Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-T7-mouse Atg16L1g

Expression vector of mouse Atg16L1, N-terminal T7-tag.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

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Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)。 付加的提供条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15376_A7Ibp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(T7 tag,Atg16L1g 5') Sequence file: RDB15376_A7Iba.seq
>D04916A3_A7Ib_2_EF1a-C_F_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNTTTTGAN TTTGGNNCTT GGTTCNTTCT CAAGCCTCAG ACAGTGGTTC    61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT   121 CGATTCCACC ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGCGGAT CCATGTCGTC   181 GGGCCTGCGC GCCGCAGACT TTCCCCGCTG GAAGCGTCAC ATCGCGGAGG AACTGAGGCG   241 CCGGGACCGA CTGCAGAGGC AGGCGTTCGA GGAGATCATT CTGCAGTATA CCAAGTTGCT   301 GGAAAAGTCA GATCTTCATT CAGTATTGAC CCAGAAACTA CAAGCAGAAA AGCATGACAT   361 GCCAAATAGG CATGAAATAA GTCCTGGACA TGATGGTGCG TGGAATGATA GTCAACTACA   421 AGAAATGGCC CAGTTGAGGA TCAAACACCA GGAAGAGCTG ACCGAACTGC ACAAGAAGCG   481 TGGGGAGTTA GCTCAGTTGG TGATTGACCT GAACAACCAA ATGCAGCAGA AGGACAAGGA   541 GATACAGATG AATGAAGCAA AGATTTCGGA GTATTTACAG ACCATCTCTG ACCTGGAGAC   601 AAACTGCCTG GACCTGCGCA CCAAACTGCA GGACCTCGAG GTAGCCAACC AGACCCTGAA   661 NGANGAGTAT GACGCCCTGC AGATTACTTT TACTGCCCTA GANNAGAAAC TGANGAAAAC   721 TACTGANGAG AACCAGGAAC TGGTCACCAG ATGGATGGCT GANAANGCCC AAGAAGCCNA   781 TCGCCTCAAT GCAGANAATG ANAANGANTC CNGGNNGCGT CAAGCACGGC TGCAGAANGA   841 NCTTGNANAA NCAGCAAANG AACCTCTACC NGNTGAACAN GNNGATGNNN TTNNNGNCNT 901 NNNGGNNGAN NNCNCNNANN NCNCAGNANN ANNNCNNNNN CNNNNNNNNN N //
Primer: hGCSF_R (Pr0031) Region: hG-CSF pA,insert 3'(Atg16L1g 3') Sequence file: RDB15376_A7Ibb.seq
>D04916A3_A7Ib_2_hGCSF_R_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GGGTCCNNGG NGGGGNNCCT CACTCTAGAG CGGCCGCTAT    61 GGTCGACCTG CAGGCGGCCG CGAATTCACT AGTGATTTCA AGGCTGTGCC CACAGCACAG   121 CTCTGCTTCC TTTGTCCACA CTGACAACAT GTAAGCCCGA GGGGGCCCAC GCCACCGCAT   181 TGATAGAAGA GCTGTGCTGT TTTGAAAGAA CCTTCTCCAC TTTCCCTGTC AGCACACTCC   241 AGACATAAAG AGAACCCTCG GCTGAGCCTG CTGCCACGTA ACTGCCATCA GGGCTGAAGA   301 CAACCCGGGT CCAGTCAGAG CCGCATTTGA ATCCAGGCGC ACTGAATGTC TGTTTGACTG   361 CATTTGTCCG GAGGTCGATG ACTTTTAGCA GGTCATCACG GGAGCAGCTC AGGAGCTCAG   421 TTCTCTCAGG GTTTAGGTCC AGAGCAGTGA TCTTCCCTAA CAGTTCCATC TCTCGGACCA   481 CACTCTCTGA CCGGATATCC CAGAAACGAA TTTTCTTGTC AAAATGTCCA CTCATTACAC   541 ATTGTTCAGT GCAAACAATG TCATTGCAGC TGGATCCTGC AAACACTGTT TTTATGCAGA   601 CTTTGCTGCG GAGATCCCAG AGTTTGAGGG TCCGGTCGTG ACTTCCTGAG ACAATCCGTG   661 CATTGTCCAG CAGGAACTTG GCAGANAGGA CTTTCCCGCT GTGGCCTGTG AGTGTGTGCC   721 GTAATCGATA ATCATCNACA GTCCAGATTC GGCTTGCNAA ATCATTTGAA GCTGCTAATA   781 NGTAANCTCC AGCACTATCA AATTCAATGC TTGTAATTCC AGCATTACTG CCNGANAGGG   841 NNCCCTTGAA TTCACATTTA TCTCCNANNG CTNCCCNAAG TTNCACCNNG CGGNNCNNGC 901 NNCCNNNGNN CNNGCAACCN GGANCCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.04.01

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