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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMRX-puro-HA-mouse PAT4

Retroviral expression vector of mouse PAT, N-terminal HA-tag.

Catalog number RDB15392
Resource name pMRX-puro-HA-mouse PAT4
Clone info. Retroviral expression vector of mouse proton assisted amino acid transporter 4 (PAT4) [Slc36a4 in NCBI], tagged with N-terminal HA, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-IRES-puro (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Slc36a4 cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Slc36a4

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Matsui, T. and Fukuda, M., EMBO Rep. 14 (5): 450-457, 2013)
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Matsui, T. and Fukuda, M., EMBO Rep. 14 (5): 450-457, 2013)。
          付加的提供条件:
          pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15392 pMRX-puro-HA-mouse PAT4 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMRX-puro-HA-mouse PAT4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15392).

          Reference section:

          Matsui, T., Fukuda, M., Rab12 regulates mTORC1 activity and autophagy through controlling the degradation of amino-acid transporter PAT4. EMBO Rep. 14 (5): 450-457 (2013). PMID 23478338.

          Further references such as user reports and related articles (go to bottom)


          References and tips

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15392_A7Ibp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMLV-pol_F (Pr0352)
          Region: pol,insert 5'(HA,mPAT4 5')
          Sequence file: RDB15392_A7Iba.seq check
          >15392_15392_B2C2F_MMLV-pol_F_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNGGNATCG CAGCTTGGAN ACNCGCCGCC CACGTGAAGG CTGCCGACCC
             61 CGGGGGTGGA CCATCCTCTA GACTGCCGGA TCTCCACCAT GGGTTACCCA TACGACGTCC
            121 CAGACTACGC TGGATCCATG GAAGCGCCGG CGCCGGCGGA GACGGCGGGA TGCGAGGAGC
            181 TCGATATGGA CGTGATGAGG CCCTTAATAA ACGAGCAGAA TTTCGATGGG TCGTCCGACG
            241 AGGAGCAGGA ACAGACGCTT GTGCCCATAC AGAAGCACTA CCAGCTCGAT GGGCAGCACG
            301 GGATTTCGTT CCTGCAGACC TTGGTGCATC TTCTCAAGGG GAACATCGGG ACTGGCCTTT
            361 TAGGGCTCCC CTTGGCTATA AAGAACGCGG GCATCGTGCT TGGACCAATC AGCCTTGTGT
            421 TTATAGGAAT TATTTCCGTC CACTGTATGC ACATATTGGT ACGTTGCAGT CACTTTCTAT
            481 GTCAGAGGTT TAAGAAGTCA ACATTGGGGT ACAGTGACAC CGTGAGTTTT GCCATGGAGG
            541 CTAGTCCGTG GAGTTGCCTT CNGCGACAGG CAGCATGGGG GCGGANCGTG GTCNACTTCN
            601 TTCTGGTGAT AACNCNGCTG GGNNTCNGCA GCGTCTACNT TGNCNTCNNA NCTGANANTG
          661 NGNAANNNNN NNNTNNNNNN NNNN
          //
          Primer: IRES-F (Pr0036)
          Region: IRES ,PuroR 5'
          Sequence file: RDB15392_A7Ibb.seq check
          >D04916C1_A7Ib_1_IRES-F_F08_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNTNNGT CGAGGTTNNN AAACGTCTAG GCCCCCCGAA CCNCGGGGAC
             61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
            121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC TCGCCGCCGC
            181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA TCGAGCGGGT
            241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
            301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
            361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
            421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
            481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
            541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
            601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
            661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC
            721 GCAGCGCCCG ANCGAAAGGA GCGCACGANC CCATGGCTCG ACGATAAAAT AAAAGATTTT
            781 ATTTAGTCTC CAGAAAAAGG NNGGAATGAA AGANCCCACC TGTANGTTTG GNAAGCTAGC
            841 TTAAGTNACG CCNTTTTGCN AGGNATGGAA AAATACNTAA CTGANAATAG ANAANNNCNN
            901 ATCAAGGTCN NNAACAGATG GNNCAGCTGN ANATGGGNCA AACNNNANAT CTGNGGTAAG
            961 CAGTTNCNGN CNNNGNTCNN NNNNANANNA NANGGNNNAG CTNNNNTGGN NNNANNNNNN
          1021 NNNN
          //
          Primer: IRES-R (Pr0037)
          Region: insert 3'(mPAT4 3')
          Sequence file: RDB15392_A7Ibc.seq check
          >15392_15392_B2C2F_IRES-R_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNANCG TTAGGGGGGG GGGCGGAATT TACGTAGCGG CCGCGAATTC
             61 ACTAGTGATT TTGATCATTA CAAACCACTT GTTATGCATG TAGAGTTCAC ATTCAGAAAG
            121 GGACTCTGGG AGGTGCCAGC AACAACTGCC GTAGTCGGAT AAATAATTTC TTCAACAGTG
            181 ACATAGGTGC CCAACAAGAA GCCCACGACT CCAGTGAACG CTATGGAAAT GTTTTTCAGG
            241 ATCATCCATA TGTTGTAGTG GTCCTTAGAA AATGTAAGGA TTTCCACAAG TGGGGGCAGG
            301 ATCAGGGCTA GTGTACTGCT GCTCACAGCC CCCACGAAGG AGATCACAAT GTCTAGGCGA
            361 GGAATGAGAA TCGCCCCAGC ACAGGTGATA CTAACCAAGA GGGACCGTAT CCCAAATTCA
            421 CAAATGCGCT TCCATTTGGC ATGAAGTCTA GCAGTGACTC CAGGGATAAT GATCTCTGCT
            481 GGGACATAGA ACTGAATTGA ATAGGTCACA AAAATGCCAA AGGAATACAG AATTTTCACT
            541 GACTGATACA ACCACATGTC CTGGGGAAGA TTCAGTGTAA TGCTGCCTTT GATCTCGTCN
            601 CGGAAACACA TGTAGCCTAA AGTGGCTAGG CTGANGTACA GCACGGNGAC GATGGNNNTG
            661 CCNATGTTCN NNGNCTGNNG NNNNGTTTGG NNTCTCTNNT TTGGTTTTCC NNNNNNANCN
          721 NNNNNNNNN
          //
          Primer: MMLV psi_F (Pr0863)
          Region: MMVV psi,gag,pol
          Sequence file: RDB15392_A7Ibd.seq check
          >15392_15392_B2C2F_MMLVpsi_F_C01_03_ABI08.ab1
              1 NNNNNNNNNC NNNNNNNNNN NTTTNCTTTC GGTTTGGGAC CGAAGCCGCG CCGCGCGTCT
             61 TGTCTGCTGC AGCATCGTTC TGTGTTGTCT CTGTCTGACT GTGTTTCTGT ATTTGTCTGA
            121 AAATATGGGC CCGGGCCAGA CTGTTACCAC TCCCTTAAGT TTGACCTTAG GTCACTGGAA
            181 AGATGTCGAG CGGATCGCTC ACAACCAGTC GGTAGATGTC AAGAAGAGAC GTTGGGTTAC
            241 CTTCTGCTCT GCAGAATGGC CAACCTTTAA CGTCGGATGG CCGCGAGACG GCACCTTTAA
            301 CCGAGACCTC ATCACCCAGG TTAAGATCAA GGTCTTTTCA CCTGGCCCGC ATGGACACCC
            361 AGACCAGGTC CCCTACATCG TGACCTGGGA AGCCTTGGCT TTTGACCCCC CTCCCTGGGT
            421 CAAGCCCTTT GTACACCCTA AGCCTCCGCC TCCTCTTCCT CCATCCGCCC CGTCTCTCCC
            481 CCTTGAACCT CCTCGTTCGA CCCCGCCTCG ATCCTCCCTT TATCCAGCCC TCACTCCTTC
            541 TCTAGGCGCC CCCATATGGC CNTATGAGAT CTTATATGGG GCACCCCCGC CCCTTGNAAA
            601 CTTCCCTGAN CCTGACNTGA CAAGAGTTAC TAACAGCCCC TCTCTNCAAG CTNNCTTACA
            661 GGCTCTCTAC TTANTCCNGC ACGAAGTCNG GNNNNNTCNG NNNGCNNCCT ANNNNNNANN
          721 NCNNNNNNNA NNNNNNNNNC NNNNNNNNNN
          //
          Primer: PuroR_F (Pr0554)
          Region: PuroR 3',3' LTR
          Sequence file: RDB15392_A7Ibe.seq check
          >15392_15392_B2C2F_PuroR_F_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNCNNNGTGC CCGAAGGANC GCGCACCTGG TGCATGACCC GNNNGCCCGG
             61 TGCCTGACGC CCGCCCCACG ACCCGCAGCG CCCGACCGAA AGGAGCGCAC GACCCCATGG
            121 CTCGACGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA AAGGGGGGAA TGAAAGACCC
            181 CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT GGAAAAATAC
            241 ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTCAGGAACA GATGGAACAG CTGAATATGG
            301 GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG
            361 AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG
            421 GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG CAGTTTCTAG AGAACCATCA
            481 GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA
            541 TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC
            601 ACAACCCCTC ACTCGGGGCG CCNGTCCTCC GATTGACTGA GTCGCCCGGG TANCCGNGTA
            661 TCCNATAAAC CCTCNNGCAN TTGCNTCCNA NTNGNGGNCT CGCTNNNCNN NNNNNNGNNC
          721 NNNNNNANNG ANNGNNNNNC NNNNNNNNGG GGNNNNNNNN NN
          //
          Primer: Amp_R (Pr0008)
          Region: AmpR pro,CMV en,deltta U3 5'LTR
          Sequence file: RDB15392_A7Ibf.seq check
          >D04916C1_A7Ib_1_Amp_R_G08_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNTGANC ATTNATCNGG GTTATTGTCT CNTGAGCGGA TACATATTTG
             61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
            121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
            181 GGCCCTTTCG TCTTCACTCG AGGAGCTTGG CCCATTGCAT ACGTTGTATC CATATCATAA
            241 TATGTACATT TATATTGGCT CATGTCCAAC ATTACCGCCA TGTTGACATT GATTATTGAC
            301 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG
            361 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
            421 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
            481 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
            541 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
            601 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
            661 CATGCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GANCTGAAAT GANCCTGTGC
            721 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG
            781 AGCTCAATAA AANAGCCCAC AACCCCTCAC TCGGCGCGCC NGTCCTCCGA TTGANTGANT
            841 CGCCCGGGTA CCCGNGTATN CNATAAACCC TCTTGNAGTT GCNTCCGACT TGTGNTCTCG
            901 CTGNTCCTNG GGANGGTCTC CTCTGANNGN NTGANNANCN NNCAGCGGGG GNCTTNNNTT
            961 TGGGGNNTCG NCCNGNNNNN GNNNANCNNN NNCCAGGGNN NANNNANCNN NNNNNNGNNN
          1021 NTNNNNNNGN NNNNNNNNTN NNNNGNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Matsui, T., Rab12 regulates mTORC1 activity and autophagy through controlling the degradation of amino-acid transporter PAT4. EMBO Rep. 14 (5): 450-457 (2013). PMID 23478338.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl