Please review the QC test results indicated by icon below as well as clone information before placing your order.

tFucci(CA)2/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_mVenus-hGem(1/110). CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15452_A7Jvp1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mRFP-M_R (Pr0674) Region: insert 5'(tFucci(CA)2 5',CMV2) Sequence file: RDB15452_A7Jvg.seq
>15452_15452_B2G7F_1_mRFP-M_R_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNCTTGGNN CCGTNNNTGA ACTGAGGGGN NAGGATGTCC    61 CAGGCGAAGG GCANGGGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG   121 TAGGGGCGGC CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC GGAGCCCTCC   181 ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGATGGCCA TGTTATCCTC CTCGCCCTTG   241 CTCACCATGG TGGATCCTAT GCTAGCGGAT CTGACGGTTC ACTAAACCAG CTCTGCTTAT   301 ATAGACCTCC CACCGTACAC GCCTACCGCC CATTTGCGTC AATGGGGCGG AGTTGTTACG   361 ACATTTTGGA AAGTCCCGTT GATTTTGGTG CCAAAACAAA CTCCCATTGA CGTCAATGGG   421 GTGGAGACTT GGAAATCCCC GTGAGTCAAA CCGCTATCCA CGCCCATTGA TGTACTGCCA   481 AAACCGCATC ACCATGGTAA TAGCGATGAC TAATACGTAG ATGTACTGCC AAGTAGGAAA   541 GTCCCATAAG GTCATGTACT GGGCATAATG CCAGGCGGGC CATTTACCGT CATTGACGTC   601 AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT GCCAAGTGGG CAGTTTACCG   661 TAAATACTCC ACCCATTGAC GTCAATGGAA AGTCCCTATT GGCGTTACTA TGGGAACATA   721 CGTCATTATT GACGTCAATG GGCGGGGGTC GTTGGGCGGT CAGCCAGGCG GGCCATTTAC   781 CGTAAGTTAT GTAACGCGGA ACTCCNTATA TGGGCTATGA ACTAATGANC CCGTAATTGA   841 TTACTATTAN TCGAATTAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATN AANCCGAAAA   901 NTTTGANNTT TTGNAANTNG NTTTTGTNAN TTCTTTANTT TGNATGTCTG TTNNTATTAT   961 GTCTACTATT CNTTCCCCTG CACNGNNNCC CCCNATNCCC CCNTTTTCNT TTAAAANTNG  1021 NNGNANGAAN NNTGNNNNTT TGNNNNANNN NNNNNNACTN ANTNNNANNN NNNNNNGNNN 1081 NNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,insert 3'( tFucci(CA)2 3') Sequence file: RDB15452_A7Jvh.seq
>D05018B3_1_A7Jv_PRE_rev_E12_15_ABI24.ab1     1 NNNNNNNNNN NNNGNNNNNG NNCNNCATAG TTAAGAATAC CAGTCAATCT TTCNCAAATT    61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTACA   121 GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA TTTTCTTTAA   181 TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA CTAGATTCTG   241 GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA TGTTTCCTTT   301 TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT GCAGAAGGCT   361 GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT TTGATTTCTT   421 CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT GGATATCCCT   481 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA   541 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGCTTGGA CTGGTAGCTC AGGTAGTGGT   601 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA   661 GCTGCACGCC GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT   721 TCTGCTTGTC GGCGGTGATA TAGACGTTGT GGCTGTTGTA NTTGTACTCC AGCTTGTGCC   781 CCANGATGTT GCCGTCCTCC TTNNANNCNA TGNCCTTCAN CTCNNNGCNN NNCANNANGG   841 NNNCGNCNNN NANCTTNNNN NCNGNNNGGG NNNNGNANTN NNNNNCNNNN NTNNNNNNNN 901 NN //
Primer: mCherry-C_F (Pr0453) Region: insert mid (tFucci(CA)2 mid) Sequence file: RDB15452_A7Jvi.seq
>D05018B3_1_A7Jv_mCherry-C_F_G03_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNCNNN CNGGNGCCTA CAACGTCNAC ATCAAGTTGG ACATCACCTC    61 CCACAACGAG GNCTACACCA TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC   121 CGGCGGCATG GACGAGCTGT ACAAGGGATA TCCATCACAC TGGCGGCCGC TCGAGATGGA   181 GCAGCGCCGC GTCACCGACT TCTTCGCGCG CCGCCGCCCC GGGCCCCCCC GCATCGCGCC   241 GCCCAAGCTG GCCTGCCGCA CCCCCAGCCC CGCCAGGCCC GCACTCCGCG CCCCGGCCTC   301 CGCTACCAGT GGCAGCCGCA AGCGCGCCCG CCCGCCCGCC GCCCCCGGAC GCGACCAGGC   361 CAGGCCACCG GCCCGCGCGG CCGCGCGGCT GTCGGTGGAC GAGGTTTCCA GCCCCAGTAC   421 CCCCGAGGCC CCAGACATCC CAGCCTGCCC TTCTCCGGGC CAGAAGATAA AGAAAACCGG   481 TCGCGCCAAG CGCGGCAGCG GCGCCACCAA CTTCAGCCTG CTGAAGCAGG CCGGCGACGT   541 GGAGGAGAAC CCCGGCCCCC GCGAATTCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG   601 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC   661 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGCTGA TCTGCACCAC   721 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGGGCTACG GCCTGCAGTG   781 CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCNAGTCCG CCNNGCCCGA   841 NNNCTACNNC CANGANCGCN NCATCTTCTT CNANGACGAC NGGCAACNAC NAGANCNGCG   901 CCNANGNNAA NTNCNNNGGG CNANACCCNG NNNNANNCGC ATCNANCNNA ANGGNNTCNN 961 NNNNNNNN //
Primer: HIV-B_R (Pr0673) Region: HIV- psi,5'LTR,CMV1 Sequence file: RDB15452_A7Jvj.seq
>15452_15452_B2G7F_1_HIV-B_R_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNTTTG NNNNNNNNNN NNNNCGCCGC    61 CCCTCGCCTC TTGCCGTGCN CNCNTCANCA AGCCGAGTCC TGCGTCGAGA GAGCTCCTCT   121 GGTTTCCCTT TCGCTTTCAA GTCCCTGTTC GGGCGCCACT GCTAGAGATT TTCCACACTG   181 ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA   241 CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG   301 AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAACGCGCTG   361 CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG GGCGGAGTTG   421 TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC ATTGACGTCA   481 ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC ATTGATGTAC   541 TGCCAAAACC GCATCANCNN GNTAANNAGC GATGACTAAT ACNTAGATGT ACTGCCNAGT   601 AGGAAAGTCC CATNANNGTC ATGTACTGNG CNNNAATGCC AGGNNGNNNN NTTACCGTCN   661 NTGACGTCNA TAGGGGGCGN ANNTGGCATA TGATANNNTT GATGNNNNGN NNANNGGGCA 721 GNTNNNNNNN AANTNCTCNN NCCNNNNNNN NNN //
Primer: HIVgp_R1 (Pr0132) Region: HIV- psi,5'LTR Sequence file: RDB15452_A7Jvk.seq
>D05018B3_1_A7Jv_HIVgp_R1_A12_03_ABI24.ab1     1 NNNNNNNNNN NNCNNNNCNC TTTTTTCTCT CTGCNCCACT CTTCTCTTTG CCTTGGTGGG    61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT   121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG   181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC   241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT   301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT   361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA   421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT   481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG   541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT   601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC   661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA NNANGGTCTG ANGGNNCTCT   721 AGTTACCAGA NTCNCACANC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT   781 ATTGAGGCTT AAGCAGNGGG TNNCCNANTT AGNNNGANAN CTNCNNNGCN CANANCNNGN   841 CTAACNNGAN ANANCNNNNA CNNNNNAAAA NGCGCNNNNN NATATANNNN NNCCNNNNNN 901 NNNN //
Primer: PPT/CTS-E_R (Pr 725) Region: cTTP_CTS Sequence file: RDB15452_A7Jvl.seq
>15452_15452_B2G7F_1_cPPT+CTS-E_R_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTNN NTTTGTATGT CTGTTGCTAT TATGTCTACT    61 ATTCTTTCCC CTGNNCTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAATT GTGGATGAAT   121 ACTGCCATTT GTAGATCCGT TCACTAATCG AATGGATCTG TCTCTGTCTC TCTCTCCACC   181 TTCTTCTTCT ATTCCTTCGG GCCTGTCGGG TCCCCTCGGG GTTGGGAGGT GGGTCTGAAA   241 CGATAATGGT GAATATCCCT GCCTAACTCT ATTCACTATA GAAAGTACAG CAAAAACTAT   301 TCTTAAACCT ACCAAGCCTC CTACTATCAT TATGAATAAT TTTATATACC ACAGCCAATT   361 TGTTATGTTA AACCAATTCC ACAAACTTGC CCATTTATCT AATTCCAATA ATTCTTGTTC   421 ATTCTTTTCT TGCTGGTTTT GCGATTCTTC AATTAAGGAG TGTATTAAGC TTGTGTAATT   481 GTTAATTTCT CTGTCCCACT CCATCCAGGT CGTGTGATTC CAAATCTGTT CCNNAGATTT   541 ATTACTCCAA CTAGCATTCC AAGGCACAGC AGTGGTGCAN ATGANTTTTC CAGANCAACC   601 CCAAATCCCC AGGANCTGTT GATNCTNNAN GTATCTTTCN NNNNCCNGGN NTCNTNNCTG   661 GANCTGCTTG ATGNCCCNNN ANNGTNANTT NNANNNNANG CNGNTGNNNC NNNNNANCCC 721 TNNNNNAATN NNNNNNNNGN NNNNNNNNN //
Primer: CSII-CMV5' (Pr0021) Region: insert mid(tFucci(CA)2 mid) Sequence file: RDB15452_A7Jvm.seq
>D05018B3_1_A7Jv_CSII-CMV5'_C12_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NANGGNNNNC ANGGGCGNNG NNGNNAACAT GGCCATCATC    61 AAGGNGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG   121 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG   181 ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC   241 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC   301 GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC   361 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC   421 TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG   481 CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG   541 GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG   601 CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC   661 ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAN   721 CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGA TGGANCAGCG CCGCGTCACC   781 GACTTCTTCG CGCGCCGCCG CCCCGGGCCC CCCCCGCATC GCGCCGCCCA AGCTGGCCTG   841 CCGCACCCCN NAGCCCNGCC NNGNCCGCAC TCCGCGCCCC NGCCNNCGCN ACCAGNGNNA 901 GCCNCNAGNN NGNCNGCCNG NCCNNNNNNN NCNNNNNNNN ANNNNNNNNN N //
