RDB15713 - pStMuPEP1(WT)

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pStMuPEP1(WT)

Expression vector of Streptococcus mutans peptidase domain 1 (PEP1) of ComA, wild type.

Catalog number	RDB15713
Resource name	pStMuPEP1(WT)
Clone info.	Expression vector of Streptococcus mutans peptidase domain 1 (PEP1) of ComA, wild type. The hydrophobic N-terminal regions (48 amino acids, comA) has been truncated from the PEP, tagged with His at C-terminus.
Comment	PEP of ComA PEP of S. mutans (strain ATCC No. 700610) was generated by PCR from pSMuP1. The primers used were 5'-ACATATGTATAAGCTAGTACCTCAGATTGATAC-3' (forward) and 5'-AGCTTATACATATGTATATCTCCTTCTTAAAGT-3' (reverse). Reference of genome sequence, GenBank AE014133.
Vector backbone	pET21-b (plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r
Gene/insert name	Streptococcus mutans comA genomic
Depositor\|Developer	Ishii, Seiji \|

Other clones in our bank

Streptococcus mutans comA and ortholog

External Database
Streptococcus mutans comA

KEGG Orthology

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, S et al. Sci. Rep. 7(1): 4029, 2017)

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, S et al. Sci. Rep. 7(1): 4029, 2017)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, S et al. Sci. Rep. 7(1): 4029, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15713	pStMuPEP1(WT)	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Ishii, S., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15713_A7Lcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_R2 Sequence file: RDB15713_A7Lca.seq
>D05129A1_A7Lc_1_pBR_BamHI_R2_A11_02_ABI24.ab1 1 CTTGCTGCCA GAGTTTCGAG CGTAGCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC 61 TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC 121 CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGTATA AGCTAGTACC 181 TCAGATTGAT ACCAGAGACT GTGGGCCGGC AGTGCTGGCA TCTGTTGCAA AGCATTACGG 241 ATCTAATTAC TCTATCGCTT ATCTGCGGGA ACTCTCAAAG ACTAACAAGC AGGGAACAAC 301 AGCTCTTGGC ATTGTTGAAG CTGCTAAAAA GTTAGGCTTT GAAACACGCT CTATCAAGGC 361 GGATATGACG CTTTTTGATT ATAATGATTT GACCTATCCT TTTATCGTCC ATGTGATTAA 421 AGGAAAACGT CTGCAGCATT ATTATGTCGT CTATGGCAGC CAGAATAATC AGCTGATTAT 481 TGGAGATCCT GATCCTTCAG TTAAGGTGAC TAGGATGAGT AAGGAACGCT TTCAATCAGA 541 GTGGACAGGC CTTGCAATTT TCCTAGCTCC TCAGCCTAAC TATAAGCCTC ATAAAGGTGA 601 ACACCACCAC CACCACCACT AGGTCGACAA GCTTGCGGCC GCACTCGAGC ACCACCACCA 661 CCACCACTGA GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC 721 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT 781 GCTGAAAGGA GGAACTATAT CCGGATTGGC GAATGGGACG CGCCCTGTAG CGGCGCATTA 841 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 901 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 961 GCTCTAAATC GGGGGGCTCC CTTTAGGGTT CCGGATTTAG TGCTTTACGG CACCTCGACC 1021 CCAAAAACTT TGATTAGGGT GATGGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGATT 1081 TTTCGCCCTT TGACGTTGAA GTTCACGATT CTTTAATAGT GACTTCTTGT TCCAAACCTG 1141 GAAACCAAAC AGCT //
Primer: lacpro_F Sequence file: RDB15713_A7Lcb.seq
>D05129A1_A7Lc_1_laclpro_F_C11_08_ABI24.ab1 1 GATATTAGGT ACATTATTTC GGATGCGCCG GAGAGAGTCA ATTCAGGGTG GTGAATGTGA 61 AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG ACCGTTTCCC 121 GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG GAAGCGGCGA 181 TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC AAACAGTCGT 241 TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG 301 CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG GTAGAACGAA 361 GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC GTCAGTGGGC 421 TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT GCCTGCACTA 481 ATGTTCCGGC GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT ATTATTTTCT 541 CCCATGAAGA CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT CACCAGCAAA 601 TCGCGCTGTT AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG GCTGGCTGGC 661 ATAAATATCT CACTCGCAAT CAAATTCAGC CGATAGCGGA ACGGGAAGGC GACTGGAGTG 721 CCATGTCCGG TTTTCAACAA ACCATGCAAA TGCTGAATGA GGGCATCGTT CCCACTGCGA 781 TGCTGGTTGC CAACGATCAG ATGGCGCTGG GCGCAATGCG CGCCATTACC GAGTCCGGGC 841 TGCGCGTTGG TGCGGATATC TCGGTAGTGG GATACGACGA TACCGAAGAC AGCTCATGTT 901 ATATCCCGCC GTTAACCACC ATCAAACAGG ATTTTCGCCT GCTGGGGCAA ACCAGCGTGG 961 ACCGCTTGCT GCAACTCTCT CAGGGCCCAG GCGGTGAAAG GGCAATCAGC TGTTGCCCGT 1021 CTCACTGGTG AAAAGAAAAA ACCACCCTGG CGCCCAATAC GCAAACCCGC TCTCCCCGCG 1081 CGTTGGCCCG ATTTCATATG CCAGCCTGGG CCACGACAGG TTCCCGACTG GAAAAGCCGG 1141 GGCCAATGGG AGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Ishii, S., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl