differences: A to C substitution at 2222nt of DQ521571.1.
Vector backbone	pEGFP-N1 (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	Human HSPA6 genomic DNA
Depositor\|Developer	Kengaku, Mineko \|

Other clones in our bank

human HSPA6

External Database
Human HSPA6

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuji, H. et al. Sci. Rep. 7(1): 4694, 2017)

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuji, H. et al. Sci. Rep. 7(1): 4694, 2017)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nakatsuji, H. et al. Sci. Rep. 7(1): 4694, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15765	pHSP70-EGFP	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Original reference

original	Nakatsuji, H., Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods. Sci. Rep. 7 (1): 4694 (2017). PMID 28680130.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15765_A8Ajp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N () Sequence file: RDB15765_A8Aja.seq
>D05107A1_A8Aj_1_EGFP-N_A08_01_ABI08.ab1 1 GTACCCGGGG CTTACGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCCGGG 61 CCCGCGGTAC CGTCGACTGC AGAATTCGAA GCTTCTTGTC GGATGCTGGA GGCCACGGAG 121 GCTGCTGCCG CTCCGCCGTC GAGGGGCGCT CAGCAGTGAG GCTCTCCCTG CGGTTTCTCT 181 GCAGCCAGCC CGGCTCGGAT CTGCTCAGCG CCGCTTCCAC GGGCTTTTTA TCCTTTTGCG 241 CCTCACCCGA CCCCCGCCGC CGCGAACTTT CGCGAACCTT TCCGCACCTT CCCGCACCTT 301 CCCGCCCAGT TGAGAGCCTC TCGCCCGCCC GGGCTGACTC AGCCGGGTGG CGCGGCGCGG 361 CGCTGACCCC GGGAGAAGGT TCGGGCAGAT CGGGGCATGA GCCGCGAGGC TGGGCGGCTA 421 CGCCAGGCCA GCCCGCCTCT GACTCAGGCT GCTGAAAGAA ATGCGGGGAA GGTTCTAGCT 481 CCTCCGGGCT GGCTCTGAGG ACGCGCGGTT GTGGCAGAGG CTGCAAAAGC AGCAACTTTA 541 GAGGATTAAT AACTAATGCA TGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 601 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 661 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 721 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 781 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 841 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 901 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA AACCCCCCCG TTCAGCCCGA CCGCTGCGCC 961 TTATCCGGGT AACTATCGTC TTTGAGTCCC AACCCCGGGT AAGGACACGA CTTTATCGCC 1021 ACTGGGCAGC AAGCCAACTT GGTAACAGGA TTTAGCAGAG CGAAGGTTAT GTAAGCGGTG 1081 CTAACAGAGT TCTTTGAAGG TGTGGCTTAA CTTACGGCTA CACTAGGAGA GCCAGGTATA 1141 TTTTGGGGT //
Primer: pAxEF R2 () Sequence file: RDB15765_A8Ajb.seq
>D05107A1_A8Aj_1_pAxEF_R2_B08_02_ABI08.ab1 1 CGTGGGTAAA ATGCTTTATT TGTGAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT 61 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG 121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA 181 TGATCTAGAG TCGCGGCCGC TTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC 241 GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG 301 GGCGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG 361 GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT 421 CTTGAAGTTC ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT 481 GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC 541 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT 601 GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC 661 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC 721 GTAGGTCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT 781 GAACTTCAGG GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA 841 CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG 901 CTCCTCGCCC TTGCTCACCA TGGTGGCGAC CGGTGGATCC CGGGCCCGCG GTACCGTCGA 961 CTGCAGAATT CGAAGCTTCT TGTCGGATGC TGGAGGCCAC GGAAGGCTGC TGCCGCTCCG 1021 GCCGTCGAGG GGCGGCTTCA GCAGTGAGGC TCCTCCCTGC GGGATTCTTC TGCAGCCAGC 1081 CCTGGCCTCG GAATCTGCTT CAGCGGTCGC ATCCACGGAC TTTTTTATCC TTTTTTGGCG 1141 TCCTTCATCC TCGA //
Primer: SV40pro_F () Sequence file: RDB15765_A8Ajc.seq
>D05107A1_A8Aj_1_SV40pro_F_C08_03_ABI08.ab1 1 GGTTCGGGGG TTAGCCTTTC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG 61 CAAAGATCGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC 121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA 181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT 241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA AGACGAGGCA GCGCGGCTAT 301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG 361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG 421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC 481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA 541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG 601 CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC 661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG 721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA 781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG 841 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC 901 TCTGGGGTTC GAAATGACCG ACCCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 961 CCACCGCCGC CTTCTATGAA AGGGTTGGGG CTTCTGAATC GTTTTCCGGG ACGCCCGGCT 1021 GGGATGATCC CTCCAGCGCG GGGGATCTCA TGCTGGGAGG TTCTTTCGCC CCACCCTACG 1081 GGGAGCTAAC TGAACACGAG AGACCATTAC TGGAAGGAAC CCGCCGCCTA GTGACGGGCC 1141 TAAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nakatsuji, H., Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods. Sci. Rep. 7 (1): 4694 (2017). PMID 28680130.

2023.04.06

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