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pMRX-bsr-Myc-mouse Rab20-SR

Retroviral expression vector of siRNA-resistant (SR) form of mouse Rab20.

Catalog number RDB15731
Resource name pMRX-bsr-Myc-mouse Rab20-SR
Clone info. Retroviral expression vector of siRNA-resistant (SR) form of mouse Rab20, tagged with Myc at N-terminal. CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting. Please refer Kuroda T.S. et al., J. Biol. Chem., 277 (42): 9212-9218, 2002 (PMID 12176990) for gene cloning.
Vector backbone pMRX-IRES-bsr (plasmid)
Selectable markers Amp^r (E. coli), Blasticidin^r (mammalian cell).
Gene/insert name mouse Rab20 cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
mouse Rab20
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda T.S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Oguchi et al. Neurosci. Lett., 662, 324-330, 2018)
          Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda T.S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Oguchi et al. Neurosci. Lett., 662, 324-330, 2018)。
          付加的提供条件 pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB15731 pMRX-bsr-Myc-mouse Rab20-SR Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Oguchi, M.E., Rab20, a novel Rab small GTPase that negatively regulates neurite outgrowth of PC12 cells. Neurosci. Lett. 662: 324-330 (2018). PMID 29107708.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Oguchi, M.E., Rab20, a novel Rab small GTPase that negatively regulates neurite outgrowth of PC12 cells. Neurosci. Lett. 662: 324-330 (2018). PMID 29107708.
          reference Fukuda, M., Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J. Biol. Chem., 277, 39673-39678 (2002). PMID 12176990.

          2022.05.17

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