Primer: EGFP-B_R2 (Pr 1232) Region: insert mid (tFucci(CA)2 mid) Sequence file: RDB15452_A7Jvn.seq
>15452_15452_B2G7F_1_EGFP-B_R2_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCNNCNNG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG    61 TAGTGGTCGG CGAGCTGNNN GCCGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTGGCC   121 TTGATGCCGT TCTTCTGCTT GTCGGCGGTG ATATAGACGT TGTGGCTGTT GTAGTTGTAC   181 TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG   241 CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG   301 TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG   361 TCGTGCTGCT TCATGTGGTC GGGGTAGCGG GCGAAGCACT GCAGGCCGTA GCCCAGGGTG   421 GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATCAG CTTCAGGGTC   481 AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT   541 ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG   601 CTCACCATGA ATTCGCGGGG GCCGGGGTTC TCCTCCACGT CGCCGGCCTG CTTCAGCAGG   661 CTGAAGTTGG TGGCGCCGCT GCCGCGCTTG GCGCGACCGG TTTTCTTTAT CTTCTGGCCC   721 GGAGAANGGC AGGCTGGGAT GTCTGGGGCC TCGGGGGNNN NGGGGCTGGA AAACCTCGTC   781 CACCGANNNN NGCGCGGCCG CNCGGGCCGG TGGCCTGNCC TGGTCNCGTC CGGGGGCGGC   841 GGGCGGGCGG GCGCGCTTGC GGCTGCCACT GGTAGCGGAG GNCGGGGCGC GGANTGCGGG   901 CNNGGCGGGG CTGGGGGGTG CGGCANNNNA NCTTNNGGCN GNNCGATGNN NGGGGGGNCC 961 NNGGGNGGCN NNNCNCNNAN ANNTNNNTNA NNNNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: 3'LTR,PRE Sequence file: RDB15452_A7Jvo.seq
>15452_15452_B2G7F_1_bGH_rev3_A02_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG GGGGNNGGNN NNNNACAGAT GGCTGGCAAC TAGAAGGCAC    61 AGTCGAGGCT GNNCNNCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA   121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA   181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT   241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG   301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT   361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT   421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT   481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT   541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC   601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG   661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC   721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC   781 GACGGTATCG ATGCGGGGAG GCGGCCCANA GGGAGATCCG ANTCGTCTGA NGNCGAANGC   841 GAANANNCGG AAGANGNCGC ANANCCNGCA NCNNGNCNCG GGAAGGAANG NCCGCTGGAT   901 TGAGGGNCGA AGGGANNTAN CANANNNNNT NCCGCGCANA ATNCAGGNGG NNACNCANGC   961 GANCAGCCAT NGNAAAGNNN GNCNNCTTNC CCNNNNNNNN CNNNNAATNG NNANTGNCCN 1021 ANAGCCNANC CNNNGNNCAN NNNCNGGNNA NNNNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: EM7 pro,Zeo,SV40 pA Sequence file: RDB15452_A7Jvp.seq
>D05018B3_1_A7Jv_SV40pro_F_B12_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTGN NGNNGCTTTT TTGGANGCCT AGGCTTTTGC    61 AAAAAGCTCC CGGGANCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT   121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG   181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT   241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG   301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA   361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG   421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT   481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG   541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG   601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC   661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA   721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT   781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACNGTC NACCTCTANC TAGANCTTGG   841 CGTAATCATG GTCATANCTG TTNCCTGTGT GAAATTGTTA TNNGCNCNCA ATTNCNCACA   901 ACATACNANC NNGAANCATA AAGNGNNNNN NCNGGGGGNN CCNNANNNNN NNNNCNNNNN 961 NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.04.04

HTML_filter_RDB_240329.pl