Please review the QC test results indicated by icon below as well as clone information before placing your order.

tFucci(CA)2.2/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mTurquoise-hGem(1/110). CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose)[Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16942_A9Ctp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_R (Pr0517) Region: insert 5'(tFucci(CA)2.2 5'),CMV pro.2 Sequence file: RDB16942_A9Cth.seq
>15454_16942_A9Ct_mCherry-C_R_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNTNNNN GTTGNNGTTG TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG    61 GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC   121 TGCTTGATCT CGCCCTTCAG GGCGCCGTCC TCGGGGTACA TCCGCTCGGA GGAGGCCTCC   181 CAGCCCATGG TCTTCTTCTG CATTACGGGG CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC   241 TTCACCTTGT AGATGAACTC GCCGTCCTGC AGGGAGGAGT CCTGGGTCAC GGTCACCACG   301 CCGCCGTCCT CGAAGTTCAT CACGCGCTCC CACTTGAAGC CCTCGGGGAA GGACAGCTTC   361 AAGTAGTCGG GGATGTCGGC GGGGTGCTTC ACGTAGGCCT TGGAGCCGTA CATGAACTGA   421 GGGGACAGGA TGTCCCAGGC GAAGGGCAGG GGGCCACCCT TGGTCACCTT CAGCTTGGCG   481 GTCTGGGTGC CCTCGTAGGG GCGGCCCTCG CCCTCGCCCT CGATCTCGAA CTCGTGGCCG   541 TTCACGGAGC CCTCCATGTG CACCTTGAAG CGCATGAACT CCTTGATGAT GGCCATGTTA   601 TCCTCCTCGC CCTTGCTCAC CATGGTGGAT CCTATGCTAG CGGATCTGAC GGTTCACTAA   661 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG   721 GGCGGANTTG TTACGACATT TTGGAAAGTC NCGTTGATTT TGGTGCCAAA ACAAACTNNN   781 ATTGACGTCA ATGGGGTGGA GACTTGGNNA TCCCCGTGAN TCAAACCGCT ATCCACGCCC   841 ATTGATGTAC TGCCNAAANC GCATCACCAN NGNAATANCG ATGANTNNTA CNNAGATGNA   901 CTGCCNANNN NNAAANTNCN NTNANNNTCN NGTACTGGNN NTNNNGNNNN GNNGNNNNTT 961 TNNCNNNNNN NNNN //
Primer: PRE_rev (Pr0250) Region: WPRE,insert 3'(tFucci(CA)2.2 3') Sequence file: RDB16942_A9Cti.seq
>15454_16942_A9Ct_PRE_rev_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNNN NNGANCNNCN TAGTTAAGAA NACCAGTCAA TCTTTCACAA    61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT   121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT   181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT   241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC   301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG   361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT   421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC   481 CCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA   541 CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGCTT GGACTGGGTG CTCAGGTAGT   601 GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG   661 CGAGCTGCAC GCCGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTGGCC TTGATGCCGT   721 TCTTCTGCTT GTCGGCGGTG ATATAGACGT TGTCGCTGAT GTAGTTGTAC TCCAGCTTGT   781 GCNNCANGAT GTTGCCGTCC TCCTTNNAGT CGATGCCCTT CAGCTCNATG CGGNTCACCA   841 NNNNGTCGNC NTCGANCTTC ACNTCNGCGC GGGNCNNGTA GTTGCCGNCN NCCTNGAANA   901 ANANGGTACG CTNCTGGNNN NNNCNNTCNG GNNTGGCNGA NNTNAANNNN NCNNGCTGCT 961 NCNNNNNNNN NGGNNNNCNG NNNNNNNNNN //
Primer: mCherry-C_F (Pr0453) Region: insert mid(tFucci(CA)2.2) Sequence file: RDB16942_A9Ctj.seq
>15454_16942_A9Ct_mCherry-C_F_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCN GCNNGGCGCC TACNACGTCA ACNTCAAGTT GGANATCACC    61 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC   121 ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC GCTCGAGATG   181 GAGCAGCGCC GCGTCACCGA CTTCTTCGCG CGCCGCCGCC CCGGGCCCCC CCGCATCGCG   241 CCGCCCAAGC TGGCCTGCCG CACCCCCAGC CCCGCCAGGC CCGCACTCCG CGCCCCGGCC   301 TCCGCTACCA GTGGCAGCCG CAAGCGCGCC CGCCCGCCCG CCGCCCCCGG ACGCGACCAG   361 GCCAGGCCAC CGGCCCGCGC GGCCGCGCGG CTGTCGGTGG ACGAGGTTTC CAGCCCCAGT   421 ACCCCCGAGG CCCCAGACAT CCCAGCCTGC CCTTCTCCGG GCCAGAAGAT AAAGAAAACC   481 GGTCGCGCCA AGCGCGGCAG CGGCGCCACC AACTTCAGCC TGCTGAAGCA GGCCGGCGAC   541 GTGGAGGAGA ACCCCGGCCC CCGCGAATTC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC   601 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG   661 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC   721 ACCGGCAAGC TGCCCGTGCC CTGNCCCACC CTCGTGACCA CCCTGAGCTG GNNCGTGCAG   781 TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCANGCCC   841 GANNGNTACG TNCAGGANCG NACCATCTTC TTNNANNNAC GANNGNNNNN ACNANANCNN   901 NNNCNNANNT GNNNTTCGAG GNNNANNCCN NNNNGNANNN NNNTCNNNCT NNNNNNNATC 961 GANTTNNNNN NNNNN //
Primer: Amp_R (Pr0008) Region: AmpR,CMV en.1 Sequence file: RDB16942_A9Ctk.seq
>15454_16942_B2JdF_Amp_R_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT NNNANCNTTT ATNNGGGTTA NTGTCTCATG AGCGGANACA    61 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG   121 TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTGCA CTCTCAGTAC   181 AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT   241 CGCTGAGTAG TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG   301 CATGAAGAAT CTGCTTAGGG TTAGGCGTTT TGCGCTGCTT CGCGATGTAC GGGCCAGATA   361 TACGCGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT   421 TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG   481 ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC   541 AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC   601 AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG   661 GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT   721 CTACGTATTA NTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG   781 TGGATAGCGG TTTGACTCNC GGGGANTTCC NNGTCTCNNN NNNATTGACG TCAATGGNAG   841 TTTGNTTTGN CACCAAAATC NNNGGGACTT TCCAAANTGT CNNNNCANCT CCNNCCCNNT   901 TGACNCAAAT GGGCNGNNNN NNNGTNNNGN GGGANGNCTA TATAAGCANC NCGTTTTGCC 961 NGTNNTGGGT CNCTCNNGNT ANNNCNNNNN NNNNN //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi,5'LTR Sequence file: RDB16942_A9Ctl.seq
>15454_16942_A9Ct_HIVgp_R1_D01_10_ABI24.ab1     1 NNNNNNNNNN NCNNNNNCTC TTTTTTCTCT CTGCACCNCT CTTCTCTTTG CCTTGGTGGG    61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT   121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG   181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC   241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT   301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT   361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA   421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT   481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG   541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT   601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC   661 CTGTTCGGGC GCCACTGCTA GAGANTTTCC ACACTGACTA AAAGGGTCTG ANGGNTCTCT   721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA NGCAAGCTTT   781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCNAGANAG CTCCNANGCT CANATCTGGT   841 CTAANNAGAN ANANCNANTA CANGCAAAAC GCGCTGCTTA TATAGANCTC CNNNNNNACA   901 CGCNNNNNNN CNNTTTGCGT CANNGGGGGC GGNNTTGNTN NNANNTTTNN NNAANNCCNG 961 NTNNNNTTTN NNGNNNAANN NNNNNNNN //
Primer: env_F (Pr0258) Region: cTTP_CTS,CMV en.2,CMV pro.2 Sequence file: RDB16942_A9Ctm.seq
>15454_16942_A9Ct_env_F_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCGN NNNGNNNNNG NNCTNCAAAT GGCAGTATTC ATCCACAATT    61 TTAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA GACATAATAG   121 CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA AATTTTCGGG   181 TTTATTACAG GGACAGCAGA AATTCACTTT GAATTAATTC GATTAATAGT AATCAATTAC   241 GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG   301 CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC   361 CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC   421 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA   481 TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC   541 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA   601 CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA   661 CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA   721 CTCCGCCCCA TTGACGCAAA TGGGCGGTAN GCGTGTACGG TGGGANGTCT ATATAAGCAG   781 AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCNTANGATC CACCATGGNG AGCAANGGCG   841 ANGNNGNNAA CNTGGCCNTC ATCNANGANT TCATGCGCTT NAAGGTGCAC ATNGANGNNT   901 CCNNNNNCNG NCNCNANTTC GANNTNNNNG NCGANGNNNA NGGNCNNNNN NNCNANGNNN 961 NCCNNNNNNN NNN //
Primer: mCherry-M_F1 Sequence file: RDB16942_A9Ctn.seq
>15454_16942__B2JdF_mCherry-M_F1_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNANNAN NNNCNTGGGC TGGGNNGNCT    61 CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAG CAGAGGCTGA   121 AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC CACCTACAAG GCCAAGAAGC   181 CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC TCCCACAACG   241 AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC ACCGGCGGCA   301 TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC GCTCGAGATG GAGCAGCGCC   361 GCGTCACCGA CTTCTTCGCG CGCCGCCGCC CCGGGCCCCC CCGCATCGCG CCGCCCAAGC   421 TGGCCTGCCG CACCCCCAGC CCCGCCAGGC CCGCACTCCG CGCCCCGGCC TCCGCTACCA   481 GTGGCAGCCG CAAGCGCGCC CGCCCGCCCG CCGCCCCCGG ACGCGACCAG GCCAGGCCAC   541 CGGCCCGCGC GGCCGCGCGG CTGTCGGTGG ACGAGGTTTC CAGCCCCAGT ACCCCCGAGG   601 CCCCAGACAT CCCAGCCTGC CCTTCTCCGG GCCAGAAGAT AAAGAAAACC GGTCGCGCCA   661 AGCGCGGCAG CGGCGCCACC AACTTCAGCC TGCTGAAGCA GGCCGGCGAC GTGGAGGAGA   721 ACCCCGGCCC CCGCGAATTC ATGGTGAGCA AGGGCGAGGA NCTGTTCACC GGGGTGGTGN   781 NNNNNCTGGT CGAGCTGGAC GGCGNNNNNA ACGGCCACAA GNTNNNCGTG TCCGGCGANG   841 GCGANGGCGA TGCCACCTAC NGNNANNCTG ANCCTGAANT TCATCTGCAC NNCNNGGCNN   901 GCTGCCCGTG CCCCTGGNCN ACCNNNNTGA NCACCCNGNN CTGGGGNGNG CAGNGCTTCG   961 CCCGCTACCC CCGANCNCNT NNANNNNNCN CNNNTTNNNC ANNNNCNGCC ATGNCNNNNN 1021 NNTNNNNNNC NNNNCNNNNN NNNNN //
Primer: EGFP-M_R Sequence file: RDB16942_A9Cto.seq
>15454_16942_B2JdF_EGFP-M_R_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNTNN NNNNNNNNCT TGNNGTTGGC CTTGNNGCCG TTCTTCTGCT    61 TGTCGGCGGT GNNATAGACG TTGTCGCTGA TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA   121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC   181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTAC   241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT   301 CGGGGTAGCG GGCGAAGCAC TGCACGCCCC AGCTCAGGGT GGTCACGAGG GTGGGCCAGG   361 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT   421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA   481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG AATTCGCGGG   541 GGCCGGGGTT CTCCTCCACG TCGCCGGCCT GCTTCAGCAG GCTGAAGTTG GTGGCGCCGC   601 TGCCGCGCTT GGCGCGACCG GTTTTCTTTA TCTTCTGGCC CGGAGAAGGG CAGGCTGGGA   661 TGTCTGGGGC CTCGGGGGTA CTGGGGCTGG AAACCTCGTC CACCGACAGC CGCGCGGCCG   721 CGCGGGCCGG TGGCCTGNCC TGGTCGCGTC CGGGGGCGGC GGGCGGGCGG GCGCGCTTGC   781 NGCTGCCACT GGTANCGGAN GCCGGGGCGC GGANTGCGGN CCTGGCNGGG CTGGGGGTGC   841 GGCANGCCAG CTNNGNNGGC GCGATGCNGG GGGGGNCCNG GGNNGNCGGC GCNCNAANAA   901 NTCNGNGANN CNGCGCTGCN NCATCTCGAG CNGNCGCNNN NNNNGANNGN NTNTCCCNTN 961 GTNCANCTCN NNNATGNCNN NNNGGNNNNN NNNNN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA,3'LTR Sequence file: RDB16942_A9Ctp.seq
>15454_16942_A9Ct_BGH_rev2_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGG GGNNGGGCAN NAACAGATGG CTGGCAACTA GAAGGCACAG    61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG   121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC   181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC   241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC   301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA   361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT   421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT   481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG   541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC   601 TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT   661 ACCTGANGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA   721 TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC TANGTCTCGA   781 CNGTATCGAT GCNGGGANGC GGNCCNAAGG GANNTNCGAN TCNTCTGANG NCGAANNNNA   841 ANANNCNGAA GANNNNNCAN ANCCNGNANC NNNCGCNNNA ANGNNNNNNC NNTNNNNTGA 901 NGNNNNNANN NNNNTNNCAN NNNNANNNNN NNNNN //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori,EM7 pro,Zeo,SV40 pA Sequence file: RDB16942_A9Ctq.seq
>15454_16942_A9Ct_SV40pro_F_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNA NNNNTGNNGN NGNTTTTTTG GANGCCTAGG CTTTTGCAAA    61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT   121 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA   181 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT   241 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC   301 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC   361 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT   421 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG   481 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC   541 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT   601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG   661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA   721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCN   781 AACTCATCNA TGTATCTTAT CATGTCTGTA TANCGTCNAN CTCTANCTAG ANCTNGGCGT   841 AATCATGGNC ATANCTGTTT CCTGNGTGAA ATTGNTATCC GCNCACAATN CCNCACAACA   901 TACGAGCCNG AANCNTAAAG TGNNAAGCNN GGGGNNNCNN NNGNNTGAGC TNANNCNNAT 961 TNANNNNNNN TNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.04.04

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