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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-mouse Rab32(Q83L)

Expression vector of mouse Rab32 constitutive active mutant EGFP-tag.

Catalog number RDB15734
Resource name pEGFP-C1-mouse Rab32(Q83L)
Clone info. Expression vector of mouse Rab32 constitutive active mutant (GTP-hydrolysis-deficient, Q83L), fused with EGFP at N-terminal. CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope. Please refer Itoh, T. et al., Genes. Cells, 11 (9): 1023-1037, 2006 (PMID 16923123) for gene cloning.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab32 cDNA
Reference of insert sequence AB232614 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab32

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012)。
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          RDB15734 pEGFP-C1-mouse Rab32(Q83L) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEGFP-C1-mouse Rab32(Q83L) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15734).

          Reference section:

          Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          Featured content

          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15734_A8A9p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP 3',insert
          Sequence file: RDB15734_A8A9a.seq check
          >D05154B2_A8A9_1_EGFP-C2_C08_08_ABI24.ab1
              1 NNNNNNNNNN NCNCGNNCNC NTGGNCCTGC TGGNNTTCGT GNNCGCCGCC GGGATCACTC
             61 TCGGCATGGA CGAGCTGTAC AAGTCCGGAC TCAGATCCAT GGCGGGCGAG GGACTAGGGC
            121 AACAGGGCGC GTCGGCCACC GCGGCGCCGG AGACCCGAGA GCACCTCTTT AAGGTGCTGG
            181 TCATCGGCGA GCTCGGCGTG GGTAAGACGA GCATCATCAA GCGCTACGTG CACCAGCTCT
            241 TCTCCCAGCA CTACCGCGCC ACCATTGGCG TGGACTTCGC CCTCAAAGTT CTCAACTGGG
            301 ACAGCAGGAC GCTCGTGCGC CTGCAGCTGT GGGATATCGC GGGACTGGAA CGGTTTGGCA
            361 ACATGACTCG AGTATACTAT AAGGAAGCTC TTGGAGCATT TGTAGTCTTT GATATATCGA
            421 GAAGTTCTAC ATTTGATGCA GTCCTAAAAT GGAAAAACGA TCTGGATAGT AAAGTTCATC
            481 TTCCCAACGG CAGCCCCATT CCTGCTGTCC TCCTAGCTAA CAAATGTGAC CAGAAGAAGG
            541 ACAACAGCCA GAGTCCTTCC CAGATGGACC AGTTTTGTAA AGACCATGGC TTCACTGGAT
            601 GGTTTGAAAC CTCTGCCAAG GATAATATAA ACATCGACGA GGCCACCCGC TTCCTAGTGG
            661 AAAACATGCT TGCAAACCAG CAAAGTTTTC CTAGTGAAGA AATCGACCTG GACAGAATTA
            721 AACTGGTAGA GGAGCCCCCC ACAACAAAGC CCAGGTCCCA GTGCTGCTGA TGTGTCTCTG
            781 TGAATCGAAT TCGATATCAA GCTTATCGAT ACCGTCGANN GTACCGCGGG CCCGGGNTCC
            841 ACCGGNNCTA GATAACTGAT CATAATCAGC CNTACCACAT TNGTAGAGGN TTNACTTGCT
            901 TTNAAAAAAC NTNCNNCANC NCCCCCCNGN ANNNTNAANN NTAAAANGAA TGNNNNNGNN
          961 NNNGNNNNNN NNNTNNNNNN ANCNTNANNN NNNNTNNNNA NNNANNNNNN NNNN
          //
          Primer: SV40polyA_R2 (Pr0623)
          Region: insert 3'
          Sequence file: RDB15734_A8A9b.seq check
          >D05154B2_A8A9_1_SV40polyA-R2_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGTTNNCAA CNACAATTGC ATTCNTTTTA TGTTTCNGGT
             61 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
            121 TGATTATGAT CAGTTATCTA GATCCGGTGG ATCCCGGGCC CGCGGTACCG TCGACGGTAT
            181 CGATAAGCTT GATATCGAAT TCGATTCACA GAGACACATC AGCAGCACTG GGACCTGGGC
            241 TTTGTTGTGG GGGGCTCCTC TACCAGTTTA ATTCTGTCCA GGTCGATTTC TTCACTAGGA
            301 AAACTTTGCT GGTTTGCAAG CATGTTTTCC ACTAGGAAGC GGGTGGCCTC GTCGATGTTT
            361 ATATTATCCT TGGCAGAGGT TTCAAACCAT CCAGTGAAGC CATGGTCTTT ACAAAACTGG
            421 TCCATCTGGG AAGGACTCTG GCTGTTGTCC TTCTTCTGGT CACATTTGTT AGCTAGGAGG
            481 ACAGCAGGAA TGGGGCTGCC GTTGGGAAGA TGAACTTTAC TATCCAGATC GTTTTTCCAT
            541 TTTAGGACTG CATCAAATGT AGAACTTCTC GATATATCAA AGACTACAAA TGCTCCAAGA
            601 GCTTCCTTAT AGTATACTCG AGTCATGTTG CCAAACCGTT CCAGTCCCGC GATATCCCAC
            661 AGCTGCAGGC GCACGAGCGT CCTGCTGTCC CAGTTGAGAA CTTTGAGGGC GAAGTCCACG
            721 CCAATGGTGG CGCGGTAGTG CTGGGAGAAG AGCTGGTGCA CGTAGCGCTN GATGATGCTC
            781 GTCTTACCCA CGCCGANCTC GCCGATGANC AGCACCTTAA AGANGTGCTC TCGGGTCTCC
            841 NGCGCCGCGG NGGCCGANGC GCCCNGTTGC CCTANTCCCN CGCCCGCCAT GNATCTGANT
            901 CCGGANTNNN ACAGNNCNNN CNNGNNNANN NNGNNNCNNG NNGCNNNCAC GAACNCCNNC
          961 NNNANNNTNN NNNNNNNCNT NNNNNNNGGG NNNNTNNNNN NNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: EGFP 5'
          Sequence file: RDB15734_A8A9c.seq check
          >D05154B2_1_A8A9_CMV-Forward_F05_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGCGGNANGN GNNGNNNNGN GNNNNNNNNN
             61 NNNNNGNNNN NNNGGTTNNN NGNNNNGTCA GATCCGCTNG CGCTACCGGT CGCCACCATG
            121 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
            181 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
            241 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
            301 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG
            361 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
            421 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
            481 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
            541 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC
            601 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAN
            661 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
            721 CTGANCACCC AGTCCGCCCT GANCAAAGAC CCCAACGANA ANCGCGATCA CATGGNCCTG
            781 CTGGANTTCN NGANCGCCGC NNGGNNCANT CTCNGNATGG ANNANCTGTA NNNGTNCGGA
          841 NTCNNATNNN NNGNNGGNNA GGNNNNAGGN NAACNNGNNN NNNNNNN
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR_KanR
          Sequence file: RDB15734_A8A9d.seq check
          >D05154B2_1_A8A9_SV40pro_F_V2_G05_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGCTTTTGN AAGNNCGATC NAGAGANAGG NNGAGGNNCG
             61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
            601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
            721 CANGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGNCGGCGA ATGGGCTGAN
            781 CGCTTCCTCG NGCTTTACGG TATCGCCGCT CCCGATTCGC ANCGCATCGC CTTCTATCGC
            841 CNTCTTGNNN AGTTCNTCTN ANCGGGANTN NNGGGNTCGA AATGANCGAN NNANNNANNN
          901 CNNNCCNNNC NNNNNCNANA TTTCNANTNC NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
          reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]

          2023.11.21

          GNP_filter3_RDBDEP_html_231027.pl