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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-T7-mouse GARI-L4

Expression vector of mouse GARI-L4, N-terminal T7-tag.

Catalog number RDB15396
Resource name pEF-T7-mouse GARI-L4
Clone info. Expression vector of mouse Golgi-associated Rab2B interactor-like 4 (GARI-L4) [Fam71a in NCBI], tagged with N-terminal T7, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-T7 (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Fam71a cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
mouse Fam71a
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aizawa, M. and Fukuda, M., J. Biol. Chem. 290 (36): 22250-22261, 2015)
          Additional terms and conditions:
          Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aizawa, M. and Fukuda, M., J. Biol. Chem. 290 (36): 22250-22261, 2015)。
          付加的提供条件：
          pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15396 pEF-T7-mouse GARI-L4 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEF-T7-mouse GARI-L4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15396).

          Reference section:

          Aizawa, M., Fukuda, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15396_A7Ibp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F (Pr0168)
          Region: EF-1 alpha pro,insert(T7 tag,GARI-L4 5')
          Sequence file: RDB15396_A7Iba.seq check
          >D04916C5_A7Ib_2_EF1a-C_F_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTTNGGNN CTTGGTTNNT TCTCAAGCCT CAGACAGTGG
             61 TTCAAAGTTT TTTTCTTCCN TTTCAGGTGT CGTGAGGAAT TCTCTAGAGC GGCCGCGGGA
            121 ATTCGATTCC ACCATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGCG GATCCATGGA
            181 CGTAAAGGAA GAGTCTCTGT TACCATATTA TACAGCCCAG AGTGGTGCTG AAGTCGGCAT
            241 GTTCAACGGC TTGTTCGACA CCGCCACGGG GAAATTACAG CAGCAGTTGC ACAAAGGAGA
            301 GTATGATATA TTCAAGTACG CGCCGATATT CGAGAGCGAC TTCATCCAGA TCACCAGATA
            361 TGGAGAAGTG ATCGACGTGC ACAATCGTGT CCGAATGGTG ACTGTGGGCA TTGCGTGCAC
            421 CAGTCCCATC CTTCCACTCC CTGATGTCAT GCTGCTGGCC CGACCAGCAC CATTCGATGA
            481 GCTTCTGGGA CATGGCCAGT CCACCAACAG CACCAAAGGC AAAAAACGCA AGGCTGGAAA
            541 GACCTTGGAG CTCACTAGGC TCCTTCCTTT GAAGTTTGTA AGGATTTCTG TTCACAGACG
            601 TGATGAACAA CAGCTACGTC TCAAGTTTGC TACTGGCCGT TCTTGCTACC TGCAGTTGTG
            661 CCCCCCTCCC GAAAGCAAGG ATGATCTCTT TTCCTACTGG GAAAAATTGA TTGACCTCCT
            721 GAGACCACCT GTGGACAGTA ACACCAGTAC CTATGCCATC CCAGCANGGG ACATGATGAG
            781 CACGCCTTTT CTGGATGAAG AACTGAAGAT ACCAAAAAAA AAANGCAAAA GAAAATCCGG
            841 CCACCNAAAA AAAAGANATC TATACAANGT CAGCGNCAAA AGCCTCCCCN CGANCACAGA
            901 TGTNNNNGCG ATCAGCNCTT CNGCTTTTTT CNNGNGGGGN NGNAAANCCN NNANNNTCAN
          961 ATNNNNNNNA NNNNNNNNNN
          //
          Primer: hGCSF_R (Pr0031)
          Region: insert(GARI-L4 3')
          Sequence file: RDB15396_A7Ibb.seq check
          >D04916C5_A7Ib_2_hGCSF_R_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNGGGGNNCC TCACTCTAGN NCGGCCGCGA
             61 ATTCACTAGT GATTTTAATG AGCTTCAAAG GTCACTGTCT CCATGACCTC GGATGTCACA
            121 GAATCAATGA TTTCCAGGCC CTGGCCATCC TCTGCCTCCA TGATGGCCTT TACGTCGGTT
            181 GTCTCTAATG TCTTAGTCAT GATGCCCACA TCTTGGCCAC GTGGTGCCAG GCTTTTTCCA
            241 GCCAAGCTGG CTCTGATGTT TCTCAGGAAC AAGCTGATTC TGCCCACCCT CTTGCTCGTA
            301 GAGGCAGAGC TGGCAGTGGA GATGGTCCGG GACAGTTTGT GAGAGCTCCG GGACTCCTTT
            361 GTGATGGGGA CATGGCTGAC CCCTTTGTGC CACCTATTGC GCTTGCTGCC TTTGTCCTTG
            421 TCATCGCCAA CACCTCTGTG ACTGGTTGAT TTTTGAAACT CCTTGTCCCC TTCTGTCTTG
            481 TGACCTTCTC CTGTCCTCTG GTGCTGCTCA CTCCTGCTGC CAACTCTTTC CTTTGCTCTC
            541 TGGTCCCTTC TCGACCTCCT TTCCTTATGG GCTTTGGTAC TGGTCTCATA GGCTTTCCGA
            601 CGTCCAGTCT CTTCATTTGA GTGGCTTTCG CTTGGCAAGG TCAAGGCGAG GACAACTGGG
            661 ACTGGGTCAT TTGCTGTTTC TCCAACAGAC TTTCCTGTAN TCTCAATCCT GGAATTTGTT
            721 ACAGTCATGA TCTCTTTGCG GGANANGCTG GCAGGTGAAC TGGGAATATA CTCCAAGGAC
            781 TTGCTTGCCA TCCCTGCCAA GGNAATCTTC ATGCTCTTTG GCGACATTTT GCCAGTGCTT
            841 GCTATGGTCA AATGGCTTTT GCTTCCTCTT GCTCCCNANG GCANCCTCCT CTACTGNANC
            901 TTTNTTTATC TGNCCCNAGC CTTCNNNNTG NTNGCTGCCN ANCTCAAAGA NTCTACNNNN
          961 NTCACACNCT ANTNNCNNNT GNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl