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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSIX3G137C

Plasmid clone of G137C mutant of secreted in xylem3 (SIX3) gene of Fusarium oxysporum f. sp. lycopersici (Fol).

Catalog number RDB15722
Resource name pSIX3G137C
Clone info. Plasmid clone of G137C mutant of secreted in xylem3 (SIX3) gene of Fusarium oxysporum f. sp. lycopersici (Fol). This clone was used in the loop-mediated isothermal amplification (LAMP) assay.
Comment Wild-type and variants mentioned in Ayukawa, Y. et al., Sci. Rep., 7 (1): 4253, 2017 can be acquired from the natural environment. GenBank: AM234063.1
Vector backbone pGEM-T easy (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Fusarium oxysporum f. sp. lycopersici SIX3
Depositor|Developer Komatsu, Ken |
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External Database
Fusarium oxysporum f. sp. lycopersici SIX3
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR For profit-use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from The University Research Administration Center, Tokyo University of Agriculture and Technology ( suishin@ml.tuat.ac.jp ). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature and state an acknowledgement to the DEPOSITOR. (Ayukawa, Y et al. Sci. Rep. 7(1): 4253, 2017)
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          提供条件 営利目的利用の希望については、希望者は予め農工大研究推進センターに連絡し、承諾を得る。利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 (Ayukawa, Y et al. Sci. Rep. 7(1): 4253, 2017)。
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          RDB15722 pSIX3G137C DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ayukawa, Y., Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi. Sci. Rep. 7 (1): 4253 (2017). PMID 28652587.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15722_A8A9p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB15722_A8A9a.seq check
          >D05148A1-1_A8A9_M13_-40_E01_13_ABI24.ab1
              1 GGCAGAGGTG AAATTGTAAT ACGGACTCAC TATAGGGCGA ATTGGGCCCG ACGTCGCATG
             61 CTCCCGGCCG CCATGGCGGC CGCGGGAATT CGATTTATAT TACCGACCAT CTTGCCTAAA
            121 CATTTACCAC TATGCGTTTC CTTCTGCTTA TAGCCATGTC CATGACATGG GTTTGCTCTA
            181 TTGCTGGGCT ACCTGTGGAA GATGCCGATT CATCTGTCGG TCAGCTCCAA GGACGGGGTA
            241 ACCCATATTG CGTGTTTCCC GGCCGCCCCA CGTCTTCTAC TTCATTTACT ACGAGCTTCA
            301 GCACCGAACC TCTGGGTTAT GCTAGAATGT TACACAGAGA CCCTCCATAT GAGCGGGCTG
            361 GCAATTCGGG ACTGAATCAC CGCATTTACG AACGAAGTCG CGTTGGAGGC CTCCGCACTG
            421 TCATTGATGT CGCCCCCCCG GACGGACACC AAGCCATCGC CAACTACGAG ATCGAAGTCC
            481 GTCGTATTCC TGTTGCAACA CCTAATGCGG CTGGAGATTG CTTTCACACT GCACGCCTTA
            541 GTACCGGCTC TCGAGGTCCA GCCACAATCA GTTGGGATGC CGACGCTAGT TATACCTACT
            601 ATCTTACTAT CTCAGAGGAT TGATTCTGTG GCAGTTCCCC TTGGCAATCA CTAGTGAATT
            661 CGCGGCCGCC TGCAGGTCGA CCATATGGGA GAGCTCCCAA CGCGTTGGAT GCATAGCTTG
            721 AGTATTCTAT AGTGTCACCT AAATAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG
            781 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA
            841 GCCTGGGGTG CCTAATGGAG TGGAGCTAAC TCACATTAAA TGCCGTTGCG CCTCCACTGC
            901 CCGCTTTCCA GTCGGGAAAA CTTGTCCGTG CCAGCCTGCA TAATTGAATC GGCACCGCCG
            961 GGAGAAGGCG TGCGATATTG GGGGCCTTTC CTGTTCCTCG TCCATGAATC CTTGCCTCGG
           1021 CTTCCGTGCG CGAAGGTACG ATCACTAGCG GTATCGGTAC CAATCGATAC GAAACTGTGC
          1081 AACGG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Ayukawa, Y., Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi. Sci. Rep. 7 (1): 4253 (2017). PMID 28652587.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl